Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Oxygen-dependent coproporphyrinogen-III oxidase

Gene

hemF

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation

Catalytic activityi

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116SubstrateUniRule annotation1
Metal bindingi120Divalent cationUniRule annotation1
Active sitei130Proton donorUniRule annotation1
Metal bindingi130Divalent cationUniRule annotation1
Metal bindingi168Divalent cationUniRule annotation1
Metal bindingi199Divalent cationUniRule annotation1
Sitei199Important for dimerizationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00322.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)
Short name:
CPOUniRule annotation
Short name:
Coprogen oxidaseUniRule annotation
Short name:
CoproporphyrinogenaseUniRule annotation
Gene namesi
Name:hemFUniRule annotation
Ordered Locus Names:BR1550, BS1330_I1544
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098881 – 303Oxygen-dependent coproporphyrinogen-III oxidaseAdd BLAST303

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP63851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 134Substrate bindingUniRule annotation3
Regioni268 – 303Important for dimerizationUniRule annotationAdd BLAST36
Regioni286 – 288Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000262768.
KOiK00228.
OMAiIDLTPHY.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas. 1 hit.
InterProiIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63851-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKREDIPAII PADIEEKKKA AQSWFEELRD RICASYEQLE DELQGPLSDR
60 70 80 90 100
EPGRFVRTPW QKDDGNGGGV MSIMHGRVFE KVGVHVSTVH GEFSPEFRKQ
110 120 130 140 150
IPGAEEDPRY WASGISLIAH PQNPNVPAVH MNTRMIVTTR QWFAGGADLT
160 170 180 190 200
PVLDRRRTQE DPDTLAFHKA FRFICEKHKD IVDYQRLKEW CDEYFFLPHR
210 220 230 240 250
DEPRGIGGIF YDWLHSPEEK GGWDSDFAFT RDVGRGFSVV YPHLVRQNFN
260 270 280 290 300
KDWTEADRDE QLIRRGRYVE FNLLYDRGTI FGLKTGGNMN AILSSMPPVV

KWP
Length:303
Mass (Da):35,111
Last modified:October 11, 2004 - v1
Checksum:i65C4C1B754D13CE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30457.1.
CP002997 Genomic DNA. Translation: AEM18873.1.
RefSeqiWP_002964654.1. NZ_KN046804.1.

Genome annotation databases

EnsemblBacteriaiAAN30457; AAN30457; BR1550.
AEM18873; AEM18873; BS1330_I1544.
GeneIDi3788746.
KEGGibms:BR1550.
bsi:BS1330_I1544.
PATRICi17791465. VBIBruSui107850_1577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30457.1.
CP002997 Genomic DNA. Translation: AEM18873.1.
RefSeqiWP_002964654.1. NZ_KN046804.1.

3D structure databases

ProteinModelPortaliP63851.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN30457; AAN30457; BR1550.
AEM18873; AEM18873; BS1330_I1544.
GeneIDi3788746.
KEGGibms:BR1550.
bsi:BS1330_I1544.
PATRICi17791465. VBIBruSui107850_1577.

Phylogenomic databases

HOGENOMiHOG000262768.
KOiK00228.
OMAiIDLTPHY.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00322.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas. 1 hit.
InterProiIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM6_BRUSU
AccessioniPrimary (citable) accession number: P63851
Secondary accession number(s): G0KC31, Q8YIH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.