P63588 (AROD_STRPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	SP_1377

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
apt	P63544	2	EBI-2207290,EBI-2207316
dnaJ	P95830	2	EBI-2207290,EBI-2207079
gatC	Q97SE5	2	EBI-2207290,EBI-2207053
groS	Q97NV3	2	EBI-2207290,EBI-2206949
pyrF	Q9ZHA7	2	EBI-2207290,EBI-2207109	From a different organism.
scpB	Q97NX6	2	EBI-2207290,EBI-2206697
uppS	Q97SR4	2	EBI-2207290,EBI-2206983

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000138816
Sites
	Active site	118	1	Proton acceptor By similarity
	Active site	143	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63588 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 1829E189C5BAEFB9	FASTA	225	25,719
	10 20 30 40 50 60 MKLIVSVMPR SLEEAQALDA TRYLDADIIE WRADYLPKEA ILQVAPAIFE KFAGRELVFT 70 80 90 100 110 120 LRTRSEGGEI DLSPEEYIHL IKEVAQLYQP DYIDFEYYSY KDVFEEMLDF PNLVLSYHNF 130 140 150 160 170 180 QETPENMMEI LSELTILNPK LVKVAVMAHT EQDVLDLMNY TRGFKTLNPE QEYVTISMGK 190 200 210 220 VGKVSRITAD VTGSSWSFAS LDEVSAPGQI SLASMKKIRE ILDEA

References

[1]

"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005672 Genomic DNA. Translation: AAK75475.1.
PIR	B95160.
RefSeq	NP_345835.1. NC_003028.3.
3D structure databases
ProteinModelPortal	P63588.
SMR	P63588. Positions 1-223.
ModBase	Search...
Protein-protein interaction databases
IntAct	P63588. 7 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000026565; EBSTRP00000025657; EBSTRG00000026565.
GeneID	931457.
GenomeReviews	Gene locus SP_1377 in contig AE005672_GR.
KEGG	spn:SP_1377.
PATRIC	19707159. VBIStrPne105772_1422.
TIGR	SP_1377.
Phylogenomic databases
GeneTree	EBGT00050000027890.
HOGENOM	HBG732926.
OMA	VARRTVI.
ProtClustDB	PRK02412.
Enzyme and pathway databases
BioCyc	SPNE170187-1:SP_1377-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_STRPN

Accession

Primary (citable) accession number: P63588
Secondary accession number(s): Q97Q54

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program