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Protein

Proteasome-associated ATPase

Gene

mpa

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATPase which is responsible for recognizing, binding, unfolding and translocation of pupylated proteins into the bacterial 20S proteasome core particle. May be essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase may function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis.UniRule annotation

Pathwayi: proteasomal Pup-dependent pathway

This protein is involved in the pathway proteasomal Pup-dependent pathway, which is part of Protein degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway proteasomal Pup-dependent pathway and in Protein degradation.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi296 – 301ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-2205-MONOMER
UniPathwayiUPA00997

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-associated ATPaseUniRule annotation
Alternative name(s):
AAA ATPase forming ring-shaped complexesUniRule annotation
Short name:
ARCUniRule annotation
Mycobacterial proteasome ATPaseUniRule annotation
Gene namesi
Name:mpaUniRule annotation
Ordered Locus Names:BQ2027_MB2139C
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847791 – 609Proteasome-associated ATPaseAdd BLAST609

Proteomic databases

PRIDEiP63346

Interactioni

Subunit structurei

Homohexamer. Assembles into a hexameric ring structure that caps the 20S proteasome core. Strongly interacts with the prokaryotic ubiquitin-like protein Pup through a hydrophobic interface; the interacting region of ARC lies in its N-terminal coiled-coil domain. There is one Pup binding site per ARC hexamer ring. Upon ATP-binding, the C-terminus of ARC interacts with the alpha-rings of the proteasome core, possibly by binding to the intersubunit pockets.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP63346
SMRiP63346
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni608 – 609Docks into pockets in the proteasome alpha-ringUniRule annotation2

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili20 – 96UniRule annotationAdd BLAST77

Domaini

Consists of three main regions, an N-terminal coiled-coil domain that binds to protein Pup and functions as a docking station, an interdomain involved in ARC hexamerization, and a C-terminal ATPase domain of the AAA type.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000245286
OMAiCVDEFKE

Family and domain databases

HAMAPiMF_02112 ARC_ATPase, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
IPR032501 Prot_ATP_ID_OB
IPR022482 Proteasome_ATPase
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF16450 Prot_ATP_ID_OB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR03689 pup_AAA, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

P63346-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGESERSEAF GIPRDSPLSS GDAAELEQLR REAAVLREQL ENAVGSHAPT
60 70 80 90 100
RSARDIHQLE ARIDSLAARN SKLMETLKEA RQQLLALREE VDRLGQPPSG
110 120 130 140 150
YGVLLATHDD DTVDVFTSGR KMRLTCSPNI DAASLKKGQT VRLNEALTVV
160 170 180 190 200
EAGTFEAVGE ISTLREILAD GHRALVVGHA DEERVVWLAD PLIAEDLPDG
210 220 230 240 250
LPEALNDDTR PRKLRPGDSL LVDTKAGYAF ERIPKAEVED LVLEEVPDVS
260 270 280 290 300
YADIGGLSRQ IEQIRDAVEL PFLHKELYRE YSLRPPKGVL LYGPPGCGKT
310 320 330 340 350
LIAKAVANSL AKKMAEVRGD DAHEAKSYFL NIKGPELLNK FVGETERHIR
360 370 380 390 400
LIFQRAREKA SEGTPVIVFF DEMDSIFRTR GTGVSSDVET TVVPQLLSEI
410 420 430 440 450
DGVEGLENVI VIGASNREDM IDPAILRPGR LDVKIKIERP DAEAAQDIYS
460 470 480 490 500
KYLTEFLPVH ADDLAEFDGD RSACIKAMIE KVVDRMYAEI DDNRFLEVTY
510 520 530 540 550
ANGDKEVMYF KDFNSGAMIQ NVVDRAKKNA IKSVLETGQP GLRIQHLLDS
560 570 580 590 600
IVDEFAENED LPNTTNPDDW ARISGKKGER IVYIRTLVTG KSSSASRAID

TESNLGQYL
Length:609
Mass (Da):67,401
Last modified:October 11, 2004 - v1
Checksum:i4D5F4E630614C58D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU00746.1
RefSeqiNP_855788.1, NC_002945.3
WP_003411035.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO43392; CDO43392; Mb2139c
PATRICifig|233413.5.peg.2352

Similar proteinsi

Entry informationi

Entry nameiARC_MYCBO
AccessioniPrimary (citable) accession number: P63346
Secondary accession number(s): A0A1R3Y0B3, O33250, X2BJF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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