P63465 (ACPS_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holo-[acyl-carrier-protein] synthase
Short name=Holo-ACP synthase
EC=2.7.8.7

Alternative name(s):
4'-phosphopantetheinyl transferase AcpS

Gene names

Name:	acpS
Ordered Locus Names:	BR0659, BS1330_I0655

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein By similarity. HAMAP MF_00101
Catalytic activity	CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. HAMAP MF_00101
Cofactor	Magnesium By similarity. HAMAP MF_00101
Subcellular location	Cytoplasm By similarity HAMAP MF_00101.
Sequence similarities	Belongs to the P-Pant transferase superfamily. AcpS family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Holo-[acyl-carrier-protein] synthase HAMAP MF_00101		PRO_0000175620
Sites
	Metal binding	8	1	Magnesium By similarity
	Metal binding	57	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63465 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 95A7F7D426A07BDA	FASTA	134	14,645
	10 20 30 40 50 60 MIVGIGSDLI DIRRVEKTLE RHGSRFRDRV FTEIEQRKSE GRKQRAASYA KRFAAKEACA 70 80 90 100 110 120 KALGTGIAEG VFWRDMGVVN TPSGKPTMHL TGGAAKQLQK LLPAGTNAAI HLTITDDFPL 130 AQAFVIIEAL PVLE

References

[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29588.1. CP002997 Genomic DNA. Translation: AEM18005.1.
RefSeq	NP_697673.1. NC_004310.3.
3D structure databases
ProteinModelPortal	P63465.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1166322.
GenomeReviews	Gene locus BR0659 in contig AE014291_GR.
KEGG	bms:BR0659.
PATRIC	17789611. VBIBruSui107850_0672.
TIGR	BR0659.
Phylogenomic databases
HOGENOM	HBG734479.
OMA	FWKDMGV.
PhylomeDB	P63465.
ProtClustDB	PRK00070.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0659-MONOMER.
Family and domain databases
HAMAP	MF_00101. AcpS. [Tree]
InterPro	IPR008278. 4-PPantetheinyl_Trfase. IPR002582. ACPS. IPR004568. PPantethiene-prot_Trfase. [Graphical view]
Gene3D	G3DSA:3.90.470.20. G3DSA:3.90.470.20. 1 hit.
KO	K00997.
Pfam	PF01648. ACPS. 1 hit. [Graphical view]
ProDom	PD004282. PPantethiene-prot_Trfase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56214. 4-PPT_transf. 1 hit.
TIGRFAMs	TIGR00516. AcpS. 1 hit. TIGR00556. Pantethn_trn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACPS_BRUSU

Accession

Primary (citable) accession number: P63465
Secondary accession number(s): G0K800, Q8YG72

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program