Reviewed, UniProtKB/Swiss-Prot P63503 (CBL_MYCBO)

Names and origin

Protein names

Recommended name:
    Putative cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase

Gene names

Ordered Locus Names:

Mb2316

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/patB cystathionine beta-lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Putative cystathionine beta-lyase		PRO_0000163851
Amino acid modifications
	Modified residue	237	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63503-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: AA6296F845E2622F	FASTA	407	44,283
	10 20 30 40 50 60 MIPNPLEELT LEQLRSQRTS MKWRAHPADV LPLWVAEMDV KLPPTVADAL RRAIDDGDTG 70 80 90 100 110 120 YPYGTEYAEA VREFACQRWQ WHDLEVSRTA IVPDVMLGIV EVLRLITDRG DPVIVNSPVY 130 140 150 160 170 180 APFYAFVSHD GRRVIPAPLR GDGRIDLDAL QEAFSSARAS SGSSGNVAYL LCNPHNPTGS 190 200 210 220 230 240 VHTADELRGI AERAQRFGVR VVSDEIHAPL IPSGARFTPY LSVPGAENAF ALMSASKAWN 250 260 270 280 290 300 LGGLKAALAI AGREAAADLA RMPEEVGHGP SHLGVIAHTA AFRTGGNWLD ALLRGLDHNR 310 320 330 340 350 360 TLLGALVDEH LPGVQYRWPQ GTYLAWLDCR ELGFDDAASD EMTEGLAVVS DLSGPARWFL 370 380 390 400 DHARVALSSG HVFGIGGAGH VRINFATSRA ILIEAVSRMS RSLLERR

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248342 Genomic DNA. Translation: CAD97177.1.
RefSeq	NP_855965.1.
3D structure databases
HSSP	HSSP built from PDB template 1D2F based on UniProtKB P23256.
ModBase	Search...
Genome annotation databases
GeneID	1090409.
GenomeReviews	Gene locus Mb2316 in contig BX248333_GR.
KEGG	mbo:Mb2316.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P63503.
OMA	LANPLRE.
Family and domain databases
InterPro	IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PROSITE	PS00599. AA_TRANSFER_CLASS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBL_MYCBO

Accession

Primary (citable) accession number: P63503
Secondary accession number(s): Q50672

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)