P63457 (FAB2_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 2
EC=2.3.1.41

Alternative name(s):
Beta-ketoacyl-ACP synthase 2
Short name=KAS 2

Gene names

Name:	kasB
Ordered Locus Names:	Mb2270

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the beta-ketoacyl-ACP synthases family.
Sequence caution	The sequence CAD97123.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	3-oxoacyl-[acyl-carrier-protein] synthase 2		PRO_0000180330
Sites
	Active site	170	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63457 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 239F7D19A6380F66	FASTA	417	44,277
	10 20 30 40 50 60 MTELVTGKAF PYVVVTGIAM TTALATDAET TWKLLLDRQS GIRTLDDPFV EEFDLPVRIG 70 80 90 100 110 120 GHLLEEFDHQ LTRIELRRMG YLQRMSTVLS RRLWENAGSP EVDTNRLMVS IGTGLGSAEE 130 140 150 160 170 180 LVFSYDDMRA RGMKAVSPLT VQKYMPNGAA AAVGLERHAK AGVMTPVSAC ASGAEAIARA 190 200 210 220 230 240 WQQIVLGEAD AAICGGVETR IEAVPIAGFA QMRIVMSTNN DDPAGACRPF DRDRDGFVFG 250 260 270 280 290 300 EGGALLLIET EEHAKARGAN ILARIMGASI TSDGFHMVAP DPNGERAGHA ITRAIQLAGL 310 320 330 340 350 360 APGDIDHVNA HATGTQVGDL AEGRAINNAL GGNRPAVYAP KSALGHSVGA VGAVESILTV 370 380 390 400 410 LALRDQVIPP TLNLVNLDPE IDLDVVAGEP RPGNYRYAIN NSFGFGGHNV AIAFGRY

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248341 Genomic DNA. Translation: CAD97123.1. Different initiation.
RefSeq	NP_855919.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P63457.
SMR	P63457. Positions 4-417.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000016251; EBMYCP00000016086; EBMYCG00000016246.
GeneID	1091410.
GenomeReviews	Gene locus Mb2270 in contig BX248333_GR.
KEGG	mbo:Mb2270.
PATRIC	18006692. VBIMycBov88188_2491.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015144.
HOGENOM	HBG757733.
ProtClustDB	PRK07910.
Enzyme and pathway databases
BioCyc	MBOV233413:MB2270-MONOMER.
Family and domain databases
InterPro	IPR000794. Beta-ketoacyl_synthase. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K11609.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00109. ketoacyl-synt. 1 hit. PF02801. Ketoacyl-synt_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00606. B_KETOACYL_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAB2_MYCBO

Accession

Primary (citable) accession number: P63457
Secondary accession number(s): Q10525

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program