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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Charge relay systemUniRule annotation1
Active sitei154Charge relay systemUniRule annotation1
Active sitei178Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:SAV1900
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001051991 – 485Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST485

Proteomic databases

PaxDbiP63488.
PRIDEiP63488.

2D gel databases

World-2DPAGE0002:P63488.

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi158878.SAV1900.

Structurei

Secondary structure

1485
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Helixi8 – 16Combined sources9
Helixi22 – 40Combined sources19
Beta strandi43 – 46Combined sources4
Helixi48 – 63Combined sources16
Turni70 – 73Combined sources4
Beta strandi75 – 79Combined sources5
Helixi94 – 96Combined sources3
Helixi106 – 113Combined sources8
Beta strandi117 – 122Combined sources6
Helixi126 – 128Combined sources3
Beta strandi131 – 133Combined sources3
Beta strandi153 – 155Combined sources3
Helixi156 – 163Combined sources8
Beta strandi168 – 173Combined sources6
Beta strandi175 – 177Combined sources3
Helixi180 – 185Combined sources6
Beta strandi189 – 192Combined sources4
Turni208 – 210Combined sources3
Beta strandi213 – 219Combined sources7
Helixi220 – 230Combined sources11
Turni250 – 253Combined sources4
Beta strandi260 – 264Combined sources5
Helixi265 – 268Combined sources4
Beta strandi269 – 272Combined sources4
Helixi274 – 289Combined sources16
Beta strandi293 – 297Combined sources5
Helixi302 – 304Combined sources3
Helixi305 – 320Combined sources16
Turni321 – 323Combined sources3
Beta strandi325 – 330Combined sources6
Helixi339 – 350Combined sources12
Helixi353 – 366Combined sources14
Turni368 – 374Combined sources7
Helixi375 – 393Combined sources19
Beta strandi397 – 407Combined sources11
Turni411 – 413Combined sources3
Helixi418 – 422Combined sources5
Helixi423 – 425Combined sources3
Turni426 – 428Combined sources3
Helixi429 – 434Combined sources6
Beta strandi438 – 446Combined sources9
Beta strandi449 – 456Combined sources8
Helixi462 – 475Combined sources14
Turni479 – 481Combined sources3
Helixi482 – 484Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DF4X-ray3.20A1-485[»]
2DQNX-ray2.55A1-485[»]
2F2AX-ray2.30A1-485[»]
2G5HX-ray2.50A1-485[»]
2G5IX-ray3.35A1-485[»]
3IP4X-ray1.90A1-485[»]
ProteinModelPortaliP63488.
SMRiP63488.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63488.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
PhylomeDBiP63488.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRYESVEN LLTLIKDKKI KPSDVVKDIY DAIEETDPTI KSFLALDKEN
60 70 80 90 100
AIKKAQELDE LQAKDQMDGK LFGIPMGIKD NIITNGLETT CASKMLEGFV
110 120 130 140 150
PIYESTVMEK LHKENAVLIG KLNMDEFAMG GSTETSYFKK TVNPFDHKAV
160 170 180 190 200
PGGSSGGSAA AVAAGLVPLS LGSDTGGSIR QPAAYCGVVG MKPTYGRVSR
210 220 230 240 250
FGLVAFASSL DQIGPLTRNV KDNAIVLEAI SGADVNDSTS APVDDVDFTS
260 270 280 290 300
EIGKDIKGLK VALPKEYLGE GVADDVKEAV QNAVETLKSL GAVVEEVSLP
310 320 330 340 350
NTKFGIPSYY VIASSEASSN LSRFDGIRYG YHSKEAHSLE ELYKMSRSEG
360 370 380 390 400
FGKEVKRRIF LGTFALSSGY YDAYYKKSQK VRTLIKNDFD KVFENYDVVV
410 420 430 440 450
GPTAPTTAFN LGEEIDDPLT MYANDLLTTP VNLAGLPGIS VPCGQSNGRP
460 470 480
IGLQFIGKPF DEKTLYRVAY QYETQYNLHD VYEKL
Length:485
Mass (Da):52,801
Last modified:October 11, 2004 - v1
Checksum:i52F78A35E4863965
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58062.1.
RefSeqiWP_000027919.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB58062; BAB58062; SAV1900.
KEGGisav:SAV1900.
PATRICi19564654. VBIStaAur52173_1967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58062.1.
RefSeqiWP_000027919.1. NC_002758.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DF4X-ray3.20A1-485[»]
2DQNX-ray2.55A1-485[»]
2F2AX-ray2.30A1-485[»]
2G5HX-ray2.50A1-485[»]
2G5IX-ray3.35A1-485[»]
3IP4X-ray1.90A1-485[»]
ProteinModelPortaliP63488.
SMRiP63488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1900.

2D gel databases

World-2DPAGE0002:P63488.

Proteomic databases

PaxDbiP63488.
PRIDEiP63488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB58062; BAB58062; SAV1900.
KEGGisav:SAV1900.
PATRICi19564654. VBIStaAur52173_1967.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
PhylomeDBiP63488.

Miscellaneous databases

EvolutionaryTraceiP63488.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_STAAM
AccessioniPrimary (citable) accession number: P63488
Secondary accession number(s): Q99SY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.