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Protein

Endonuclease III

Gene

nth

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi198Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi205Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi208Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi214Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Ordered Locus Names:BQ2027_MB3698C
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022171 – 245Endonuclease IIIAdd BLAST245

Structurei

3D structure databases

ProteinModelPortaliP63541.
SMRiP63541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 138HhHUniRule annotationAdd BLAST20

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252208.
KOiK10773.
OMAiKAKNPLC.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
HAMAPiMF_00942. Nth. 1 hit.
InterProiView protein in InterPro
IPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endonuclease3_FeS-loop_motif.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
PfamiView protein in Pfam
PF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
SMARTiView protein in SMART
SM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiView protein in PROSITE
PS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P63541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGRWSAETR LALVRRARRM NRALAQAFPH VYCELDFTTP LELAVATILS
60 70 80 90 100
AQSTDKRVNL TTPALFARYR TARDYAQADR TELESLIRPT GFYRNKAASL
110 120 130 140 150
IGLGQALVER FGGEVPATMD KLVTLPGVGR KTANVILGNA FGIPGITVDT
160 170 180 190 200
HFGRLVRRWR WTTAEDPVKV EQAVGELIER KEWTLLSHRV IFHGRRVCHA
210 220 230 240
RRPACGVCVL AKDCPSFGLG PTEPLLAAPL VQGPETDHLL ALAGL
Length:245
Mass (Da):27,030
Last modified:October 11, 2004 - v1
Checksum:i2B6D16195DD090DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA. Translation: SIU02326.1.
RefSeqiNP_857337.1. NC_002945.3.
WP_003419724.1. NC_002945.4.

Genome annotation databases

EnsemblBacteriaiCDO44969; CDO44969; Mb3698c.
KEGGimbo:Mb3698c.
PATRICifig|233413.5.peg.4049.

Similar proteinsi

Entry informationi

Entry nameiEND3_MYCBO
AccessioniPrimary (citable) accession number: P63541
Secondary accession number(s): A0A1R3Y5B1, O69642, X2BP01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: October 25, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families