P63498 (AAT_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable aspartate aminotransferase
Short name=AspAT
EC=2.6.1.1

Alternative name(s):
Transaminase A

Gene names

Name:	aspC
Ordered Locus Names:	Rv0337c, MT0351
ORF Names:	MTCY279.04c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biosynthetic process Inferred from electronic annotation. Source: InterPro growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 14532352PubMed 15525680. Source: MTBBASE
Molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC L-phenylalanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Probable aspartate aminotransferase		PRO_0000123843
Amino acid modifications
	Modified residue	265	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63498 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 7B4944351190071A	FASTA	429	47,350
	10 20 30 40 50 60 MDNDGTIVDV TTHQLPWHTA SHQRQRAFAQ SAKLQDVLYE IRGPVHQHAA RLEAEGHRIL 70 80 90 100 110 120 KLNIGNPAPF GFEAPDVIMR DIIQALPYAQ GYSDSQGILS ARRAVVTRYE LVPGFPRFDV 130 140 150 160 170 180 DDVYLGNGVS ELITMTLQAL LDNGDQVLIP SPDYPLWTAS TSLAGGTPVH YLCDETQGWQ 190 200 210 220 230 240 PDIADLESKI TERTKALVVI NPNNPTGAVY SCEILTQMVD LARKHQLLLL ADEIYDKILY 250 260 270 280 290 300 DDAKHISLAS IAPDMLCLTF NGLSKAYRVA GYRAGWLAIT GPKEHASSFI EGIGLLANMR 310 320 330 340 350 360 LCPNVPAQHA IQVALGGHQS IEDLVLPGGR LLEQRDIAWT KLNEIPGVSC VKPAGALYAF 370 380 390 400 410 420 PRLDPEVYDI DDDEQLVLDL LLSEKILVTQ GTGFNWPAPD HLRLVTLPWS RDLAAAIERL GNFLVSYRQ

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842573 Genomic DNA. Translation: CAB10792.1. AE000516 Genomic DNA. Translation: AAK44574.1. AL123456 Genomic DNA. Translation: CCP43067.1.
PIR	H70506.
RefSeq	NP_214851.1. NC_000962.3. NP_334760.1. NC_002755.2. YP_006513663.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P63498.
SMR	P63498. Positions 49-424.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv0337c.
Proteomic databases
PRIDE	P63498.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK44574; AAK44574; MT0351.
GeneID	13318204. 886522. 923493.
KEGG	mtc:MT0351. mtu:Rv0337c. mtv:RVBD_0337c.
PATRIC	18122484. VBIMycTub22151_0376.
Organism-specific databases
TubercuList	Rv0337c.
Phylogenomic databases
eggNOG	COG0436.
HOGENOM	HOG000223042.
KO	K14260.
OMA	YQARDMR.
ProtClustDB	PRK09265.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT_MYCTU

Accession

Primary (citable) accession number: P63498
Secondary accession number(s): L0T6D2, O33267

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program