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P63540 (END3_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease III
EC=4.2.99.18
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene names
Name:nth
Ordered Locus Names:Rv3674c, MT3775
ORF Names:MTV025.022c
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site By similarity.

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand By similarity.

Sequence similarities

Belongs to the Nth/MutY family.

Sequence caution

The sequence AAK48142.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245Endonuclease III
PRO_0000102219

Sites

Metal binding1981Iron-sulfur (4Fe-4S) By similarity
Metal binding2051Iron-sulfur (4Fe-4S) By similarity
Metal binding2081Iron-sulfur (4Fe-4S) By similarity
Metal binding2141Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P63540 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 2B6D16195DD090DE

FASTA24527,030
        10         20         30         40         50         60 
MPGRWSAETR LALVRRARRM NRALAQAFPH VYCELDFTTP LELAVATILS AQSTDKRVNL 

        70         80         90        100        110        120 
TTPALFARYR TARDYAQADR TELESLIRPT GFYRNKAASL IGLGQALVER FGGEVPATMD 

       130        140        150        160        170        180 
KLVTLPGVGR KTANVILGNA FGIPGITVDT HFGRLVRRWR WTTAEDPVKV EQAVGELIER 

       190        200        210        220        230        240 
KEWTLLSHRV IFHGRRVCHA RRPACGVCVL AKDCPSFGLG PTEPLLAAPL VQGPETDHLL 


ALAGL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842583 Genomic DNA. Translation: CAA17996.2.
AE000516 Genomic DNA. Translation: AAK48142.1. Different initiation.
PIRC70790.
RefSeqNP_218191.2. NC_000962.2.
NP_338328.1. NC_002755.2.

3D structure databases

ProteinModelPortalP63540.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000000148; EBMYCP00000000148; EBMYCG00000000148.
EBMYCT00000071122; EBMYCP00000069181; EBMYCG00000071117.
GeneID885058.
926474.
GenomeReviewsGene locus MT3775 in contig AE000516_GR.
Gene locus Rv3674c in contig AL123456_GR.
KEGGmtc:MT3775.
mtu:Rv3674c.
PATRIC18130064. VBIMycTub22151_4125.
TIGRMT3775.

Organism-specific databases

TubercuListRv3674c.

Phylogenomic databases

GeneTreeEBGT00050000016637.
HOGENOMHBG464473.
OMAEVPQTRE.
PhylomeDBP63540.
ProtClustDBCLSK799395.

Family and domain databases

InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III_CS2.
IPR005759. Endonuclease-III_Nth.
IPR004035. Endouclease-III_FeS-bd_BS.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
[Graphical view]
Gene3DG3DSA:1.10.340.30. DNA_glycosylase. 1 hit.
G3DSA:1.10.1670.10. HTH_base_excis_C. 1 hit.
KOK10773.
PfamPF10576. EndIII_4Fe-2S. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. DNA_glycsylse. 1 hit.
TIGRFAMsTIGR01083. Nth. 1 hit.
PROSITEPS00764. ENDONUCLEASE_III_1. 1 hit.
PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND3_MYCTU
AccessionPrimary (citable) accession number: P63540
Secondary accession number(s): O69642
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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