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P63883 (AMIC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylmuramoyl-L-alanine amidase AmiC

EC=3.5.1.28
Gene names
Name:amiC
Synonyms:ygdN
Ordered Locus Names:b2817, JW5449
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors. Ref.3 Ref.6

Catalytic activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Subcellular location

Periplasm. Note: Present throughout the periplasm in non-dividing cells, but localizes almost exclusively to a ring at the site of constriction in dividing cells. Ref.5

Domain

N-terminal domain is required and sufficient for targeting to the septal ring. Ref.5

Post-translational modification

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

Disruption phenotype

Mutants are growing in chains of 3 to 6 cells. Ref.3

Sequence similarities

Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Sequence caution

The sequence AAB40464.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpeptidoglycan catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentperiplasmic space

Inferred from direct assay Ref.5. Source: EcoliWiki

   Molecular_functionN-acetylmuramoyl-L-alanine amidase activity

Inferred from direct assay Ref.3. Source: EcoliWiki

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 417386N-acetylmuramoyl-L-alanine amidase AmiC
PRO_0000006465

Secondary structure

........................................................... 417
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P63883 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 76F8F733DCCC36D7

FASTA41745,634
        10         20         30         40         50         60 
MSGSNTAISR RRLLQGAGAM WLLSVSQVSL AAVSQVVAVR VWPASSYTRV TVESNRQLKY 

        70         80         90        100        110        120 
KQFALSNPER VVVDIEDVNL NSVLKGMAAQ IRADDPFIKS ARVGQFDPQT VRMVFELKQN 

       130        140        150        160        170        180 
VKPQLFALAP VAGFKERLVM DLYPANAQDM QDPLLALLED YNKGDLEKQV PPAQSGPQPG 

       190        200        210        220        230        240 
KAGRDRPIVI MLDPGHGGED SGAVGKYKTR EKDVVLQIAR RLRSLIEKEG NMKVYMTRNE 

       250        260        270        280        290        300 
DIFIPLQVRV AKAQKQRADL FVSIHADAFT SRQPSGSSVF ALSTKGATST AAKYLAQTQN 

       310        320        330        340        350        360 
ASDLIGGVSK SGDRYVDHTM FDMVQSLTIA DSLKFGKAVL NKLGKINKLH KNQVEQAGFA 

       370        380        390        400        410 
VLKAPDIPSI LVETAFISNV EEERKLKTAT FQQEVAESIL AGIKAYFADG ATLARRG 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli."
Heidrich C., Templin M.F., Ursinus A., Merdanovic M., Berger J., Schwarz H., de Pedro M.A., Holtje J.V.
Mol. Microbiol. 41:167-178(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN AMIDASE, DISRUPTION PHENOTYPE.
Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.
[4]"Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: EXPORT VIA THE TAT-SYSTEM AND THE SEC-SYSTEM.
[5]"The Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathway."
Bernhardt T.G., de Boer P.A.
Mol. Microbiol. 48:1171-1182(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, EXPORT VIA THE TAT-SYSTEM, DOMAIN.
Strain: K12.
[6]"Growth of Escherichia coli: significance of peptidoglycan degradation during elongation and septation."
Uehara T., Park J.T.
J. Bacteriol. 190:3914-3922(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U29581 Genomic DNA. Translation: AAB40464.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75856.2.
AP009048 Genomic DNA. Translation: BAE76886.1.
RefSeqNP_417294.4. NC_000913.3.
YP_491022.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BINX-ray2.49A35-417[»]
ProteinModelPortalP63883.
SMRP63883. Positions 35-408.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP63883. 2 interactions.
STRING511145.b2817.

Proteomic databases

PRIDEP63883.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75856; AAC75856; b2817.
BAE76886; BAE76886; BAE76886.
GeneID12932175.
947293.
KEGGecj:Y75_p2751.
eco:b2817.
PATRIC32121052. VBIEscCol129921_2915.

Organism-specific databases

EchoBASEEB2895.
EcoGeneEG13086. amiC.

Phylogenomic databases

eggNOGCOG0860.
HOGENOMHOG000263827.
KOK01448.
OMARAYMTRN.
OrthoDBEOG6CP3X3.
PhylomeDBP63883.
ProtClustDBCLSK880499.

Enzyme and pathway databases

BioCycEcoCyc:G7458-MONOMER.
ECOL316407:JW5449-MONOMER.
MetaCyc:G7458-MONOMER.

Gene expression databases

GenevestigatorP63883.

Family and domain databases

Gene3D3.40.630.40. 2 hits.
InterProIPR021731. AMIN_dom.
IPR002508. CW_Hdrlase/autolysin_cat.
IPR006311. TAT_signal.
[Graphical view]
PfamPF01520. Amidase_3. 1 hit.
PF11741. AMIN. 1 hit.
[Graphical view]
SMARTSM00646. Ami_3. 1 hit.
[Graphical view]
PROSITEPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP63883.

Entry information

Entry nameAMIC_ECOLI
AccessionPrimary (citable) accession number: P63883
Secondary accession number(s): Q2MA20, Q46929
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene