P63872 (CYSK_STAAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Name:	cysK
Ordered Locus Names:	MW0468

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Cysteine synthase		PRO_0000167100
Regions
	Region	180 – 184	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	76	1	Pyridoxal phosphate By similarity
	Binding site	268	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63872 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B881E3E85FF0DBC3	FASTA	310	32,976
	10 20 30 40 50 60 MAQKPVDNIT QIIGGTPVVK LRNVVDDNAA DVYVKLEYQN PGGSVKDRIA LAMIEKAERE 70 80 90 100 110 120 GKIKPGDTIV EPTSGNTGIG LAFVCAAKGY KAVFTMPETM SQERRNLLKA YGAELVLTPG 130 140 150 160 170 180 SEAMKGAIKK AKELKEEHGY FEPQQFENPA NPEVHELTTG PELLQQFEGK TIDAFLAGVG 190 200 210 220 230 240 TGGTLSGVGK VLKKEYPNIE IVAIEPEASP VLSGGEPGPH KLQGLGAGFI PGTLNTEIYD 250 260 270 280 290 300 SIIKVGNDTA MEMSRRVAKE EGILAGISSG AAIYAAIQKA KELGKGKTVV TVLPSNGERY 310 LSTPLYSFDD

References

[1]	"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed: 12044378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MW2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000033 Genomic DNA. Translation: BAB94333.1.
RefSeq	NP_645285.1. NC_003923.1.
3D structure databases
ProteinModelPortal	P63872.
SMR	P63872. Positions 4-310.
ModBase	Search...
Protein-protein interaction databases
STRING	P63872.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000026570; EBSTAP00000025609; EBSTAG00000026569.
GeneID	1002572.
GenomeReviews	Gene locus MW0468 in contig BA000033_GR.
KEGG	sam:MW0468.
PATRIC	19567436. VBIStaAur44266_0496.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0031.
GeneTree	EBGT00050000024422.
HOGENOM	HBG748215.
OMA	SAGANIH.
PhylomeDB	P63872.
ProtClustDB	CLSK884704.
Enzyme and pathway databases
BioCyc	SAUR196620:MW0468-MONOMER.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
KO	K01738.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01139. CysK. 1 hit. TIGR01136. CysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_STAAW

Accession

Primary (citable) accession number: P63872
Secondary accession number(s): Q99W90

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program