P63458 (FABD_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malonyl CoA-acyl carrier protein transacylase
Short name=MCT
EC=2.3.1.39

Gene names

Name:	fabD
Ordered Locus Names:	Rv2243, MT2303
ORF Names:	MTCY427.24

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].
Pathway	Lipid metabolism; fatty acid biosynthesis.
Post-translational modification	Pupylated at Lys-173 by the prokaryotic ubiquitin-like protein Pup, which leads to its degradation by the proteasome. Ref.4 Ref.6
Miscellaneous	Was identified as a natural substrate of the M.tuberculosis proteasome. Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the FabD family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Molecular function	Acyltransferase Transferase
PTM	Isopeptide bond Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from direct assay PubMed 17604051. Source: MTBBASE protein homooligomerization Inferred from physical interaction PubMed 16213523. Source: MTBBASE
Cellular_component	fatty acid synthase complex Inferred from direct assay PubMed 16213523. Source: MTBBASE
Molecular_function	[acyl-carrier-protein] S-malonyltransferase activity Inferred from direct assay PubMed 11373295PubMed 17604051. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 302

302

Malonyl CoA-acyl carrier protein transacylase

PRO_0000194218

Regions

Compositional bias

206 – 210

5

Poly-Ala

Compositional bias

233 – 236

4

Poly-Ala

Sites

Active site

91

1

By similarity

Active site

194

1

By similarity

Amino acid modifications

Cross-link

173

Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Experimental info

Mutagenesis

173

1

K → A: Nearly abolishes pupylation and dramatically stabilizes this proteasome substrate in wild-type mycobacteria. Ref.4

Secondary structure

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302

Helix Strand Turn

Details...

Beta strand

1 – 6

6

Turn

14 – 22

9

Helix

26 – 36

11

Helix

41 – 46

6

Helix

50 – 53

4

Helix

56 – 76

21

Turn

77 – 82

6

Beta strand

85 – 89

5

Helix

93 – 100

8

Helix

106 – 124

19

Beta strand

129 – 137

9

Helix

139 – 148

10

Beta strand

152 – 158

7

Beta strand

161 – 167

7

Helix

168 – 176

9

Beta strand

183 – 186

4

Helix

196 – 201

6

Helix

202 – 210

9

Beta strand

218 – 222

5

Turn

224 – 226

3

Helix

233 – 242

10

Helix

243 – 245

3

Helix

250 – 259

10

Beta strand

262 – 267

6

Helix

274 – 281

8

Beta strand

287 – 289

3

Helix

293 – 295

3

Turn

296 – 300

5

Sequences

	Sequence		Length	Mass (Da)
	P63458 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: BB7BCD8217FC66C8	FASTA	302	30,788
	10 20 30 40 50 60 MIALLAPGQG SQTEGMLSPW LQLPGAADQI AAWSKAADLD LARLGTTAST EEITDTAVAQ 70 80 90 100 110 120 PLIVAATLLA HQELARRCVL AGKDVIVAGH SVGEIAAYAI AGVIAADDAV ALAATRGAEM 130 140 150 160 170 180 AKACATEPTG MSAVLGGDET EVLSRLEQLD LVPANRNAAG QIVAAGRLTA LEKLAEDPPA 190 200 210 220 230 240 KARVRALGVA GAFHTEFMAP ALDGFAAAAA NIATADPTAT LLSNRDGKPV TSAAAAMDTL 250 260 270 280 290 300 VSQLTQPVRW DLCTATLREH TVTAIVEFPP AGTLSGIAKR ELRGVPARAV KSPADLDELA NL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Identification of substrates of the Mycobacterium tuberculosis proteasome." Pearce M.J., Arora P., Festa R.A., Butler-Wu S.M., Gokhale R.S., Darwin K.H. EMBO J. 25:5423-5432(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEASOME SUBSTRATE. Strain: ATCC 25618 / H37Rv.
[4]	"Ubiquitin-like protein involved in the proteasome pathway of Mycobacterium tuberculosis." Pearce M.J., Mintseris J., Ferreyra J., Gygi S.P., Darwin K.H. Science 322:1104-1107(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-173, MASS SPECTROMETRY, MUTAGENESIS OF LYS-173. Strain: ATCC 25618 / H37Rv.
[5]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
[6]	"Prokayrotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis." Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E., Gygi S.P., Darwin K.H. PLoS ONE 5:E8589-E8589(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-173, IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 25618 / H37Rv.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842579 Genomic DNA. Translation: CAA94639.1.
AE000516 Genomic DNA. Translation: AAK46587.1.
AL123456 Genomic DNA. Translation: CCP45023.1.

PIR

G70778.

RefSeq

NP_216759.1. NC_000962.3.
NP_336773.1. NC_002755.2.
YP_006515666.1. NC_018143.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QC3	X-ray	2.30	A	1-302	[»]
2QJ3	X-ray	3.00	A/B	1-302	[»]

ProteinModelPortal

P63458.

SMR

P63458. Positions 1-302.

ModBase

Search...

Protein-protein interaction databases

STRING

83332.Rv2243.

PTM databases

PhosSite

P0805316.

Proteomic databases

PRIDE

P63458.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK46587; AAK46587; MT2303.

GeneID

13318936.
888769.
924123.

KEGG

mtc:MT2303.
mtu:Rv2243.
mtv:RVBD_2243.

PATRIC

18126813. VBIMycTub22151_2517.

Organism-specific databases

TubercuList

Rv2243.

Phylogenomic databases

eggNOG

COG0331.

HOGENOM

HOG000036505.

KO

K00645.

OMA

AFHTRFM.

ProtClustDB

CLSK872033.

Enzyme and pathway databases

UniPathway

UPA00094.

Family and domain databases

Gene3D

3.40.366.10. 2 hits.

InterPro

IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]

Pfam

PF00698. Acyl_transf_1. 1 hit.
[Graphical view]

SUPFAM

SSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P63458.

Entry information

Entry name

FABD_MYCTU

Accession

Primary (citable) accession number: P63458
Secondary accession number(s): L0TAL7, Q10501

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program