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Protein

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Gene

Ppp2ca

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Activates RAF1 by dephosphorylating it at 'Ser-259' (By similarity).By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57Manganese 1By similarity1
Metal bindingi59Manganese 1By similarity1
Metal bindingi85Manganese 1By similarity1
Metal bindingi85Manganese 2By similarity1
Metal bindingi117Manganese 2By similarity1
Active sitei118Proton donorBy similarity1
Metal bindingi167Manganese 2By similarity1
Metal bindingi241Manganese 2By similarity1

GO - Molecular functioni

  • GABA receptor binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • protein C-terminus binding Source: MGI
  • protein dimerization activity Source: MGI
  • protein heterodimerization activity Source: UniProtKB
  • protein serine/threonine phosphatase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1295596. Spry regulation of FGF signaling.
R-MMU-180024. DARPP-32 events.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-196299. Beta-catenin phosphorylation cascade.
R-MMU-198753. ERK/MAPK targets.
R-MMU-202670. ERKs are inactivated.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-2995383. Initiation of Nuclear Envelope Reformation.
R-MMU-389513. CTLA4 inhibitory signaling.
R-MMU-4641262. Disassembly of the destruction complex and recruitment of AXIN to the membrane.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-5673000. RAF activation.
R-MMU-5675221. Negative regulation of MAPK pathway.
R-MMU-6804757. Regulation of TP53 Degradation.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-MMU-68877. Mitotic Prometaphase.
R-MMU-69273. Cyclin A/B1 associated events during G2/M transition.
R-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (EC:3.1.3.16)
Short name:
PP2A-alpha
Gene namesi
Name:Ppp2ca
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1321159. Ppp2ca.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: UniProtKB
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: MGI
  • protein phosphatase type 2A complex Source: MGI
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi307Y → Q: Loss of trimeric subunit ABC assembly. 1 Publication1
Mutagenesisi309L → A: Loss of binding to PP2A B-alpha regulatory subunit. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588401 – 309Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoformAdd BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei307Phosphotyrosine1 Publication1
Modified residuei309Leucine methyl ester1 Publication1

Post-translational modificationi

Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (Lcmt1) and protein phosphatase methylesterase 1 (Ppme1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.1 Publication
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.1 Publication
May be monoubiquitinated by NOSIP.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP63330.
MaxQBiP63330.
PaxDbiP63330.
PeptideAtlasiP63330.
PRIDEiP63330.

PTM databases

iPTMnetiP63330.
PhosphoSitePlusiP63330.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020349.
GenevisibleiP63330. MM.

Interactioni

Subunit structurei

PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct. Interacts with TP53, SGO1 and SGO2. Interacts with AXIN1; the interaction dephosphorylates AXIN1 (By similarity). Interacts with PIM3; this interaction promotes dephosphorylation, ubiquitination and proteasomal degradation of PIM3 (By similarity). Interacts with RAF1. Interacts with GSK3B (via C2 domain) (By similarity). Interaction with IGBP1 protects unassembled PPP2CA from degradative ubiquitination (By similarity). Interacts with KCTD20 (PubMed:24156551). Interacts with BTBD10 (PubMed:18160256).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Igbp1Q612492EBI-397144,EBI-7002233
Ppp2r1aQ76MZ33EBI-397144,EBI-400413

GO - Molecular functioni

  • GABA receptor binding Source: MGI
  • protein C-terminus binding Source: MGI
  • protein dimerization activity Source: MGI
  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi202341. 76 interactors.
IntActiP63330. 65 interactors.
MINTiMINT-4105987.
STRINGi10090.ENSMUSP00000020608.

Structurei

3D structure databases

ProteinModelPortaliP63330.
SMRiP63330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0371. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiP63330.
KOiK04382.
OMAiWIENLMA.
OrthoDBiEOG091G0B6S.
PhylomeDBiP63330.
TreeFamiTF105559.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63330-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEKLFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC
60 70 80 90 100
PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL
110 120 130 140 150
VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF
160 170 180 190 200
DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW
210 220 230 240 250
SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW
260 270 280 290 300
CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV

TRRTPDYFL
Length:309
Mass (Da):35,608
Last modified:October 11, 2004 - v1
Checksum:i8DC11276E6DF9E33
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31L → P in AAD12587 (PubMed:9920888).Curated1
Sequence conflicti73G → V in AAD12587 (PubMed:9920888).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076192 mRNA. Translation: AAD12587.1.
Z67745 mRNA. Translation: CAA91558.1.
AK076110 mRNA. Translation: BAC36190.1.
AK172644 mRNA. Translation: BAE43111.1.
AL935177 Genomic DNA. Translation: CAI25806.1.
BC003856 mRNA. Translation: AAH03856.1.
BC054458 mRNA. Translation: AAH54458.1.
CCDSiCCDS24666.1.
RefSeqiNP_062284.1. NM_019411.4.
UniGeneiMm.260288.

Genome annotation databases

EnsembliENSMUST00000020608; ENSMUSP00000020608; ENSMUSG00000020349.
GeneIDi19052.
KEGGimmu:19052.
UCSCiuc007ivb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076192 mRNA. Translation: AAD12587.1.
Z67745 mRNA. Translation: CAA91558.1.
AK076110 mRNA. Translation: BAC36190.1.
AK172644 mRNA. Translation: BAE43111.1.
AL935177 Genomic DNA. Translation: CAI25806.1.
BC003856 mRNA. Translation: AAH03856.1.
BC054458 mRNA. Translation: AAH54458.1.
CCDSiCCDS24666.1.
RefSeqiNP_062284.1. NM_019411.4.
UniGeneiMm.260288.

3D structure databases

ProteinModelPortaliP63330.
SMRiP63330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202341. 76 interactors.
IntActiP63330. 65 interactors.
MINTiMINT-4105987.
STRINGi10090.ENSMUSP00000020608.

PTM databases

iPTMnetiP63330.
PhosphoSitePlusiP63330.

Proteomic databases

EPDiP63330.
MaxQBiP63330.
PaxDbiP63330.
PeptideAtlasiP63330.
PRIDEiP63330.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020608; ENSMUSP00000020608; ENSMUSG00000020349.
GeneIDi19052.
KEGGimmu:19052.
UCSCiuc007ivb.1. mouse.

Organism-specific databases

CTDi5515.
MGIiMGI:1321159. Ppp2ca.

Phylogenomic databases

eggNOGiKOG0371. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiP63330.
KOiK04382.
OMAiWIENLMA.
OrthoDBiEOG091G0B6S.
PhylomeDBiP63330.
TreeFamiTF105559.

Enzyme and pathway databases

ReactomeiR-MMU-1295596. Spry regulation of FGF signaling.
R-MMU-180024. DARPP-32 events.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-196299. Beta-catenin phosphorylation cascade.
R-MMU-198753. ERK/MAPK targets.
R-MMU-202670. ERKs are inactivated.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-2995383. Initiation of Nuclear Envelope Reformation.
R-MMU-389513. CTLA4 inhibitory signaling.
R-MMU-4641262. Disassembly of the destruction complex and recruitment of AXIN to the membrane.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-5673000. RAF activation.
R-MMU-5675221. Negative regulation of MAPK pathway.
R-MMU-6804757. Regulation of TP53 Degradation.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-MMU-68877. Mitotic Prometaphase.
R-MMU-69273. Cyclin A/B1 associated events during G2/M transition.
R-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

ChiTaRSiPpp2ca. mouse.
PROiP63330.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020349.
GenevisibleiP63330. MM.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP2AA_MOUSE
AccessioniPrimary (citable) accession number: P63330
Secondary accession number(s): O88591, P13353, Q5SNY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Double mutation Phe-307 and Gln-309 results in association of the PP2A C subunit with alpha-4 protein.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.