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P63697 (BFR_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bacterioferritin

Short name=BFR
EC=1.16.3.1
Gene names
Name:bfr
Synonyms:bfrA
Ordered Locus Names:Rv1876, MT1925
ORF Names:MTCY180.42c
OrganismMycobacterium tuberculosis [Reference proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex By similarity.

Catalytic activity

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per dimer. Ref.3

Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center.

Subunit structure

Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited. Ref.4

Sequence similarities

Belongs to the bacterioferritin family.

Contains 1 ferritin-like diiron domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 159159Bacterioferritin
PRO_0000192600

Regions

Domain1 – 145145Ferritin-like diiron

Sites

Metal binding181Iron 1
Metal binding511Iron 1
Metal binding511Iron 2
Metal binding521Iron (heme axial ligand); shared with dimeric partner By similarity
Metal binding541Iron 1
Metal binding941Iron 2
Metal binding1271Iron 1
Metal binding1271Iron 2
Metal binding1301Iron 2

Secondary structure

........... 159
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P63697 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 098B6D7392A9CD60

FASTA15918,341
        10         20         30         40         50         60 
MQGDPDVLRL LNEQLTSELT AINQYFLHSK MQDNWGFTEL AAHTRAESFD EMRHAEEITD 

        70         80         90        100        110        120 
RILLLDGLPN YQRIGSLRIG QTLREQFEAD LAIEYDVLNR LKPGIVMCRE KQDTTSAVLL 

       130        140        150 
EKIVADEEEH IDYLETQLEL MDKLGEELYS AQCVSRPPT 

« Hide

References

« Hide 'large scale' references
[1]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[3]"Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of bacterioferritin A from Mycobacterium tuberculosis."
Gupta V., Gupta R.K., Khare G., Salunke D.M., Tyagi A.K.
Acta Crystallogr. F 64:398-401(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION, HEME-BINDING.
Strain: ATCC 25618 / H37Rv.
[4]"Crystal structure of Bfr A from Mycobacterium tuberculosis: incorporation of selenomethionine results in cleavage and demetallation of haem."
Gupta V., Gupta R.K., Khare G., Salunke D.M., Tyagi A.K.
PLoS ONE 4:E8028-E8028(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.59 ANGSTROMS) IN COMPLEX WITH IRON IONS AND A DEGRADED PORPHYRIN DERIVATIVE, SUBUNIT.
Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000516 Genomic DNA. Translation: AAK46197.1.
AL123456 Genomic DNA. Translation: CCP44642.1.
PIRA70515.
RefSeqNP_216392.1. NC_000962.3.
NP_336383.1. NC_002755.2.
YP_006515276.1. NC_018143.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WTLX-ray2.59A/B/C/D/E/F1-159[»]
3QB9X-ray2.11A/B/C/D/E/F1-159[»]
3UOFX-ray2.90A/B/C/D/E/F1-159[»]
3UOIX-ray1.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x1-159[»]
ProteinModelPortalP63697.
SMRP63697. Positions 1-159.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING83332.Rv1876.

Proteomic databases

PaxDbP63697.
PRIDEP63697.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK46197; AAK46197; MT1925.
CCP44642; CCP44642; Rv1876.
GeneID13316667.
885767.
923656.
KEGGmtc:MT1925.
mtu:Rv1876.
mtv:RVBD_1876.
PATRIC18125997. VBIMycTub22151_2111.

Organism-specific databases

TubercuListRv1876.

Phylogenomic databases

eggNOGCOG2193.
HOGENOMHOG000262383.
KOK03594.
OMAEMKHADQ.
OrthoDBEOG6WDSKP.
ProtClustDBCLSK791451.

Enzyme and pathway databases

BioCycMTBCDC:MT1925-MONOMER.
MTBRV:RV1876-MONOMER.
ReactomeREACT_116125. Disease.

Family and domain databases

Gene3D1.20.1260.10. 1 hit.
InterProIPR002024. Bacterioferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PfamPF00210. Ferritin. 1 hit.
[Graphical view]
PIRSFPIRSF002560. Bacterioferritin. 1 hit.
PRINTSPR00601. BACFERRITIN.
SUPFAMSSF47240. SSF47240. 1 hit.
TIGRFAMsTIGR00754. bfr. 1 hit.
PROSITEPS00549. BACTERIOFERRITIN. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP63697.

Entry information

Entry nameBFR_MYCTU
AccessionPrimary (citable) accession number: P63697
Secondary accession number(s): L0T7Y6, O08465
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names