P63522 (LCFA_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain-fatty-acid--CoA ligase
EC=6.2.1.3

Alternative name(s):
Long-chain acyl-CoA synthetase

Gene names

Name:	fadD
Ordered Locus Names:	STY1948, t1059

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Subunit structure	Homodimer Probable.
Subcellular location	Membrane; Peripheral membrane protein Potential. Note: Partially membrane-associated Potential.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain fatty acid-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Long-chain-fatty-acid--CoA ligase		PRO_0000193128
Regions
	Nucleotide binding	213 – 224	12	ATP Probable

Sequences

	Sequence		Length	Mass (Da)
	P63522 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 4000B2948FE38D15	FASTA	561	62,223
	10 20 30 40 50 60 MKKVWLNRYP ADVPAEINPD RYQSLVELFE HAATRYADQP AFVNMGEVMT FRKLEERSRA 70 80 90 100 110 120 FAAYLQQGLG LKKGDRVALM MPNLLQYPVA LFGILRAGMI VVNVNPLYTP RELEHQLNDS 130 140 150 160 170 180 GAAAIIIVSN FAHTLEKVVE KTSVQHVILT RMGDQLSTAK GTVVNFVVKY IKRLVPKYHL 190 200 210 220 230 240 PDAISFRSAL QHGYRMQYVK PEVVAEDLAF LQYTGGTTGV AKGAMLTHRN MLANLEQVKA 250 260 270 280 290 300 TYGPLLHPGK ELVVTALPLY HIFALTMNCL LFIELGGQNL LITNPRDIPG LVKELAKYPF 310 320 330 340 350 360 TAMTGVNTLF NALLNNKEFQ QLDFSSLHLS AGGGMPVQNV VAERWVKLTG QYLLEGYGLT 370 380 390 400 410 420 ECAPLVSVNP HDIDYHSGSI GLPVPSTEAK LVDDDDNEVA PGEAGELCVK GPQVMLGYWQ 430 440 450 460 470 480 RPDATDEIIK DGWLHTGDIA VMDEDGFLRI VDRKKDMILV SGFNVYPNEI EDVVMQHSGV 490 500 510 520 530 540 QEVAAVGVPS GSSGEAVKLF VVKKDPALTD DALITFCRRH LTGYKVPKQV EFREELPKSN 550 560 VGKILRRELR DEARGKVDNK A

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627272 Genomic DNA. Translation: CAD05501.1. AE014613 Genomic DNA. Translation: AAO68725.1.
RefSeq	NP_456325.1. NC_003198.1. NP_804876.1. NC_004631.1.
3D structure databases
ProteinModelPortal	P63522.
ModBase	Search...
Proteomic databases
PRIDE	P63522.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1070404. 1248301.
GenomeReviews	Gene locus t1059 in contig AE014613_GR. Gene locus STY1948 in contig AL513382_GR.
KEGG	stt:t1059. sty:STY1948.
PATRIC	18541813. VBISalEnt120419_1965.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	MPVQQAV.
ProtClustDB	PRK08974.
Enzyme and pathway databases
BioCyc	SENT209261:T1059-MONOMER. SENT220341:STY1948-MONOMER.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01897.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCFA_SALTI

Accession

Primary (citable) accession number: P63522
Secondary accession number(s): Q8XGG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program