Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P63322 (RALA_RAT)

Last modified November 3, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Ras-related protein Ral-A
Gene names
Name: Rala
Synonyms: Ral, Ral-a
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Hide | Top

Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Subunit structure

Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding By similarity. Interacts with RALGPS1 By similarity. Interacts with RALBP1 via its effector domain and with CDC42.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Tissue specificity

Higher levels where found in testes followed by brain, adrenal gland, pituitary gland, ovary, liver and kidney. Low expression was found in muscle. Ref.1

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 203203Ras-related protein Ral-A
PRO_0000082695
Propeptide204 – 2063Removed in mature form By similarity
PRO_0000281346

Regions

Nucleotide binding21 – 288GTP By similarity
Nucleotide binding68 – 725GTP By similarity
Nucleotide binding127 – 1304GTP By similarity
Motif43 – 519Effector region By similarity

Amino acid modifications

Modified residue2031Cysteine methyl ester By similarity
Lipidation2031S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis491D → N: Loss of RALBP1 binding. Ref.2

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P63322-1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 6974341FA08C1874

FASTA20623,553
        10         20         30         40         50         60 
MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS YRKKVVLDGE 

        70         80         90        100        110        120 
EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES FAATADFREQ ILRVKEDENV 

       130        140        150        160        170        180 
PFLLVGNKSD LEDKRQVSVE EAKNRADQWN VNYVETSAKT RANVDKVFFD LMREIRARKM 

       190        200 
EDSKEKNGKK KRKSLAKRIR ERCCIL

« Hide

Hide | Top

References

[1]"Isolation of cDNA clones and tissue expression of rat ral A and ral B GTP-binding proteins."
Wildey G.M., Viggeswarapu M., Rim S., Denker J.K.
Biochem. Biophys. Res. Commun. 194:552-559(1993) [PubMed: 7687439] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"Identification and characterization of Ral-binding protein 1, a potential downstream target of Ral GTPases."
Cantor S.B., Urano T., Feig L.A.
Mol. Cell. Biol. 15:4578-4584(1995) [PubMed: 7623849] [Abstract]
Cited for: INTERACTION WITH RALBP1 AND CDC42, MUTAGENESIS OF ASP-49.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

L19698 mRNA. Translation: AAA42003.1.
IPIIPI00203575.
PIRJN0622.
RefSeqNP_112355.1.
UniGeneRn.100380

3D structure databases

HSSPHSSP built from PDB template 1UAD based on UniProtKB P11233.
SMRP63322. Positions 11-183.
ModBaseSearch...

Protein-protein interaction databases

STRINGP63322.

Proteomic databases

PRIDEP63322.

Genome annotation databases

EnsemblENSRNOT00000018190; ENSRNOP00000018190; ENSRNOG00000013454; Rattus norvegicus. [Genome view]
GeneID81757.
KEGGrno:81757.
UCSCNM_031093. rat.

Organism-specific databases

CTD81757.
RGD619851. Rala.

Phylogenomic databases

HOVERGENP63322.
OMAAKPKGQN.

Gene expression databases

ArrayExpressP63322.
GenevestigatorP63322.
GermOnlineENSRNOG00000013454. Rattus norvegicus.

Family and domain databases

InterProIPR003577. GTPase_Ras.
IPR013753. Ras.
IPR001806. Ras_GTPase.
IPR015591. Ras_Ral_related.
IPR005225. Small_GTP_bd.
[Graphical view]
PANTHERPTHR11708:SF124. Ras_Ral_related. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00173. RAS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio615530.

Hide | Top

Entry information

Entry nameRALA_RAT
AccessionPrimary (citable) accession number: P63322
Secondary accession number(s): P05810
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents