Reviewed,
UniProtKB/Swiss-Prot P63329 (PP2BA_RAT)
Last modified
October 13, 2009.
Version 48.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform EC=3.1.3.16 Alternative name(s): Calmodulin-dependent calcineurin A subunit alpha isoform CAM-PRP catalytic subunit | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 521 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates HSPB1. May also dephosphorylate and activate SSH1. Ref.2 |
| Catalytic activity | A phosphoprotein + H2O = a protein + phosphate. |
| Cofactor | Binds 1 Fe3+ ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity. |
| Subunit structure | Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with TORC2/CRTC2, MYOZ1, MYOZ2 and MYOZ3 By similarity. |
| Subcellular location | Nucleus By similarity. Note: Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle By similarity. |
| Sequence similarities | Belongs to the PPP phosphatase family. PP-2B subfamily. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P63329-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P63329-2) The sequence of this isoform differs from the canonical sequence as follows: 448-457: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 521 | 521 | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform | PRO_0000058824 | |||||
Regions | |||||||||
| Region | 1 – 301 | 301 | Catalytic | ||||||
| Region | 247 – 253 | 7 | Calcineurin B binding-site 1 Potential | ||||||
| Region | 296 – 301 | 6 | Calcineurin B binding-site 2 Potential | ||||||
| Region | 392 – 414 | 23 | Calmodulin-binding Potential | ||||||
| Region | 465 – 487 | 23 | Inhibitory domain | ||||||
Sites | |||||||||
| Active site | 151 | 1 | Proton donor By similarity | ||||||
| Metal binding | 90 | 1 | Iron By similarity | ||||||
| Metal binding | 92 | 1 | Iron By similarity | ||||||
| Metal binding | 118 | 1 | Iron By similarity | ||||||
| Metal binding | 118 | 1 | Zinc By similarity | ||||||
| Metal binding | 150 | 1 | Zinc By similarity | ||||||
| Metal binding | 199 | 1 | Zinc By similarity | ||||||
| Metal binding | 281 | 1 | Zinc By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 469 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 448 – 457 | 10 | Missing in isoform 2. | VSP_018564 | |||||
Sequences
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References
| [1] | "The complete primary structure of calcineurin A, a calmodulin binding protein homologous with protein phosphatases 1 and 2A." Ito A., Hashimoto T., Hirai M., Takeda T., Shuntoh H., Kuno T., Tanaka C. Biochem. Biophys. Res. Commun. 163:1492-1497(1989) [PubMed: 2551293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "Characterization of the phosphatase activity of a baculovirus-expressed calcineurin A isoform." Perrino B.A., Fong Y.L., Brickey D.A., Saitoh Y., Ushio Y., Fukunaga K., Miyamoto E., Soderling T.R. J. Biol. Chem. 267:15965-15969(1992) [PubMed: 1322410] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION. |
| [3] | "Molecular cloning and characterization of the promoter region of the calcineurin A alpha gene." Chang C., Takeda T., Mukai H., Shuntoh H., Kuno T., Tanaka C. Biochem. J. 288:801-805(1992) [PubMed: 1335233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19. Tissue: Liver. |
| [4] | Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 143-148, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain. |
| [5] | "Multiplicity of protein serine-threonine phosphatases in PC12 pheochromocytoma and FTO-2B hepatoma cells." Wadzinski B.E., Heasley L.E., Johnson G.L. J. Biol. Chem. 265:21504-21508(1990) [PubMed: 2174876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 236-306. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D90035 mRNA. Translation: BAA14083.1. M29275 mRNA. Translation: AAA40940.1. X57115 mRNA. Translation: CAA40398.2. D10480 Genomic DNA. Translation: BAA01283.1. M58440 mRNA. Translation: AAA41914.1. | |
| IPI | IPI00201410. IPI00559849. |
| PIR | A33264. |
| RefSeq | NP_058737.1. |
| UniGene | Rn.6866 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1M63 based on UniProtKB Q08209. |
| SMR | P63329. Positions 14-385. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P63329. |
PTM databases | |
| PhosphoSite | P63329. |
Proteomic databases | |
| PRIDE | P63329. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000013305; ENSRNOP00000013305; ENSRNOG00000009882; Rattus norvegicus. [Genome view] ENSRNOT00000047975; ENSRNOP00000049674; ENSRNOG00000009882; Rattus norvegicus. [Genome view] |
| GeneID | 24674. |
| KEGG | rno:24674. |
| UCSC | NM_017041. rat. X57115. rat. |
Organism-specific databases | |
| CTD | 24674. |
| RGD | 3382. Ppp3ca. |
Phylogenomic databases | |
| HOVERGEN | P63329. |
Enzyme and pathway databases | |
| BRENDA | 3.1.3.16. 248. |
Gene expression databases | |
| Genevestigator | P63329. |
| GermOnline | ENSRNOG00000009882. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR004843. M-pesterase. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view] |
| PANTHER | PTHR11668. T_phtase_apaH. 1 hit. |
| Pfam | PF00149. Metallophos. 1 hit. [Graphical view] |
| PRINTS | PR00114. STPHPHTASE. |
| ProDom | PD000252. T_phtase_apaH. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00156. PP2Ac. 1 hit. [Graphical view] |
| PROSITE | PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 604055. |
Entry information
| Entry name | PP2BA_RAT | ||||||||
| Accession | Primary (citable) accession number: P63329 Secondary accession number(s): P12816  Q9Z1I5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
