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P63329

- PP2BA_RAT

UniProt

P63329 - PP2BA_RAT

Protein

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Gene

Ppp3ca

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1 By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 1 Fe3+ ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi90 – 901IronBy similarity
    Metal bindingi92 – 921IronBy similarity
    Metal bindingi118 – 1181IronBy similarity
    Metal bindingi118 – 1181ZincBy similarity
    Metal bindingi150 – 1501ZincBy similarity
    Active sitei151 – 1511Proton donorBy similarity
    Metal bindingi199 – 1991ZincBy similarity
    Metal bindingi281 – 2811ZincBy similarity

    GO - Molecular functioni

    1. calcium-dependent protein serine/threonine phosphatase activity Source: RGD
    2. calmodulin binding Source: RGD
    3. calmodulin-dependent protein phosphatase activity Source: UniProtKB
    4. drug binding Source: Ensembl
    5. metal ion binding Source: UniProtKB-KW
    6. phosphoprotein phosphatase activity Source: RGD
    7. protein binding Source: UniProtKB
    8. protein heterodimerization activity Source: RGD
    9. protein serine/threonine phosphatase activity Source: RGD

    GO - Biological processi

    1. calcineurin-NFAT signaling cascade Source: Ensembl
    2. calcium ion transport Source: Ensembl
    3. cellular response to drug Source: Ensembl
    4. cellular response to glucose stimulus Source: BHF-UCL
    5. G1/S transition of mitotic cell cycle Source: Ensembl
    6. multicellular organismal response to stress Source: Ensembl
    7. negative regulation of calcium ion-dependent exocytosis Source: BHF-UCL
    8. negative regulation of insulin secretion Source: BHF-UCL
    9. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    10. protein dephosphorylation Source: RGD
    11. protein import into nucleus Source: Ensembl
    12. regulation of excitatory postsynaptic membrane potential Source: Ensembl
    13. regulation of synaptic transmission Source: Ensembl
    14. response to amphetamine Source: RGD
    15. response to calcium ion Source: UniProtKB
    16. skeletal muscle fiber development Source: Ensembl
    17. transition between fast and slow fiber Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Calmodulin-binding, Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_194690. Ca2+ pathway.
    REACT_203734. FCERI mediated Ca+2 mobilization.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (EC:3.1.3.16)
    Alternative name(s):
    CAM-PRP catalytic subunit
    Calmodulin-dependent calcineurin A subunit alpha isoform
    Gene namesi
    Name:Ppp3ca
    Synonyms:Calna
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi3382. Ppp3ca.

    Subcellular locationi

    Cell membrane By similarity. Cell membranesarcolemma By similarity. Nucleus By similarity
    Note: Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.By similarity

    GO - Cellular componenti

    1. calcineurin complex Source: RGD
    2. cytosol Source: RGD
    3. membrane Source: RGD
    4. mitochondrion Source: Ensembl
    5. nucleus Source: UniProtKB-SubCell
    6. sarcolemma Source: UniProtKB-SubCell
    7. Z disc Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 521520Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoformPRO_0000058824Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei224 – 2241Nitrated tyrosineBy similarity

    Keywords - PTMi

    Acetylation, Nitration

    Proteomic databases

    PaxDbiP63329.
    PRIDEiP63329.

    PTM databases

    PhosphoSiteiP63329.

    Expressioni

    Gene expression databases

    GenevestigatoriP63329.

    Interactioni

    Subunit structurei

    Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3 Interacts with DNM1L; the interaction dephosphorylates DNM1L and regulates its translocation to mitochondria. Interacts with CIB1; the interaction increases upon cardiomyocytes hypertrophy. Interacts with CMYA5; this interaction represses calcineurin activity in muscle By similarity. Interacts with CHP1 and CHP2.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cacna1cP220025EBI-7022944,EBI-1185084
    Cacng8Q8VHW56EBI-7022944,EBI-9086576

    Protein-protein interaction databases

    BioGridi246806. 3 interactions.
    IntActiP63329. 4 interactions.
    MINTiMINT-4106043.

    Structurei

    Secondary structure

    1
    521
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi31 – 344
    Helixi43 – 519
    Helixi58 – 7215
    Beta strandi77 – 815
    Beta strandi83 – 886
    Helixi95 – 10511
    Turni108 – 1103
    Beta strandi113 – 1153
    Beta strandi120 – 1234
    Helixi126 – 13914
    Turni141 – 1433
    Beta strandi144 – 1463
    Helixi154 – 1596
    Helixi162 – 1687
    Helixi172 – 18413
    Beta strandi188 – 1914
    Turni192 – 1943
    Beta strandi195 – 2006
    Helixi209 – 2135
    Beta strandi218 – 2203
    Beta strandi223 – 2253
    Helixi226 – 2327
    Turni237 – 2404
    Beta strandi247 – 2504
    Turni252 – 2554
    Beta strandi256 – 2605
    Helixi262 – 27110
    Beta strandi275 – 2795
    Beta strandi287 – 2904
    Turni295 – 2973
    Beta strandi299 – 3068
    Helixi311 – 3133
    Beta strandi319 – 3257
    Beta strandi328 – 3347
    Helixi344 – 3463
    Helixi349 – 36618
    Helixi473 – 4764
    Helixi478 – 4814

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IL1X-ray3.00A/B/C/D1-492[»]
    ProteinModelPortaliP63329.
    SMRiP63329. Positions 1-411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 301300CatalyticAdd
    BLAST
    Regioni247 – 2537Calcineurin B binding-site 1Sequence Analysis
    Regioni296 – 3016Calcineurin B binding-site 2Sequence Analysis
    Regioni392 – 41423Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni465 – 48723Inhibitory domainAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000063087.
    HOGENOMiHOG000172699.
    HOVERGENiHBG002819.
    InParanoidiP63329.
    KOiK04348.
    OMAiMFWSPED.
    OrthoDBiEOG7BZVSC.
    PhylomeDBiP63329.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P63329-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSEPKAIDPK LSTTDRVVKA VPFPPSHRLT AKEVFDNDGK PRVDILKAHL    50
    MKEGRLEESV ALRIITEGAS ILRQEKNLLD IDAPVTVCGD IHGQFFDLMK 100
    LFEVGGSPAN TRYLFLGDYV DRGYFSIECV LYLWALKILY PKTLFLLRGN 150
    HECRHLTEYF TFKQECKIKY SERVYDACMD AFDCLPLAAL MNQQFLCVHG 200
    GLSPEINTLD DIRKLDRFKE PPAYGPMCDI LWSDPLEDFG NEKTQEHFTH 250
    NTVRGCSYFY SYPAVCDFLQ HNNLLSILRA HEAQDAGYRM YRKSQTTGFP 300
    SLITIFSAPN YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF 350
    TWSLPFVGEK VTEMLVNVLN ICSDDELGSE EDGFDGATAA ARKEVIRNKI 400
    RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLSGG KQTLQSATVE 450
    AIEADEAIKG FSPQHKITSF EEAKGLDRIN ERMPPRRDAM PSDANLNSIN 500
    KALASETNGT DSNGSNSSNI Q 521
    Length:521
    Mass (Da):58,644
    Last modified:October 11, 2004 - v1
    Checksum:i16530C27DDBF1F05
    GO
    Isoform 2 (identifier: P63329-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         448-457: Missing.

    Show »
    Length:511
    Mass (Da):57,615
    Checksum:i01428D70D67450BD
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei448 – 45710Missing in isoform 2. 1 PublicationVSP_018564

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90035 mRNA. Translation: BAA14083.1.
    M29275 mRNA. Translation: AAA40940.1.
    X57115 mRNA. Translation: CAA40398.2.
    D10480 Genomic DNA. Translation: BAA01283.1.
    M58440 mRNA. Translation: AAA41914.1.
    PIRiA33264.
    RefSeqiNP_058737.1. NM_017041.1. [P63329-1]
    XP_006233425.1. XM_006233363.1. [P63329-2]
    UniGeneiRn.228648.
    Rn.6866.

    Genome annotation databases

    EnsembliENSRNOT00000047975; ENSRNOP00000049674; ENSRNOG00000009882. [P63329-2]
    GeneIDi24674.
    KEGGirno:24674.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90035 mRNA. Translation: BAA14083.1 .
    M29275 mRNA. Translation: AAA40940.1 .
    X57115 mRNA. Translation: CAA40398.2 .
    D10480 Genomic DNA. Translation: BAA01283.1 .
    M58440 mRNA. Translation: AAA41914.1 .
    PIRi A33264.
    RefSeqi NP_058737.1. NM_017041.1. [P63329-1 ]
    XP_006233425.1. XM_006233363.1. [P63329-2 ]
    UniGenei Rn.228648.
    Rn.6866.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IL1 X-ray 3.00 A/B/C/D 1-492 [» ]
    ProteinModelPortali P63329.
    SMRi P63329. Positions 1-411.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246806. 3 interactions.
    IntActi P63329. 4 interactions.
    MINTi MINT-4106043.

    PTM databases

    PhosphoSitei P63329.

    Proteomic databases

    PaxDbi P63329.
    PRIDEi P63329.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000047975 ; ENSRNOP00000049674 ; ENSRNOG00000009882 . [P63329-2 ]
    GeneIDi 24674.
    KEGGi rno:24674.

    Organism-specific databases

    CTDi 5530.
    RGDi 3382. Ppp3ca.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000063087.
    HOGENOMi HOG000172699.
    HOVERGENi HBG002819.
    InParanoidi P63329.
    KOi K04348.
    OMAi MFWSPED.
    OrthoDBi EOG7BZVSC.
    PhylomeDBi P63329.

    Enzyme and pathway databases

    Reactomei REACT_194690. Ca2+ pathway.
    REACT_203734. FCERI mediated Ca+2 mobilization.

    Miscellaneous databases

    NextBioi 295542.

    Gene expression databases

    Genevestigatori P63329.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete primary structure of calcineurin A, a calmodulin binding protein homologous with protein phosphatases 1 and 2A."
      Ito A., Hashimoto T., Hirai M., Takeda T., Shuntoh H., Kuno T., Tanaka C.
      Biochem. Biophys. Res. Commun. 163:1492-1497(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Characterization of the phosphatase activity of a baculovirus-expressed calcineurin A isoform."
      Perrino B.A., Fong Y.L., Brickey D.A., Saitoh Y., Ushio Y., Fukunaga K., Miyamoto E., Soderling T.R.
      J. Biol. Chem. 267:15965-15969(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
    3. "Molecular cloning and characterization of the promoter region of the calcineurin A alpha gene."
      Chang C., Takeda T., Mukai H., Shuntoh H., Kuno T., Tanaka C.
      Biochem. J. 288:801-805(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
      Tissue: Liver.
    4. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 143-148, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    5. "Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells."
      Wadzinski B.E., Heasley L.E., Johnson G.L.
      J. Biol. Chem. 265:21504-21508(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 236-306.
    6. "Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein."
      Lin X., Sikkink R.A., Rusnak F., Barber D.L.
      J. Biol. Chem. 274:36125-36131(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHP1.
    7. "CHP2 activates the calcineurin/nuclear factor of activated T cells signaling pathway and enhances the oncogenic potential of HEK293 cells."
      Li G.D., Zhang X., Li R., Wang Y.D., Wang Y.L., Han K.J., Qian X.P., Yang C.G., Liu P., Wei Q., Chen W.F., Zhang J., Zhang Y.
      J. Biol. Chem. 283:32660-32668(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHP2.

    Entry informationi

    Entry nameiPP2BA_RAT
    AccessioniPrimary (citable) accession number: P63329
    Secondary accession number(s): P12816
    , P20652, Q6LDJ8, Q9WUV7, Q9Z1I5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3