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Protein

ATP-dependent Clp protease proteolytic subunit

Gene

clpP

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.UniRule annotation

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981NucleophileUniRule annotation
Active sitei123 – 1231UniRule annotation

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • serine-type endopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-751-MONOMER.

Protein family/group databases

MEROPSiS14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunitUniRule annotation (EC:3.4.21.92UniRule annotation)
Alternative name(s):
Endopeptidase ClpUniRule annotation
Gene namesi
Name:clpPUniRule annotation
Ordered Locus Names:MW0730
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195ATP-dependent Clp protease proteolytic subunitPRO_0000179654Add
BLAST

Interactioni

Subunit structurei

Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-5260070,EBI-5260070

GO - Molecular functioni

  • identical protein binding Source: IntAct

Structurei

Secondary structure

1
195
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105Combined sources
Beta strandi15 – 195Combined sources
Helixi20 – 256Combined sources
Turni26 – 283Combined sources
Beta strandi29 – 324Combined sources
Helixi38 – 5417Combined sources
Beta strandi56 – 583Combined sources
Beta strandi60 – 667Combined sources
Helixi71 – 8313Combined sources
Beta strandi84 – 863Combined sources
Beta strandi88 – 9710Combined sources
Helixi99 – 1046Combined sources
Beta strandi112 – 1143Combined sources
Beta strandi119 – 1224Combined sources
Beta strandi126 – 1327Combined sources
Helixi133 – 15826Combined sources
Helixi162 – 1687Combined sources
Beta strandi173 – 1764Combined sources
Helixi177 – 1826Combined sources
Beta strandi187 – 1893Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ST9X-ray2.43A/B/C/D/E/F/G1-195[»]
3STAX-ray2.28A/B/C/E/F/G/I/K/L/M/N/S/T/V1-195[»]
4EMMX-ray2.40A/B/C/D/E/F/G/H/I/J/K/L/M/V1-195[»]
4EMPX-ray2.70A/B/C/E/F/G/I/K/L/M/N/S/T/V1-195[»]
ProteinModelPortaliP63786.
SMRiP63786. Positions 4-192.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63786.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000285833.
OMAiARMNELM.
OrthoDBiEOG6Z3KQ0.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR00493. clpP. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63786-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLIPTVIET TNRGERAYDI YSRLLKDRII MLGSQIDDNV ANSIVSQLLF
60 70 80 90 100
LQAQDSEKDI YLYINSPGGS VTAGFAIYDT IQHIKPDVQT ICIGMAASMG
110 120 130 140 150
SFLLAAGAKG KRFALPNAEV MIHQPLGGAQ GQATEIEIAA NHILKTREKL
160 170 180 190
NRILSERTGQ SIEKIQKDTD RDNFLTAEEA KEYGLIDEVM VPETK
Length:195
Mass (Da):21,514
Last modified:October 11, 2004 - v1
Checksum:i811110E32846625E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94595.1.
RefSeqiWP_001049165.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB94595; BAB94595; BAB94595.
PATRICi19568014. VBIStaAur44266_0768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94595.1.
RefSeqiWP_001049165.1. NC_003923.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ST9X-ray2.43A/B/C/D/E/F/G1-195[»]
3STAX-ray2.28A/B/C/E/F/G/I/K/L/M/N/S/T/V1-195[»]
4EMMX-ray2.40A/B/C/D/E/F/G/H/I/J/K/L/M/V1-195[»]
4EMPX-ray2.70A/B/C/E/F/G/I/K/L/M/N/S/T/V1-195[»]
ProteinModelPortaliP63786.
SMRiP63786. Positions 4-192.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS14.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB94595; BAB94595; BAB94595.
PATRICi19568014. VBIStaAur44266_0768.

Phylogenomic databases

HOGENOMiHOG000285833.
OMAiARMNELM.
OrthoDBiEOG6Z3KQ0.

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-751-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP63786.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR00493. clpP. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MW2.

Entry informationi

Entry nameiCLPP_STAAW
AccessioniPrimary (citable) accession number: P63786
Secondary accession number(s): Q99VK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.