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Protein

Ras-related protein Ral-A

Gene

Rala

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity).By similarity

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 288GTPBy similarity
Nucleotide bindingi68 – 725GTPBy similarity
Nucleotide bindingi127 – 1304GTPBy similarity

GO - Molecular functioni

  • Edg-2 lysophosphatidic acid receptor binding Source: UniProtKB
  • GDP binding Source: Ensembl
  • GTPase activity Source: InterPro
  • GTP binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Exocytosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Ral-A
Gene namesi
Name:Rala
Synonyms:Ral, Ral-a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi619851. Rala.

Subcellular locationi

  • Cell surface By similarity
  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Cleavage furrow
  • Midbody By similarity

  • Note: Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA co-localizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491D → N: Loss of RALBP1 binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 203203Ras-related protein Ral-APRO_0000082695Add
BLAST
Propeptidei204 – 2063Removed in mature formBy similarityPRO_0000281346

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei203 – 2031Cysteine methyl esterBy similarity
Lipidationi203 – 2031S-geranylgeranyl cysteineBy similarity

Post-translational modificationi

Prenylation is essential for membrane localization.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP63322.
PRIDEiP63322.

PTM databases

iPTMnetiP63322.
PhosphoSiteiP63322.
SwissPalmiP63322.

Expressioni

Tissue specificityi

Higher levels where found in testes followed by brain, adrenal gland, pituitary gland, ovary, liver and kidney. Low expression was found in muscle.1 Publication

Gene expression databases

GenevisibleiP63322. RN.

Interactioni

Subunit structurei

Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding (By similarity). Interacts with RALGPS1 (By similarity). Interacts with RALBP1 via its effector domain and with CDC42. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249629. 5 interactions.
IntActiP63322. 1 interaction.
MINTiMINT-1866118.
STRINGi10116.ENSRNOP00000018190.

Structurei

3D structure databases

ProteinModelPortaliP63322.
SMRiP63322. Positions 11-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 519Effector region

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP63322.
KOiK07834.
OMAiFAATTEF.
OrthoDBiEOG7QVM41.
PhylomeDBiP63322.
TreeFamiTF312796.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR028412. Ral.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF174. PTHR24070:SF174. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS
60 70 80 90 100
YRKKVVLDGE EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES
110 120 130 140 150
FAATADFREQ ILRVKEDENV PFLLVGNKSD LEDKRQVSVE EAKNRADQWN
160 170 180 190 200
VNYVETSAKT RANVDKVFFD LMREIRARKM EDSKEKNGKK KRKSLAKRIR

ERCCIL
Length:206
Mass (Da):23,553
Last modified:October 11, 2004 - v1
Checksum:i6974341FA08C1874
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19698 mRNA. Translation: AAA42003.1.
PIRiJN0622.
RefSeqiNP_112355.1. NM_031093.2.
XP_006254055.1. XM_006253993.2.
UniGeneiRn.100380.

Genome annotation databases

EnsembliENSRNOT00000018190; ENSRNOP00000018190; ENSRNOG00000013454.
GeneIDi81757.
KEGGirno:81757.
UCSCiRGD:619851. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19698 mRNA. Translation: AAA42003.1.
PIRiJN0622.
RefSeqiNP_112355.1. NM_031093.2.
XP_006254055.1. XM_006253993.2.
UniGeneiRn.100380.

3D structure databases

ProteinModelPortaliP63322.
SMRiP63322. Positions 11-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249629. 5 interactions.
IntActiP63322. 1 interaction.
MINTiMINT-1866118.
STRINGi10116.ENSRNOP00000018190.

PTM databases

iPTMnetiP63322.
PhosphoSiteiP63322.
SwissPalmiP63322.

Proteomic databases

PaxDbiP63322.
PRIDEiP63322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018190; ENSRNOP00000018190; ENSRNOG00000013454.
GeneIDi81757.
KEGGirno:81757.
UCSCiRGD:619851. rat.

Organism-specific databases

CTDi5898.
RGDi619851. Rala.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP63322.
KOiK07834.
OMAiFAATTEF.
OrthoDBiEOG7QVM41.
PhylomeDBiP63322.
TreeFamiTF312796.

Enzyme and pathway databases

ReactomeiR-RNO-1445148. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

NextBioi311812.
PROiP63322.

Gene expression databases

GenevisibleiP63322. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR028412. Ral.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF174. PTHR24070:SF174. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of cDNA clones and tissue expression of rat ral A and ral B GTP-binding proteins."
    Wildey G.M., Viggeswarapu M., Rim S., Denker J.K.
    Biochem. Biophys. Res. Commun. 194:552-559(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Identification and characterization of Ral-binding protein 1, a potential downstream target of Ral GTPases."
    Cantor S.B., Urano T., Feig L.A.
    Mol. Cell. Biol. 15:4578-4584(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RALBP1 AND CDC42, MUTAGENESIS OF ASP-49.

Entry informationi

Entry nameiRALA_RAT
AccessioniPrimary (citable) accession number: P63322
Secondary accession number(s): P05810
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.