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Protein

Probable endonuclease 4

Gene

nfo

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Zinc 1UniRule annotation1
Metal bindingi109Zinc 1UniRule annotation1
Metal bindingi144Zinc 1UniRule annotation1
Metal bindingi144Zinc 2UniRule annotation1
Metal bindingi178Zinc 2UniRule annotation1
Metal bindingi181Zinc 3UniRule annotation1
Metal bindingi213Zinc 2UniRule annotation1
Metal bindingi226Zinc 3UniRule annotation1
Metal bindingi228Zinc 3UniRule annotation1
Metal bindingi258Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:SAV1557
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908701 – 296Probable endonuclease 4Add BLAST296

Proteomic databases

PaxDbiP63537.
PRIDEiP63537.

2D gel databases

World-2DPAGEi0002:P63537.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1557.

Structurei

3D structure databases

ProteinModelPortaliP63537.
SMRiP63537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224895.
KOiK01151.
OMAiHPGSHLK.
PhylomeDBiP63537.

Family and domain databases

CDDicd00019. AP2Ec. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiView protein in InterPro
IPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR036237. Xyl_isomerase-like_sf.
IPR013022. Xyl_isomerase-like_TIM-brl.
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiView protein in Pfam
PF01261. AP_endonuc_2. 1 hit.
SMARTiView protein in SMART
SM00518. AP2Ec. 1 hit.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiView protein in PROSITE
PS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.

Sequencei

Sequence statusi: Complete.

P63537-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLGSHVSMS GKKMLEGSAI EAYEYGETTF MIYTGAPQNT RRKSIEDLNI
60 70 80 90 100
TKGHEVMEKY GLSNIVVHAP YIINIANTTK PETFNLGVDF LQQEIERTQA
110 120 130 140 150
IGAKDIVLHP GAHVGAGVDA GINKIIEGLN EVLTNDNNVR IALETMAGKG
160 170 180 190 200
TEIGRSFEEL ARIIDGVHNN ERLSVCFDTC HTHDAGYNVK EDFDGVLNEF
210 220 230 240 250
DKIIGVDRIK VVHVNDSKND RGAQKDRHEN IGFGYIGFDA LNYIVHHDSF
260 270 280 290
KDIPKILETP YVGEDKKNKK PPYKLEIEML KQQQFDPELK NKVMQQ
Length:296
Mass (Da):33,185
Last modified:October 11, 2004 - v1
Checksum:i6B32D9961F9BEC3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57719.1.
RefSeqiWP_000924220.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57719; BAB57719; SAV1557.
KEGGisav:SAV1557.

Similar proteinsi

Entry informationi

Entry nameiEND4_STAAM
AccessioniPrimary (citable) accession number: P63537
Secondary accession number(s): Q99TT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: October 25, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families