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Protein

Ras-related protein Ral-A

Gene

Rala

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity). The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (By similarity).By similarity1 Publication

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 28GTPBy similarity8
Nucleotide bindingi68 – 72GTPBy similarity5
Nucleotide bindingi127 – 130GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton reorganization Source: MGI
  • cytokinesis Source: UniProtKB
  • exocytosis Source: UniProtKB-KW
  • membrane organization Source: Reactome
  • membrane raft localization Source: UniProtKB
  • neural tube closure Source: CACAO
  • positive regulation of filopodium assembly Source: MGI
  • Ras protein signal transduction Source: InterPro
  • regulation of exocytosis Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division, Exocytosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Ral-A
Gene namesi
Name:Rala
Synonyms:Ral, Ral-a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1927243. Rala.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side
  • Cleavage furrow By similarity
  • MidbodyMidbody ring By similarity

  • Note: Predominantly at the cell surface in the absence of LPA. In the presence of LPA, colocalizes with LPAR1 and LPAR2 in endocytic vesicles.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000826941 – 203Ras-related protein Ral-AAdd BLAST203
PropeptideiPRO_0000281345204 – 206Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203Cysteine methyl esterBy similarity1
Lipidationi203S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Prenylation is essential for membrane localization.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP63321.
MaxQBiP63321.
PaxDbiP63321.
PeptideAtlasiP63321.
PRIDEiP63321.

PTM databases

iPTMnetiP63321.
PhosphoSitePlusiP63321.
SwissPalmiP63321.

Expressioni

Gene expression databases

BgeeiENSMUSG00000008859.
GenevisibleiP63321. MM.

Interactioni

Subunit structurei

Interacts (via effector domain) with RALBP1 (By similarity). Interacts with EXOC2/Sec5 and EXOC8/Exo84; binding to EXOC2 and EXOC8 is mutually exclusive (By similarity). Interacts with Clostridium exoenzyme C3 (By similarity). Interacts with RALGPS1 (By similarity). Interacts with LPAR1 and LPAR2. Interacts with GRK2 in response to LPAR1 activation. RALA and GRK2 binding to LPAR1 is mutually exclusive (By similarity). Interacts with CDC42 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207790. 1 interactor.
IntActiP63321. 5 interactors.
MINTiMINT-1866102.
STRINGi10090.ENSMUSP00000009003.

Structurei

3D structure databases

ProteinModelPortaliP63321.
SMRiP63321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 51Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133672.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP63321.
KOiK07834.
OMAiQSRAQQW.
OrthoDBiEOG091G0UAU.
PhylomeDBiP63321.
TreeFamiTF312796.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR028412. Ral.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
PANTHERiPTHR24070. PTHR24070. 1 hit.
PTHR24070:SF277. PTHR24070:SF277. 1 hit.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51421. RAS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS
60 70 80 90 100
YRKKVVLDGE EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES
110 120 130 140 150
FAATADFREQ ILRVKEDENV PFLLVGNKSD LEDKRQVSVE EAKNRADQWN
160 170 180 190 200
VNYVETSAKT RANVDKVFFD LMREIRARKM EDSKEKNGKK KRKSLAKRIR

ERCCIL
Length:206
Mass (Da):23,553
Last modified:October 11, 2004 - v1
Checksum:i6974341FA08C1874
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48587 mRNA. Translation: CAA88488.1.
AF244951 mRNA. Translation: AAG23136.1.
BC031741 mRNA. Translation: AAH31741.1.
CCDSiCCDS26255.1.
RefSeqiNP_062364.3. NM_019491.5.
UniGeneiMm.27348.

Genome annotation databases

EnsembliENSMUST00000009003; ENSMUSP00000009003; ENSMUSG00000008859.
GeneIDi56044.
KEGGimmu:56044.
UCSCiuc007pof.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRALA_MOUSE
AccessioniPrimary (citable) accession number: P63321
Secondary accession number(s): P05810
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 5, 2017
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families