Reviewed, UniProtKB/Swiss-Prot P63354 (CYSA_BRUSU)

Names and origin

Protein names

Recommended name:
    Sulfate/thiosulfate import ATP-binding protein cysA
    EC=3.6.3.25
Alternative name(s):
    Sulfate-transporting ATPase

Gene names

Name:	cysA
Ordered Locus Names:	BR0110

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the ABC transporter complex cysAWTP involved in sulfate/thiosulfate import. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + sulfate(Out) = ADP + phosphate + sulfate(In). HAMAP MF_01701
Subunit structure	The complex is composed of two ATP-binding proteins (cysA), two transmembrane proteins (cysT and cysW) and a solute-binding protein (cysP) Probable.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Sulfate transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate transport Inferred from electronic annotation. Source: HAMAP
Cellular component	ATP-binding cassette (ABC) transporter complex Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate transmembrane-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Sulfate/thiosulfate import ATP-binding protein cysA HAMAP MF_01701		PRO_0000092258
Regions
	Domain	3 – 237	235	ABC transporter
	Nucleotide binding	35 – 42	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P63354-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 3F23D25526E49473	FASTA	359	39,601
	10 20 30 40 50 60 MEVRVAGVRK EFARFPALHN VSLTIQSGEL IALLGPSGSG KTTLLRLIAG LETPTEGMIF 70 80 90 100 110 120 FGDEDASQKS VQERNVGFVF QHYALFRHMT VADNIGFGLK VRPGGTRPST AEIRRRALEL 130 140 150 160 170 180 LDLVQLSGLE KRYPAQLSGG QRQRVALARA MAIEPRVLLL DEPFGALDAQ VRKELRRWLR 190 200 210 220 230 240 EIHDKTGHTT VFVTHDQDEA LELADRVVVM SQGRIEQVGT PDEVYDKPNS PFVYGFIGES 250 260 270 280 290 300 STLPVRVENG EVWLADRNIG LGAENMPDGD AQLYFRPHDV ELLDGCGGCI AGTVIASRRS 310 320 330 340 350 GGKRRVELEV GGARERIEIE IPAEHPAAEK SRIAFRPRYW TLFRAGDSEL PAPKAAATT

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29066.1.
RefSeq	NP_697151.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1165767.
GenomeReviews	Gene locus BR0110 in contig AE014291_GR.
KEGG	bms:BR0110.
TIGR	BR0110.
Phylogenomic databases
HOGENOM	P63354.
OMA	QRQVGFV
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0110-MON.
BRENDA	3.6.3.25. 281610.
Family and domain databases
HAMAP	MF_01701. [Tree]
InterPro	IPR014769. ABC_CysA_ATP_bd_C. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR003593. ATPase_AAA+_core. IPR008995. Mo/tungstate-bd. IPR005666. Sulph_transpt1. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00968. 3a0106s01. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51237. CYSA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSA_BRUSU

Accession

Primary (citable) accession number: P63354
Secondary accession number(s): Q8G342, Q8YEP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 24, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)