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P63331

- PP2AA_RAT

UniProt

P63331 - PP2AA_RAT

Protein

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Gene

Ppp2ca

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Activates RAF1 by dephosphorylating it at 'Ser-259' By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Manganese 1By similarity
    Metal bindingi59 – 591Manganese 1By similarity
    Metal bindingi85 – 851Manganese 1By similarity
    Metal bindingi85 – 851Manganese 2By similarity
    Metal bindingi117 – 1171Manganese 2By similarity
    Active sitei118 – 1181Proton donorBy similarity
    Metal bindingi167 – 1671Manganese 2By similarity
    Metal bindingi241 – 2411Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein C-terminus binding Source: RGD
    4. protein dimerization activity Source: MGI
    5. protein heterodimerization activity Source: UniProtKB
    6. protein serine/threonine phosphatase activity Source: RGD

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW
    2. mesoderm development Source: Ensembl
    3. negative regulation of epithelial to mesenchymal transition Source: Ensembl
    4. positive regulation of protein serine/threonine kinase activity Source: Ensembl
    5. protein dephosphorylation Source: RGD
    6. protein heterotrimerization Source: MGI
    7. regulation of protein autophosphorylation Source: MGI
    8. regulation of protein catabolic process Source: MGI
    9. regulation of protein phosphorylation Source: MGI
    10. regulation of receptor activity Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Meiosis

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.
    REACT_198369. Initiation of Nuclear Envelope Reformation.
    REACT_198611. Resolution of Sister Chromatid Cohesion.
    REACT_198842. MASTL Facilitates Mitotic Progression.
    REACT_199007. Spry regulation of FGF signaling.
    REACT_216976. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_225694. Glycolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (EC:3.1.3.16)
    Short name:
    PP2A-alpha
    Gene namesi
    Name:Ppp2ca
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi3380. Ppp2ca.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle pole By similarity
    Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 By similarity.By similarity

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB-SubCell
    2. cytosol Source: Ensembl
    3. nucleus Source: UniProtKB-SubCell
    4. plasma membrane Source: Ensembl
    5. protein phosphatase type 2A complex Source: Ensembl
    6. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoformPRO_0000058843Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei307 – 3071PhosphotyrosineBy similarity
    Modified residuei309 – 3091Leucine methyl esterBy similarity

    Post-translational modificationi

    Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (Lcmt1) and protein phosphatase methylesterase 1 (Ppme1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.By similarity
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation By similarity.By similarity

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    PaxDbiP63331.
    PRIDEiP63331.

    2D gel databases

    World-2DPAGE0004:P63331.

    PTM databases

    PhosphoSiteiP63331.

    Expressioni

    Gene expression databases

    GenevestigatoriP63331.

    Interactioni

    Subunit structurei

    PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct. Interacts with TP53, SGOL1 and SGOL2. Interacts with AXIN1; the interaction dephosphorylates AXIN1 By similarity. Interacts with PIM3; this interaction promotes dephosphorylation, ubiquitination and proteasomal degradation of PIM3 By similarity. Interacts with RAF1. Interacts with GSK3B (via C2 domain) By similarity. Interaction with IGBP1 protects unassembled PPP2CA from degradative ubiquitination By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ANP32AP396872EBI-7050205,EBI-359234From a different organism.
    Dpysl2P479422EBI-7050205,EBI-917570
    TIPRLO756633EBI-7050205,EBI-1054735From a different organism.

    Protein-protein interaction databases

    BioGridi246804. 2 interactions.
    IntActiP63331. 8 interactions.
    MINTiMINT-199689.

    Structurei

    3D structure databases

    ProteinModelPortaliP63331.
    SMRiP63331. Positions 2-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00550000074618.
    HOGENOMiHOG000172696.
    HOVERGENiHBG000216.
    InParanoidiP63331.
    KOiK04382.
    OMAiQVKTLCD.
    OrthoDBiEOG74N5H2.
    PhylomeDBiP63331.
    TreeFamiTF105559.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63331-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDEKLFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC    50
    PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL 100
    VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF 150
    DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW 200
    SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW 250
    CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV 300
    TRRTPDYFL 309
    Length:309
    Mass (Da):35,608
    Last modified:October 11, 2004 - v1
    Checksum:i8DC11276E6DF9E33
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16043 mRNA. Translation: CAA34166.1.
    X14159 mRNA. Translation: CAB42983.1.
    M33114 mRNA. Translation: AAA41904.1.
    BC070914 mRNA. Translation: AAH70914.1.
    BC072531 mRNA. Translation: AAH72531.1.
    PIRiS06592. PART2A.
    RefSeqiNP_058735.1. NM_017039.2.
    UniGeneiRn.1271.

    Genome annotation databases

    EnsembliENSRNOT00000007621; ENSRNOP00000007621; ENSRNOG00000005389.
    GeneIDi24672.
    KEGGirno:24672.
    UCSCiRGD:3380. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16043 mRNA. Translation: CAA34166.1 .
    X14159 mRNA. Translation: CAB42983.1 .
    M33114 mRNA. Translation: AAA41904.1 .
    BC070914 mRNA. Translation: AAH70914.1 .
    BC072531 mRNA. Translation: AAH72531.1 .
    PIRi S06592. PART2A.
    RefSeqi NP_058735.1. NM_017039.2.
    UniGenei Rn.1271.

    3D structure databases

    ProteinModelPortali P63331.
    SMRi P63331. Positions 2-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246804. 2 interactions.
    IntActi P63331. 8 interactions.
    MINTi MINT-199689.

    Chemistry

    BindingDBi P63331.

    PTM databases

    PhosphoSitei P63331.

    2D gel databases

    World-2DPAGE 0004:P63331.

    Proteomic databases

    PaxDbi P63331.
    PRIDEi P63331.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000007621 ; ENSRNOP00000007621 ; ENSRNOG00000005389 .
    GeneIDi 24672.
    KEGGi rno:24672.
    UCSCi RGD:3380. rat.

    Organism-specific databases

    CTDi 5515.
    RGDi 3380. Ppp2ca.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00550000074618.
    HOGENOMi HOG000172696.
    HOVERGENi HBG000216.
    InParanoidi P63331.
    KOi K04382.
    OMAi QVKTLCD.
    OrthoDBi EOG74N5H2.
    PhylomeDBi P63331.
    TreeFami TF105559.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.
    REACT_198369. Initiation of Nuclear Envelope Reformation.
    REACT_198611. Resolution of Sister Chromatid Cohesion.
    REACT_198842. MASTL Facilitates Mitotic Progression.
    REACT_199007. Spry regulation of FGF signaling.
    REACT_216976. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_225694. Glycolysis.

    Miscellaneous databases

    NextBioi 295524.

    Gene expression databases

    Genevestigatori P63331.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a rat heart cDNA encoding the isotype alpha of the catalytic subunit of protein phosphatase 2A."
      Posas F., Arino J.
      Nucleic Acids Res. 17:8369-8369(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. "Molecular cloning of cDNA for the catalytic subunit of rat liver type 2A protein phosphatase, and detection of high levels of expression of the gene in normal and cancer cells."
      Kitagawa Y., Tahira T., Ikeda I., Kikuchi K., Tsuiki S., Sugimura T., Nagao M.
      Biochim. Biophys. Acta 951:123-129(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart and Lung.

    Entry informationi

    Entry nameiPP2AA_RAT
    AccessioniPrimary (citable) accession number: P63331
    Secondary accession number(s): O88591, P13353
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3