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Protein

Lipid A export ATP-binding/permease protein MsbA

Gene

msbA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi376 – 383ATPUniRule annotation8

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.39. 5542.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A export ATP-binding/permease protein MsbAUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:msbAUniRule annotation
Ordered Locus Names:STM0984
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei64 – 84HelicalUniRule annotationAdd BLAST21
Transmembranei140 – 162HelicalUniRule annotationAdd BLAST23
Transmembranei167 – 186HelicalUniRule annotationAdd BLAST20
Transmembranei253 – 273HelicalUniRule annotationAdd BLAST21
Transmembranei275 – 295HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000925981 – 582Lipid A export ATP-binding/permease protein MsbAAdd BLAST582

Proteomic databases

PaxDbiP63359.
PRIDEiP63359.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-46177N.
STRINGi99287.STM0984.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B5YX-ray4.50A/B/C/D1-582[»]
3B5ZX-ray4.20A/B/C/D1-582[»]
3B60X-ray3.70A/B/C/D1-582[»]
ProteinModelPortaliP63359.
SMRiP63359.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63359.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 310ABC transmembrane type-1UniRule annotationAdd BLAST284
Domaini342 – 578ABC transporterUniRule annotationAdd BLAST237

Domaini

In MsbA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Lipid exporter (TC 3.A.1.106) family. [View classification]UniRule annotation
Contains 1 ABC transmembrane type-1 domain.UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
KOiK11085.
OMAiENCAVVS.
PhylomeDBiP63359.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011917. ABC_transpr_lipidA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR02203. MsbA_lipidA. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51239. MSBA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNDKDLSTW QTFRRLWPTI APFKAGLIVA GIALILNAAS DTFMLSLLKP
60 70 80 90 100
LLDDGFGKTD RSVLLWMPLV VIGLMILRGI TSYISSYCIS WVSGKVVMTM
110 120 130 140 150
RRRLFGHMMG MPVAFFDKQS TGTLLSRITY DSEQVASSSS GALITVVREG
160 170 180 190 200
ASIIGLFIMM FYYSWQLSII LVVLAPIVSI AIRVVSKRFR SISKNMQNTM
210 220 230 240 250
GQVTTSAEQM LKGHKEVLIF GGQEVETKRF DKVSNKMRLQ GMKMVSASSI
260 270 280 290 300
SDPIIQLIAS LALAFVLYAA SFPSVMDSLT AGTITVVFSS MIALMRPLKS
310 320 330 340 350
LTNVNAQFQR GMAACQTLFA ILDSEQEKDE GKRVIDRATG DLEFRNVTFT
360 370 380 390 400
YPGREVPALR NINLKIPAGK TVALVGRSGS GKSTIASLIT RFYDIDEGHI
410 420 430 440 450
LMDGHDLREY TLASLRNQVA LVSQNVHLFN DTVANNIAYA RTEEYSREQI
460 470 480 490 500
EEAARMAYAM DFINKMDNGL DTIIGENGVL LSGGQRQRIA IARALLRDSP
510 520 530 540 550
ILILDEATSA LDTESERAIQ AALDELQKNR TSLVIAHRLS TIEQADEIVV
560 570 580
VEDGIIVERG THSELLAQHG VYAQLHKMQF GQ
Length:582
Mass (Da):64,340
Last modified:October 11, 2004 - v1
Checksum:iA8806F8CC650D538
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL19918.1.
RefSeqiNP_459959.1. NC_003197.1.
WP_000551246.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19918; AAL19918; STM0984.
GeneIDi1252502.
KEGGistm:STM0984.
PATRICi32380381. VBISalEnt20916_1037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL19918.1.
RefSeqiNP_459959.1. NC_003197.1.
WP_000551246.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B5YX-ray4.50A/B/C/D1-582[»]
3B5ZX-ray4.20A/B/C/D1-582[»]
3B60X-ray3.70A/B/C/D1-582[»]
ProteinModelPortaliP63359.
SMRiP63359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46177N.
STRINGi99287.STM0984.

Proteomic databases

PaxDbiP63359.
PRIDEiP63359.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19918; AAL19918; STM0984.
GeneIDi1252502.
KEGGistm:STM0984.
PATRICi32380381. VBISalEnt20916_1037.

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
KOiK11085.
OMAiENCAVVS.
PhylomeDBiP63359.

Enzyme and pathway databases

BRENDAi3.6.3.39. 5542.

Miscellaneous databases

EvolutionaryTraceiP63359.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011917. ABC_transpr_lipidA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR02203. MsbA_lipidA. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51239. MSBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSBA_SALTY
AccessioniPrimary (citable) accession number: P63359
Secondary accession number(s): Q8XFG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.