Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P63541 (END3_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endonuclease III

EC=4.2.99.18
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene names
Name:nth
Ordered Locus Names:Mb3698c
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity. HAMAP-Rule MF_00942

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00942

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_00942

Sequence similarities

Belongs to the Nth/MutY family.

Contains 1 HhH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245Endonuclease III HAMAP-Rule MF_00942
PRO_0000102217

Regions

Domain119 – 13820HhH

Sites

Metal binding1981Iron-sulfur (4Fe-4S) By similarity
Metal binding2051Iron-sulfur (4Fe-4S) By similarity
Metal binding2081Iron-sulfur (4Fe-4S) By similarity
Metal binding2141Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P63541 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 2B6D16195DD090DE

FASTA24527,030
        10         20         30         40         50         60 
MPGRWSAETR LALVRRARRM NRALAQAFPH VYCELDFTTP LELAVATILS AQSTDKRVNL 

        70         80         90        100        110        120 
TTPALFARYR TARDYAQADR TELESLIRPT GFYRNKAASL IGLGQALVER FGGEVPATMD 

       130        140        150        160        170        180 
KLVTLPGVGR KTANVILGNA FGIPGITVDT HFGRLVRRWR WTTAEDPVKV EQAVGELIER 

       190        200        210        220        230        240 
KEWTLLSHRV IFHGRRVCHA RRPACGVCVL AKDCPSFGLG PTEPLLAAPL VQGPETDHLL 


ALAGL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248333 Genomic DNA. Translation: CDO44969.1.
RefSeqNP_857337.1. NC_002945.3.

3D structure databases

ProteinModelPortalP63541.
SMRP63541. Positions 24-241.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb3698c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD95884; CAD95884; Mb3698c.
GeneID1093265.
KEGGmbo:Mb3698c.
PATRIC18009846. VBIMycBov88188_4049.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0177.
HOGENOMHOG000252208.
KOK10773.
OMANCSIDIL.
OrthoDBEOG6H4KC5.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPMF_00942. Nth.
InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFPIRSF001435. Nth. 1 hit.
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. SSF48150. 1 hit.
TIGRFAMsTIGR01083. nth. 1 hit.
PROSITEPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND3_MYCBO
AccessionPrimary (citable) accession number: P63541
Secondary accession number(s): O69642, X2BP01
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families