Reviewed, UniProtKB/Swiss-Prot P63615 (AROB_BRUME)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate synthase
    EC=4.2.3.4

Gene names

Name:	aroB
Ordered Locus Names:	BMEI0043

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. HAMAP MF_00110
Cofactor	NAD By similarity. HAMAP MF_00110 Divalent metal cations By similarity. HAMAP MF_00110
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. HAMAP MF_00110
Subunit structure	Monomer By similarity. HAMAP MF_00110
Subcellular location	Cytoplasm Probable HAMAP MF_00110.
Sequence similarities	Belongs to the dehydroquinate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	3-dehydroquinate synthase HAMAP MF_00110		PRO_0000140715

Sequences

	Sequence		Length	Mass (Da)
	P63615-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 940948AD815BEB00	FASTA	378	39,839
	10 20 30 40 50 60 MNAPTTVADS VTVPVSLGDR SYDILIGKGL VERAGEEVAK RLKGVRVAIV TDENVAAVHL 70 80 90 100 110 120 ERLQASFARA GIDSTPVIVA PGEKSKSFAT LETVTNAILA AKLERGDAVV ALGGGVVGDL 130 140 150 160 170 180 SGFVAGIVRR GMNFVQMPTS LLAQVDSSVG GKTGINTAHG KNLVGVFNQP QLVLADTQVL 190 200 210 220 230 240 DTLSPREFRA GYAEVAKYGL IDRPDFFAWL EANWQEVFSG GAARTKAIAE SCRSKAAVVA 250 260 270 280 290 300 RDERETGDRA LLNLGHTFGH ALESATGYDS SRLVHGEGVA IGMALAYRFS ARMNLAGIEA 310 320 330 340 350 360 AERVEAHLKA VGLPVSLAEV PGGLPPAEKL MDYIAQDKKV TRGTLTFILT HGIGQSFIAK 370 DVPPAAVLEF LKERLAIA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51225.1.
PIR	AF3257.
RefSeq	NP_538961.1.
3D structure databases
SMR	P63615. Positions 17-360.
ModBase	Search...
Genome annotation databases
GeneID	1195755.
GenomeReviews	Gene locus BMEI0043 in contig AE008917_GR.
KEGG	bme:BMEI0043.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG632303.
OMA	DKKMDAG.
PhylomeDB	P63615.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0043-MONOMER.
BRENDA	4.2.3.4. 277959.
Family and domain databases
HAMAP	MF_00110. DHQ_synthase. [Tree]
InterPro	IPR016303. DHQ_synth. IPR002658. DHQ_synth_AroB. IPR016037. DHQ_synth_AroB_sub. [Graphical view]
PANTHER	PTHR21090:SF1. DHQ_synth_AroB. 1 hit.
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001455. DHQ_synth. 1 hit.
TIGRFAMs	TIGR01357. aroB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROB_BRUME

Accession

Primary (citable) accession number: P63615
Secondary accession number(s): Q8YJN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)