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Protein

Probable phospholipid import ATP-binding protein MlaF

Gene

mlaF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane. Probably responsible for energy coupling to the transport system.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 48ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • phospholipid transporter activity Source: EcoCyc

GO - Biological processi

  • phospholipid transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YRBF-MONOMER.
ECOL316407:JW3162-MONOMER.

Protein family/group databases

TCDBi3.A.1.27.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid import ATP-binding protein MlaFCurated (EC:3.6.3.-1 Publication)
Gene namesi
Name:mlaF
Synonyms:yrbF
Ordered Locus Names:b3195, JW3162
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12801. mlaF.

Subcellular locationi

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutation confers sensitivity to SDS-EDTA and leads to accumulation of phospholipid in the outer leaflet of the outer membrane.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000931711 – 269Probable phospholipid import ATP-binding protein MlaFAdd BLAST269

Proteomic databases

EPDiP63386.
PaxDbiP63386.
PRIDEiP63386.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MlaF), two transmembrane proteins (MlaE), two cytoplasmic solute-binding proteins (MlaB) and a periplamic solute-binding protein (MlaD).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F53EBI-561408,EBI-543750

Protein-protein interaction databases

BioGridi4263401. 7 interactors.
DIPiDIP-35865N.
IntActiP63386. 8 interactors.
MINTiMINT-1228070.
STRINGi511145.b3195.

Structurei

3D structure databases

ProteinModelPortaliP63386.
SMRiP63386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 245ABC transporterPROSITE-ProRule annotationAdd BLAST237

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DHJ. Bacteria.
COG1127. LUCA.
InParanoidiP63386.
KOiK02065.
OMAiIRSIVLM.
PhylomeDBiP63386.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQSVANLVD MRDVSFTRGN RCIFDNISLT VPRGKITAIM GPSGIGKTTL
60 70 80 90 100
LRLIGGQIAP DHGEILFDGE NIPAMSRSRL YTVRKRMSML FQSGALFTDM
110 120 130 140 150
NVFDNVAYPL REHTQLPAPL LHSTVMMKLE AVGLRGAAKL MPSELSGGMA
160 170 180 190 200
RRAALARAIA LEPDLIMFDE PFVGQDPITM GVLVKLISEL NSALGVTCVV
210 220 230 240 250
VSHDVPEVLS IADHAWILAD KKIVAHGSAQ ALQANPDPRV RQFLDGIADG
260
PVPFRYPAGD YHADLLPGS
Length:269
Mass (Da):29,097
Last modified:October 11, 2004 - v1
Checksum:i742EF8DEDA742CF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57996.1.
U00096 Genomic DNA. Translation: AAC76227.1.
AP009048 Genomic DNA. Translation: BAE77239.1.
PIRiE65110.
RefSeqiNP_417662.1. NC_000913.3.
WP_000438245.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76227; AAC76227; b3195.
BAE77239; BAE77239; BAE77239.
GeneIDi947729.
KEGGiecj:JW3162.
eco:b3195.
PATRICi32121810. VBIEscCol129921_3289.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57996.1.
U00096 Genomic DNA. Translation: AAC76227.1.
AP009048 Genomic DNA. Translation: BAE77239.1.
PIRiE65110.
RefSeqiNP_417662.1. NC_000913.3.
WP_000438245.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP63386.
SMRiP63386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263401. 7 interactors.
DIPiDIP-35865N.
IntActiP63386. 8 interactors.
MINTiMINT-1228070.
STRINGi511145.b3195.

Protein family/group databases

TCDBi3.A.1.27.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP63386.
PaxDbiP63386.
PRIDEiP63386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76227; AAC76227; b3195.
BAE77239; BAE77239; BAE77239.
GeneIDi947729.
KEGGiecj:JW3162.
eco:b3195.
PATRICi32121810. VBIEscCol129921_3289.

Organism-specific databases

EchoBASEiEB2653.
EcoGeneiEG12801. mlaF.

Phylogenomic databases

eggNOGiENOG4105DHJ. Bacteria.
COG1127. LUCA.
InParanoidiP63386.
KOiK02065.
OMAiIRSIVLM.
PhylomeDBiP63386.

Enzyme and pathway databases

BioCyciEcoCyc:YRBF-MONOMER.
ECOL316407:JW3162-MONOMER.

Miscellaneous databases

PROiP63386.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLAF_ECOLI
AccessioniPrimary (citable) accession number: P63386
Secondary accession number(s): P45393, Q2M917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.