Reviewed, UniProtKB/Swiss-Prot P63628 (ASPG_MYCBO)

Names and origin

Protein names

Recommended name:
    Probable L-asparaginase
      Short name=L-ASNase
    EC=3.5.1.1
Alternative name(s):
    L-asparagine amidohydrolase

Gene names

Name:	ansA
Ordered Locus Names:	Mb1565c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the asparaginase 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Probable L-asparaginase		PRO_0000171085
Sites
	Active site	12	1	By similarity
	Active site	84	1	By similarity
	Active site	85	1	By similarity
	Active site	157	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63628-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: AEC46476F73FA340	FASTA	315	31,741
	10 20 30 40 50 60 MARLTVITTG GTISTTAGPD GVLRPTHCGA TLIAGLDMDS DIEVVDLMAL DSSKLTPADW 70 80 90 100 110 120 DRIGAAVQEA FRGGADGVVI THGTDTLEET ALWLDLTYAG SRPVVLTGAM LSADAPGADG 130 140 150 160 170 180 PANLRDALAV AADPAARDLG VLVSFGGRVL QPLGLHKVAN PDLCGFAGES LGFTSGGVRL 190 200 210 220 230 240 TRTKTRPYLG DLGAAVAPRV DIVAVYPGSD AVAMDACVAA GARAVVLEAL GSGNAGAAVI 250 260 270 280 290 300 EGVRRHCRDG SDPVVIAVST RVAGARVGAG YGPGHDLVEA GAVMVPRLPP SQARVLLMAA 310 LAANSPVADV IDRWG

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248339 Genomic DNA. Translation: CAD96232.1. Different initiation.
RefSeq	NP_855217.1.
3D structure databases
HSSP	HSSP built from PDB template 3PGA based on UniProtKB P10182.
ModBase	Search...
Genome annotation databases
GeneID	1092427.
GenomeReviews	Gene locus Mb1565c in contig BX248333_GR.
KEGG	mbo:Mb1565c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P63628.
Enzyme and pathway databases
BRENDA	3.5.1.1. 3091.
Family and domain databases
InterPro	IPR006034. Asp/Glutamnse. [Graphical view]
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR00139. ASNGLNASE.
ProDom	PD003221. Asp/Glutamnse. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG_MYCBO

Accession

Primary (citable) accession number: P63628
Secondary accession number(s): Q10759

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	September 22, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)