P63499 (AAT_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable aspartate aminotransferase
Short name=AspAT
EC=2.6.1.1

Alternative name(s):
Transaminase A

Gene names

Name:	aspC
Ordered Locus Names:	Mb0344c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: InterPro L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC L-phenylalanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Probable aspartate aminotransferase		PRO_0000123844
Amino acid modifications
	Modified residue	265	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63499 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 7B4944351190071A	FASTA	429	47,350
	10 20 30 40 50 60 MDNDGTIVDV TTHQLPWHTA SHQRQRAFAQ SAKLQDVLYE IRGPVHQHAA RLEAEGHRIL 70 80 90 100 110 120 KLNIGNPAPF GFEAPDVIMR DIIQALPYAQ GYSDSQGILS ARRAVVTRYE LVPGFPRFDV 130 140 150 160 170 180 DDVYLGNGVS ELITMTLQAL LDNGDQVLIP SPDYPLWTAS TSLAGGTPVH YLCDETQGWQ 190 200 210 220 230 240 PDIADLESKI TERTKALVVI NPNNPTGAVY SCEILTQMVD LARKHQLLLL ADEIYDKILY 250 260 270 280 290 300 DDAKHISLAS IAPDMLCLTF NGLSKAYRVA GYRAGWLAIT GPKEHASSFI EGIGLLANMR 310 320 330 340 350 360 LCPNVPAQHA IQVALGGHQS IEDLVLPGGR LLEQRDIAWT KLNEIPGVSC VKPAGALYAF 370 380 390 400 410 420 PRLDPEVYDI DDDEQLVLDL LLSEKILVTQ GTGFNWPAPD HLRLVTLPWS RDLAAAIERL GNFLVSYRQ

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248335 Genomic DNA. Translation: CAD93208.1.
RefSeq	NP_854008.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P63499.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000014688; EBMYCP00000014523; EBMYCG00000014685.
GeneID	1091217.
GenomeReviews	Gene locus Mb0344c in contig BX248333_GR.
KEGG	mbo:Mb0344c.
PATRIC	18002410. VBIMycBov88188_0374.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000031717.
HOGENOM	HBG645860.
OMA	ELIVPSG.
ProtClustDB	PRK09265.
Enzyme and pathway databases
BioCyc	MBOV233413:MB0344C-MONOMER.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K14260.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT_MYCBO

Accession

Primary (citable) accession number: P63499
Secondary accession number(s): O33267

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program