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Reviewed, UniProtKB/Swiss-Prot P63329 (PP2BA_RAT)

Last modified October 13, 2009. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
    EC=3.1.3.16
Alternative name(s):
    Calmodulin-dependent calcineurin A subunit alpha isoform
    CAM-PRP catalytic subunit
Gene names
Name: Ppp3ca
Synonyms: Calna
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates HSPB1. May also dephosphorylate and activate SSH1. Ref.2

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 Fe3+ ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with TORC2/CRTC2, MYOZ1, MYOZ2 and MYOZ3 By similarity.

Subcellular location

Nucleus By similarity. Note: Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P63329-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P63329-2)

The sequence of this isoform differs from the canonical sequence as follows:
     448-457: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 521521Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
PRO_0000058824

Regions

Region1 – 301301Catalytic
Region247 – 2537Calcineurin B binding-site 1 Potential
Region296 – 3016Calcineurin B binding-site 2 Potential
Region392 – 41423Calmodulin-binding Potential
Region465 – 48723Inhibitory domain

Sites

Active site1511Proton donor By similarity
Metal binding901Iron By similarity
Metal binding921Iron By similarity
Metal binding1181Iron By similarity
Metal binding1181Zinc By similarity
Metal binding1501Zinc By similarity
Metal binding1991Zinc By similarity
Metal binding2811Zinc By similarity

Amino acid modifications

Modified residue4691Phosphoserine By similarity

Natural variations

Alternative sequence448 – 45710Missing in isoform 2.
VSP_018564

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 16530C27DDBF1F05

FASTA52158,644
        10         20         30         40         50         60 
MSEPKAIDPK LSTTDRVVKA VPFPPSHRLT AKEVFDNDGK PRVDILKAHL MKEGRLEESV 

        70         80         90        100        110        120 
ALRIITEGAS ILRQEKNLLD IDAPVTVCGD IHGQFFDLMK LFEVGGSPAN TRYLFLGDYV 

       130        140        150        160        170        180 
DRGYFSIECV LYLWALKILY PKTLFLLRGN HECRHLTEYF TFKQECKIKY SERVYDACMD 

       190        200        210        220        230        240 
AFDCLPLAAL MNQQFLCVHG GLSPEINTLD DIRKLDRFKE PPAYGPMCDI LWSDPLEDFG 

       250        260        270        280        290        300 
NEKTQEHFTH NTVRGCSYFY SYPAVCDFLQ HNNLLSILRA HEAQDAGYRM YRKSQTTGFP 

       310        320        330        340        350        360 
SLITIFSAPN YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF TWSLPFVGEK 

       370        380        390        400        410        420 
VTEMLVNVLN ICSDDELGSE EDGFDGATAA ARKEVIRNKI RAIGKMARVF SVLREESESV 

       430        440        450        460        470        480 
LTLKGLTPTG MLPSGVLSGG KQTLQSATVE AIEADEAIKG FSPQHKITSF EEAKGLDRIN 

       490        500        510        520 
ERMPPRRDAM PSDANLNSIN KALASETNGT DSNGSNSSNI Q 

« Hide

Isoform 2.

Checksum: 01428D70D67450BD
Show »

FASTA51157,615

References

[1]"The complete primary structure of calcineurin A, a calmodulin binding protein homologous with protein phosphatases 1 and 2A."
Ito A., Hashimoto T., Hirai M., Takeda T., Shuntoh H., Kuno T., Tanaka C.
Biochem. Biophys. Res. Commun. 163:1492-1497(1989) [PubMed: 2551293] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Characterization of the phosphatase activity of a baculovirus-expressed calcineurin A isoform."
Perrino B.A., Fong Y.L., Brickey D.A., Saitoh Y., Ushio Y., Fukunaga K., Miyamoto E., Soderling T.R.
J. Biol. Chem. 267:15965-15969(1992) [PubMed: 1322410] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
[3]"Molecular cloning and characterization of the promoter region of the calcineurin A alpha gene."
Chang C., Takeda T., Mukai H., Shuntoh H., Kuno T., Tanaka C.
Biochem. J. 288:801-805(1992) [PubMed: 1335233] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
Tissue: Liver.
[4]Lubec G., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 143-148, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[5]"Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells."
Wadzinski B.E., Heasley L.E., Johnson G.L.
J. Biol. Chem. 265:21504-21508(1990) [PubMed: 2174876] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 236-306.
+Additional computationally mapped references.

Cross-references

Sequence databases

D90035 mRNA. Translation: BAA14083.1.
M29275 mRNA. Translation: AAA40940.1.
X57115 mRNA. Translation: CAA40398.2.
D10480 Genomic DNA. Translation: BAA01283.1.
M58440 mRNA. Translation: AAA41914.1.
IPIIPI00201410.
IPI00559849.
PIRA33264.
RefSeqNP_058737.1.
UniGeneRn.6866

3D structure databases

HSSPHSSP built from PDB template 1M63 based on UniProtKB Q08209.
SMRP63329. Positions 14-385.
ModBaseSearch...

Protein-protein interaction databases

STRINGP63329.

PTM databases

PhosphoSiteP63329.

Proteomic databases

PRIDEP63329.

Genome annotation databases

EnsemblENSRNOT00000013305; ENSRNOP00000013305; ENSRNOG00000009882; Rattus norvegicus. [Genome view]
ENSRNOT00000047975; ENSRNOP00000049674; ENSRNOG00000009882; Rattus norvegicus. [Genome view]
GeneID24674.
KEGGrno:24674.
UCSCNM_017041. rat.
X57115. rat.

Organism-specific databases

CTD24674.
RGD3382. Ppp3ca.

Phylogenomic databases

HOVERGENP63329.

Enzyme and pathway databases

BRENDA3.1.3.16. 248.

Gene expression databases

GenevestigatorP63329.
GermOnlineENSRNOG00000009882. Rattus norvegicus.

Family and domain databases

InterProIPR004843. M-pesterase.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PANTHERPTHR11668. T_phtase_apaH. 1 hit.
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
ProDomPD000252. T_phtase_apaH. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio604055.

Entry information

Entry namePP2BA_RAT
AccessionPrimary (citable) accession number: P63329
Secondary accession number(s): P12816 expand/collapse secondary AC list , P20652, Q6LDJ8, Q9WUV7, Q9Z1I5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: October 13, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents