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Protein

Intermembrane phospholipid transport system ATP-binding protein MlaF

Gene

mlaF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MlaFEDB, which is involved in a phospholipid transport pathway that maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane. Responsible for energy coupling to the transport system.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 48ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATP binding Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:YRBF-MONOMER

Protein family/group databases

TCDBi3.A.1.27.3 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Intermembrane phospholipid transport system ATP-binding protein MlaFCurated (EC:3.6.3.-1 Publication)
Gene namesi
Name:mlaF1 Publication
Synonyms:yrbF
Ordered Locus Names:b3195, JW3162
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12801 mlaF

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutation leads to accumulation of phospholipid in the outer leaflet of the outer membrane and increased outer membrane permeability. It confers sensitivity to SDS-EDTA.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000931711 – 269Intermembrane phospholipid transport system ATP-binding protein MlaFAdd BLAST269

Proteomic databases

EPDiP63386
PaxDbiP63386
PRIDEiP63386

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MlaF), two transmembrane proteins (MlaE), two cytoplasmic solute-binding proteins (MlaB) and six periplamic solute-binding proteins (MlaD).1 Publication2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F54EBI-561408,EBI-543750

Protein-protein interaction databases

BioGridi4263401, 121 interactors
DIPiDIP-35865N
IntActiP63386, 8 interactors
STRINGi316385.ECDH10B_3369

Structurei

3D structure databases

ProteinModelPortaliP63386
SMRiP63386
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 245ABC transporterPROSITE-ProRule annotationAdd BLAST237

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DHJ Bacteria
COG1127 LUCA
InParanoidiP63386
KOiK02065
OMAiNLIIGQS
PhylomeDBiP63386

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR030296 MlaF/Mkl
IPR027417 P-loop_NTPase
PANTHERiPTHR43023:SF1 PTHR43023:SF1, 1 hit
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit

Sequencei

Sequence statusi: Complete.

P63386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQSVANLVD MRDVSFTRGN RCIFDNISLT VPRGKITAIM GPSGIGKTTL
60 70 80 90 100
LRLIGGQIAP DHGEILFDGE NIPAMSRSRL YTVRKRMSML FQSGALFTDM
110 120 130 140 150
NVFDNVAYPL REHTQLPAPL LHSTVMMKLE AVGLRGAAKL MPSELSGGMA
160 170 180 190 200
RRAALARAIA LEPDLIMFDE PFVGQDPITM GVLVKLISEL NSALGVTCVV
210 220 230 240 250
VSHDVPEVLS IADHAWILAD KKIVAHGSAQ ALQANPDPRV RQFLDGIADG
260
PVPFRYPAGD YHADLLPGS
Length:269
Mass (Da):29,097
Last modified:October 11, 2004 - v1
Checksum:i742EF8DEDA742CF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57996.1
U00096 Genomic DNA Translation: AAC76227.1
AP009048 Genomic DNA Translation: BAE77239.1
PIRiE65110
RefSeqiNP_417662.1, NC_000913.3
WP_000438245.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76227; AAC76227; b3195
BAE77239; BAE77239; BAE77239
GeneIDi947729
KEGGiecj:JW3162
eco:b3195
PATRICifig|1411691.4.peg.3536

Similar proteinsi

Entry informationi

Entry nameiMLAF_ECOLI
AccessioniPrimary (citable) accession number: P63386
Secondary accession number(s): P45393, Q2M917
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 20, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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