Reviewed, UniProtKB/Swiss-Prot P63322 (RALA_RAT)

Names and origin

Protein names

Recommended name:
    Ras-related protein Ral-A

Gene names

Name:	Rala
Synonyms:	Ral, Ral-a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding By similarity. Interacts with RALGPS1 By similarity. Interacts with RALBP1 via its effector domain and with CDC42. Ref.2
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Tissue specificity	Higher levels where found in testes followed by brain, adrenal gland, pituitary gland, ovary, liver and kidney. Low expression was found in muscle. Ref.1
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Ref.1 Non-traceable author statement. Source: RGD GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Ras-related protein Ral-A		PRO_0000082695
	Propeptide	204 – 206	3	Removed in mature form By similarity		PRO_0000281346
Regions
	Nucleotide binding	21 – 28	8	GTP By similarity
	Nucleotide binding	68 – 72	5	GTP By similarity
	Nucleotide binding	127 – 130	4	GTP By similarity
	Motif	43 – 51	9	Effector region By similarity
Amino acid modifications
	Modified residue	203	1	Cysteine methyl ester By similarity
	Lipidation	203	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	49	1	D → N: Loss of RALBP1 binding. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P63322-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6974341FA08C1874	FASTA	206	23,553
	10 20 30 40 50 60 MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS YRKKVVLDGE 70 80 90 100 110 120 EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES FAATADFREQ ILRVKEDENV 130 140 150 160 170 180 PFLLVGNKSD LEDKRQVSVE EAKNRADQWN VNYVETSAKT RANVDKVFFD LMREIRARKM 190 200 EDSKEKNGKK KRKSLAKRIR ERCCIL

References

[1]	"Isolation of cDNA clones and tissue expression of rat ral A and ral B GTP-binding proteins." Wildey G.M., Viggeswarapu M., Rim S., Denker J.K. Biochem. Biophys. Res. Commun. 194:552-559(1993) [PubMed: 7687439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Identification and characterization of Ral-binding protein 1, a potential downstream target of Ral GTPases." Cantor S.B., Urano T., Feig L.A. Mol. Cell. Biol. 15:4578-4584(1995) [PubMed: 7623849] [Abstract] Cited for: INTERACTION WITH RALBP1 AND CDC42, MUTAGENESIS OF ASP-49.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19698 mRNA. Translation: AAA42003.1.
IPI	IPI00203575.
PIR	JN0622.
RefSeq	NP_112355.1.
UniGene	Rn.100380
3D structure databases
SMR	P63322. Positions 11-178.
ModBase	Search...
Protein-protein interaction databases
STRING	P63322.
Proteomic databases
PRIDE	P63322.
Genome annotation databases
Ensembl	ENSRNOT00000018190; ENSRNOP00000018190; ENSRNOG00000013454; Rattus norvegicus. [Genome view]
GeneID	81757.
KEGG	rno:81757.
UCSC	NM_031093. rat.
Organism-specific databases
CTD	81757.
RGD	619851. Rala.
Phylogenomic databases
eggNOG	roNOG09209.
HOVERGEN	P63322.
InParanoid	P63322.
OMA	SAKRREN.
PhylomeDB	P63322.
Gene expression databases
ArrayExpress	P63322.
Genevestigator	P63322.
GermOnline	ENSRNOG00000013454. Rattus norvegicus.
Family and domain databases
InterPro	IPR003577. GTPase_Ras. IPR013753. Ras. IPR001806. Ras_GTPase. IPR015591. Ras_Ral_related. IPR020849. Ras_small_GTPase. IPR005225. Small_GTP_bd. [Graphical view]
PANTHER	PTHR11708:SF124. Ras_Ral_related. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615530.

Entry information

Entry name

RALA_RAT

Accession

Primary (citable) accession number: P63322
Secondary accession number(s): P05810

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)