Reviewed, UniProtKB/Swiss-Prot P63935 (MMSB_MYCTU)

Names and origin

Protein names

Recommended name:
    Probable 3-hydroxyisobutyrate dehydrogenase
      Short name=HIBADH
    EC=1.1.1.31

Gene names

Name:	mmsB
Ordered Locus Names:	Rv0751c, MT0775
ORF Names:	MTV041.25c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH.
Pathway	Amino-acid degradation; L-valine degradation.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Probable 3-hydroxyisobutyrate dehydrogenase		PRO_0000173056
Regions
	Nucleotide binding	3 – 31	29	NAD Potential
Sites
	Active site	168	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63935-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 68DEF129A8BA3E68	FASTA	294	29,679
	10 20 30 40 50 60 MTTIAFLGLG NMGAPMSANL VGAGHVVRGF DPAPTAASGA AAHGVAVFRS APEAVAEADV 70 80 90 100 110 120 VITMLPTGEV VRRCYTDVLA AARPATLFID SSTISVTDAR EVHALAESHG MLQLDAPVSG 130 140 150 160 170 180 GVKGAAAATL AFMVGGDEST LRRARPVLEP MAGKIIHCGA AGAGQAAKVC NNMVLAVQQI 190 200 210 220 230 240 AIAEAFVLAE KLGLSAQSLF DVITGATGNC WAVHTNCPVP GPVPTSPANN DFKPGFSTAL 250 260 270 280 290 MNKDLGLAMD AVAATGATAP LGSHAADIYA KFAADHADLD FSAVIHTLRA RADA

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842574 Genomic DNA. Translation: CAA17518.1. AE000516 Genomic DNA. Translation: AAK45016.1.
PIR	B70825.
RefSeq	NP_215265.1. NP_335202.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	888658. 926076.
GenomeReviews	Gene locus MT0775 in contig AE000516_GR. Gene locus Rv0751c in contig AL123456_GR.
KEGG	mtc:MT0775. mtu:Rv0751c.
TIGR	MT0775.
Organism-specific databases
TubercuList	Rv0751c.
Phylogenomic databases
HOGENOM	P63935.
Family and domain databases
InterPro	IPR002204. 3-OH-isobutyrate_DHase-rel_CS. IPR015815. 3hydroxyacid_DHase/Rdtase. IPR006115. 6PGDH_NAD-bd. IPR013328. DHase_multihelical. IPR011548. IsoBut3OH_DH. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01692. HIBADH. 1 hit.
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMSB_MYCTU

Accession

Primary (citable) accession number: P63935
Secondary accession number(s): O53814

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)