P63803 (KCY_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytidylate kinase
Short name=CK
EC=2.7.4.25

Alternative name(s):
Cytidine monophosphate kinase
Short name=CMP kinase

Gene names

Name:	cmk
Ordered Locus Names:	Rv1712, MT1752
ORF Names:	MTCI125.34

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + (d)CMP = ADP + (d)CDP. Ref.3
Subunit structure	Monomer. Ref.3
Subcellular location	Cytoplasm By similarity HAMAP MF_00238.
Sequence similarities	Belongs to the cytidylate kinase family. Type 1 subfamily.
Biophysicochemical properties	Kinetic parameters: KM=120 µM for CMP Ref.3 KM=165 µM for dCMP KM=13.9 mM for UMP Vmax=131 µmol/min/mg enzyme with CMP as substrate Vmax=75 µmol/min/mg enzyme with dCMP as substrate Vmax=32 µmol/min/mg enzyme with UMP as substrate
Mass spectrometry	Molecular mass is 23930.56 Da from positions 2 - 230. Determined by ESI. Ref.3

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cytidylate kinase activity Inferred from direct assay Ref.3. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 230	229	Cytidylate kinase HAMAP MF_00238		PRO_0000131943
Regions
	Nucleotide binding	13 – 21	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63803 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: D1E6BF0354D13AA3	FASTA	230	24,177
	10 20 30 40 50 60 MSRLSAAVVA IDGPAGTGKS SVSRRLAREL GARFLDTGAM YRIVTLAVLR AGADPSDIAA 70 80 90 100 110 120 VETIASTVQM SLGYDPDGDS CYLAGEDVSV EIRGDAVTRA VSAVSSVPAV RTRLVELQRT 130 140 150 160 170 180 MAEGPGSIVV EGRDIGTVVF PDAPVKIFLT ASAETRARRR NAQNVAAGLA DDYDGVLADV 190 200 210 220 230 RRRDHLDSTR AVSPLQAAGD AVIVDTSDMT EAEVVAHLLE LVTRRSEAVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"The Rv1712 Locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially phosphorylates dCMP." Thum C., Schneider C.Z., Palma M.S., Santos D.S., Basso L.A. J. Bacteriol. 191:2884-2887(2009) [PubMed: 19181797] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17, CATALYTIC ACTIVITY, SUBUNIT, MASS SPECTROMETRY, BIOPHYSICOCHEMICAL PROPERTIES, REACTION MECHANISM.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842577 Genomic DNA. Translation: CAB10969.1. AE000516 Genomic DNA. Translation: AAK46023.1.
PIR	G70504.
RefSeq	NP_216228.1. NC_000962.2. NP_336209.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P63803.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003003; EBMYCP00000003003; EBMYCG00000003001. EBMYCT00000069081; EBMYCP00000067140; EBMYCG00000069076.
GeneID	885157. 923952.
GenomeReviews	Gene locus MT1752 in contig AE000516_GR. Gene locus Rv1712 in contig AL123456_GR.
KEGG	mtc:MT1752. mtu:Rv1712.
PATRIC	18125606. VBIMycTub22151_1917.
TIGR	MT1752.
Organism-specific databases
TubercuList	Rv1712.
Phylogenomic databases
GeneTree	EBGT00050000015811.
HOGENOM	HBG354377.
OMA	DIGTVIC.
PhylomeDB	P63803.
ProtClustDB	PRK00023.
Family and domain databases
HAMAP	MF_00238. Cytidyl_kinase_type1. [Tree]
InterPro	IPR003136. Cytidylate_kin. IPR011994. Cytidylate_kinase_dom. [Graphical view]
KO	K00945.
PANTHER	PTHR21299:SF2. Cytidylate_kin. 1 hit.
Pfam	PF02224. Cytidylate_kin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00017. Cmk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KCY_MYCTU

Accession

Primary (citable) accession number: P63803
Secondary accession number(s): O33211

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program