ID   RALA_RAT                Reviewed;         206 AA.
AC   P63322; P05810;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   24-NOV-2009, entry version 54.
DE   RecName: Full=Ras-related protein Ral-A;
DE   Flags: Precursor;
GN   Name=Rala; Synonyms=Ral, Ral-a;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   MEDLINE=93326173; PubMed=7687439; DOI=10.1006/bbrc.1993.1855;
RA   Wildey G.M., Viggeswarapu M., Rim S., Denker J.K.;
RT   "Isolation of cDNA clones and tissue expression of rat ral A and ral B
RT   GTP-binding proteins.";
RL   Biochem. Biophys. Res. Commun. 194:552-559(1993).
RN   [2]
RP   INTERACTION WITH RALBP1 AND CDC42, AND MUTAGENESIS OF ASP-49.
RX   MEDLINE=95349625; PubMed=7623849;
RA   Cantor S.B., Urano T., Feig L.A.;
RT   "Identification and characterization of Ral-binding protein 1, a
RT   potential downstream target of Ral GTPases.";
RL   Mol. Cell. Biol. 15:4578-4584(1995).
CC   -!- SUBUNIT: Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have
CC       overlapping binding sites and compete for RALA binding (By
CC       similarity). Interacts with RALGPS1 (By similarity). Interacts
CC       with RALBP1 via its effector domain and with CDC42.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic
CC       side (Potential).
CC   -!- TISSUE SPECIFICITY: Higher levels where found in testes followed
CC       by brain, adrenal gland, pituitary gland, ovary, liver and kidney.
CC       Low expression was found in muscle.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
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DR   EMBL; L19698; AAA42003.1; -; mRNA.
DR   IPI; IPI00203575; -.
DR   PIR; JN0622; JN0622.
DR   RefSeq; NP_112355.1; -.
DR   UniGene; Rn.100380; -.
DR   SMR; P63322; 11-183.
DR   STRING; P63322; -.
DR   PRIDE; P63322; -.
DR   Ensembl; ENSRNOT00000018190; ENSRNOP00000018190; ENSRNOG00000013454; Rattus norvegicus.
DR   GeneID; 81757; -.
DR   KEGG; rno:81757; -.
DR   UCSC; NM_031093; rat.
DR   CTD; 81757; -.
DR   RGD; 619851; Rala.
DR   HOVERGEN; P63322; -.
DR   OMA; SAKRREN; -.
DR   NextBio; 615530; -.
DR   ArrayExpress; P63322; -.
DR   Genevestigator; P63322; -.
DR   GermOnline; ENSRNOG00000013454; Rattus norvegicus.
DR   GO; GO:0005622; C:intracellular; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005525; F:GTP binding; NAS:RGD.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   InterPro; IPR003577; GTPase_Ras.
DR   InterPro; IPR013753; Ras.
DR   InterPro; IPR001806; Ras_GTPase.
DR   InterPro; IPR015591; Ras_Ral_related.
DR   InterPro; IPR020849; Ras_small_GTPase.
DR   InterPro; IPR005225; Small_GTP_bd.
DR   PANTHER; PTHR11708:SF124; Ras_Ral_related; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PRINTS; PR00449; RASTRNSFRMNG.
DR   SMART; SM00173; RAS; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51421; RAS; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; GTP-binding; Lipoprotein; Membrane; Methylation;
KW   Nucleotide-binding; Prenylation.
FT   CHAIN         1    203       Ras-related protein Ral-A.
FT                                /FTId=PRO_0000082695.
FT   PROPEP      204    206       Removed in mature form (By similarity).
FT                                /FTId=PRO_0000281346.
FT   NP_BIND      21     28       GTP (By similarity).
FT   NP_BIND      68     72       GTP (By similarity).
FT   NP_BIND     127    130       GTP (By similarity).
FT   MOTIF        43     51       Effector region (By similarity).
FT   MOD_RES     203    203       Cysteine methyl ester (By similarity).
FT   LIPID       203    203       S-geranylgeranyl cysteine (By
FT                                similarity).
FT   MUTAGEN      49     49       D->N: Loss of RALBP1 binding.
SQ   SEQUENCE   206 AA;  23553 MW;  6974341FA08C1874 CRC64;
     MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS YRKKVVLDGE
     EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES FAATADFREQ ILRVKEDENV
     PFLLVGNKSD LEDKRQVSVE EAKNRADQWN VNYVETSAKT RANVDKVFFD LMREIRARKM
     EDSKEKNGKK KRKSLAKRIR ERCCIL
//