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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Charge relay systemUniRule annotation1
Active sitei154Charge relay systemUniRule annotation1
Active sitei178Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:SAV1900
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001051991 – 485Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST485

Proteomic databases

PaxDbiP63488

2D gel databases

World-2DPAGEi0002:P63488

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi158878.SAV1900

Structurei

Secondary structure

1485
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Helixi8 – 16Combined sources9
Helixi22 – 40Combined sources19
Beta strandi43 – 46Combined sources4
Helixi48 – 63Combined sources16
Turni70 – 73Combined sources4
Beta strandi75 – 79Combined sources5
Helixi94 – 96Combined sources3
Helixi106 – 113Combined sources8
Beta strandi117 – 122Combined sources6
Helixi126 – 128Combined sources3
Beta strandi131 – 133Combined sources3
Beta strandi153 – 155Combined sources3
Helixi156 – 163Combined sources8
Beta strandi168 – 173Combined sources6
Beta strandi175 – 177Combined sources3
Helixi180 – 185Combined sources6
Beta strandi189 – 192Combined sources4
Turni208 – 210Combined sources3
Beta strandi213 – 219Combined sources7
Helixi220 – 230Combined sources11
Turni250 – 253Combined sources4
Beta strandi260 – 264Combined sources5
Helixi265 – 268Combined sources4
Beta strandi269 – 272Combined sources4
Helixi274 – 289Combined sources16
Beta strandi293 – 297Combined sources5
Helixi302 – 304Combined sources3
Helixi305 – 320Combined sources16
Turni321 – 323Combined sources3
Beta strandi325 – 330Combined sources6
Helixi339 – 350Combined sources12
Helixi353 – 366Combined sources14
Turni368 – 374Combined sources7
Helixi375 – 393Combined sources19
Beta strandi397 – 407Combined sources11
Turni411 – 413Combined sources3
Helixi418 – 422Combined sources5
Helixi423 – 425Combined sources3
Turni426 – 428Combined sources3
Helixi429 – 434Combined sources6
Beta strandi438 – 446Combined sources9
Beta strandi449 – 456Combined sources8
Helixi462 – 475Combined sources14
Turni479 – 481Combined sources3
Helixi482 – 484Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DF4X-ray3.20A1-485[»]
2DQNX-ray2.55A1-485[»]
2F2AX-ray2.30A1-485[»]
2G5HX-ray2.50A1-485[»]
2G5IX-ray3.35A1-485[»]
3IP4X-ray1.90A1-485[»]
ProteinModelPortaliP63488
SMRiP63488
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63488

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P Bacteria
COG0154 LUCA
HOGENOMiHOG000116699
KOiK02433
OMAiPITQNVE
PhylomeDBiP63488

Family and domain databases

Gene3Di3.90.1300.10, 1 hit
HAMAPiMF_00120 GatA, 1 hit
InterProiView protein in InterPro
IPR000120 Amidase
IPR020556 Amidase_CS
IPR023631 Amidase_dom
IPR036928 AS_sf
IPR004412 GatA
PANTHERiPTHR11895 PTHR11895, 1 hit
PfamiView protein in Pfam
PF01425 Amidase, 1 hit
SUPFAMiSSF75304 SSF75304, 1 hit
TIGRFAMsiTIGR00132 gatA, 1 hit
PROSITEiView protein in PROSITE
PS00571 AMIDASES, 1 hit

Sequencei

Sequence statusi: Complete.

P63488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRYESVEN LLTLIKDKKI KPSDVVKDIY DAIEETDPTI KSFLALDKEN
60 70 80 90 100
AIKKAQELDE LQAKDQMDGK LFGIPMGIKD NIITNGLETT CASKMLEGFV
110 120 130 140 150
PIYESTVMEK LHKENAVLIG KLNMDEFAMG GSTETSYFKK TVNPFDHKAV
160 170 180 190 200
PGGSSGGSAA AVAAGLVPLS LGSDTGGSIR QPAAYCGVVG MKPTYGRVSR
210 220 230 240 250
FGLVAFASSL DQIGPLTRNV KDNAIVLEAI SGADVNDSTS APVDDVDFTS
260 270 280 290 300
EIGKDIKGLK VALPKEYLGE GVADDVKEAV QNAVETLKSL GAVVEEVSLP
310 320 330 340 350
NTKFGIPSYY VIASSEASSN LSRFDGIRYG YHSKEAHSLE ELYKMSRSEG
360 370 380 390 400
FGKEVKRRIF LGTFALSSGY YDAYYKKSQK VRTLIKNDFD KVFENYDVVV
410 420 430 440 450
GPTAPTTAFN LGEEIDDPLT MYANDLLTTP VNLAGLPGIS VPCGQSNGRP
460 470 480
IGLQFIGKPF DEKTLYRVAY QYETQYNLHD VYEKL
Length:485
Mass (Da):52,801
Last modified:October 11, 2004 - v1
Checksum:i52F78A35E4863965
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA Translation: BAB58062.1
RefSeqiWP_000027919.1, NC_002758.2

Genome annotation databases

EnsemblBacteriaiBAB58062; BAB58062; SAV1900
KEGGisav:SAV1900

Entry informationi

Entry nameiGATA_STAAM
AccessioniPrimary (citable) accession number: P63488
Secondary accession number(s): Q99SY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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