P63542 (APT_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenine phosphoribosyltransferase
Short name=APRT
EC=2.4.2.7

Gene names

Name:	apt
Ordered Locus Names:	BMEI0476

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. HAMAP MF_00004
Catalytic activity	AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00004
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP MF_00004
Subunit structure	Homodimer By similarity. HAMAP MF_00004
Subcellular location	Cytoplasm HAMAP MF_00004.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Sequence caution	The sequence AAL51657.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	adenine salvage Inferred from electronic annotation. Source: InterPro purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenine phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Adenine phosphoribosyltransferase HAMAP MF_00004		PRO_0000149364

Sequences

	Sequence		Length	Mass (Da)
	P63542 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 128ABF9D88C2A953	FASTA	181	19,614
	10 20 30 40 50 60 MESGFKVTLK DAIRTIPDYP KPGVQFRDVT TLMGNAQAFR RAVDELVYPY AGNRIDKVAG 70 80 90 100 110 120 IEARGFILGG AIAHQLSAGF VPIRKKGKLP RDTVRIAYSL EYGVDEMEMH RDAIEKGERV 130 140 150 160 170 180 VLVDDLIATG GTAEAAAKLL LQMGAEIVAA CFIIDLPDLG GRKKLEALGL PVRTLVAFEG D

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51657.1. Different initiation.
PIR	AF3311.
RefSeq	NP_539393.1. NC_003317.1.
3D structure databases
ProteinModelPortal	P63542.
SMR	P63542. Positions 8-181.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1196187.
GenomeReviews	Gene locus BMEI0476 in contig AE008917_GR.
KEGG	bme:BMEI0476.
PATRIC	17851050. VBIBruMel92729_0535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG703830.
OMA	GMEARGF.
PhylomeDB	P63542.
ProtClustDB	PRK02304.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0476-MONOMER.
Family and domain databases
HAMAP	MF_00004. Aden_phosphoribosyltr. [Tree]
InterPro	IPR005764. Ade_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
KO	K00759.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01090. Apt. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APT_BRUME

Accession

Primary (citable) accession number: P63542
Secondary accession number(s): Q8YIG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program