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P63488 (GATA_STAAM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-
Gene names
Name:gatA
Ordered Locus Names:SAV1900
OrganismStaphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]
Taxonomic identifier158878 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP MF_00120

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP MF_00120

Subunit structure

Heterotrimer of A, B and C subunits By similarity. HAMAP MF_00120

Sequence similarities

Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

carbon-nitrogen ligase activity, with glutamine as amido-N-donor

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP MF_00120
PRO_0000105199

Sites

Active site791Charge relay system By similarity
Active site1541Charge relay system By similarity
Active site1781Acyl-ester intermediate By similarity

Secondary structure

1 ............................................................................... 485
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P63488 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 52F78A35E4863965

FASTA48552,801
        10         20         30         40         50         60 
MSIRYESVEN LLTLIKDKKI KPSDVVKDIY DAIEETDPTI KSFLALDKEN AIKKAQELDE 

        70         80         90        100        110        120 
LQAKDQMDGK LFGIPMGIKD NIITNGLETT CASKMLEGFV PIYESTVMEK LHKENAVLIG 

       130        140        150        160        170        180 
KLNMDEFAMG GSTETSYFKK TVNPFDHKAV PGGSSGGSAA AVAAGLVPLS LGSDTGGSIR 

       190        200        210        220        230        240 
QPAAYCGVVG MKPTYGRVSR FGLVAFASSL DQIGPLTRNV KDNAIVLEAI SGADVNDSTS 

       250        260        270        280        290        300 
APVDDVDFTS EIGKDIKGLK VALPKEYLGE GVADDVKEAV QNAVETLKSL GAVVEEVSLP 

       310        320        330        340        350        360 
NTKFGIPSYY VIASSEASSN LSRFDGIRYG YHSKEAHSLE ELYKMSRSEG FGKEVKRRIF 

       370        380        390        400        410        420 
LGTFALSSGY YDAYYKKSQK VRTLIKNDFD KVFENYDVVV GPTAPTTAFN LGEEIDDPLT 

       430        440        450        460        470        480 
MYANDLLTTP VNLAGLPGIS VPCGQSNGRP IGLQFIGKPF DEKTLYRVAY QYETQYNLHD 


VYEKL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000017 Genomic DNA. Translation: BAB58062.1.
RefSeqNP_372424.1. NC_002758.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DF4X-ray3.20A1-485[»]
2DQNX-ray2.55A1-485[»]
2F2AX-ray2.30A1-485[»]
2G5HX-ray2.50A1-485[»]
2G5IX-ray3.35A1-485[»]
3IP4X-ray1.90A1-485[»]
ProteinModelPortalP63488.
SMRP63488. Positions 1-485.
ModBaseSearch...

Protein-protein interaction databases

STRINGP63488.

2D gel databases

World-2DPAGE0002:P63488.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000006792; EBSTAP00000006610; EBSTAG00000006791.
GeneID1121913.
GenomeReviewsGene locus SAV1900 in contig BA000017_GR.
KEGGsav:SAV1900.
PATRIC19564654. VBIStaAur52173_1967.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0154.
GeneTreeEBGT00050000024611.
HOGENOMHBG481888.
OMATESSCYG.
PhylomeDBP63488.
ProtClustDBPRK00012.

Enzyme and pathway databases

BioCycSAUR158878:SAV1900-MONOMER.

Family and domain databases

HAMAPMF_00120. GatA.
[Tree]
InterProIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
Gene3DG3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit.
KOK02433.
PANTHERPTHR11895. Amidase. 1 hit.
PfamPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMSSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMsTIGR00132. GatA. 1 hit.
PROSITEPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATA_STAAM
AccessionPrimary (citable) accession number: P63488
Secondary accession number(s): Q99SY6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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