Reviewed, UniProtKB/Swiss-Prot P63321 (RALA_MOUSE)

Names and origin

Protein names

Recommended name:
    Ras-related protein Ral-A

Gene names

Name:	Rala
Synonyms:	Ral, Ral-a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Interacts with CDC42 By similarity. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding By similarity. Interacts with RALGPS1 By similarity. Interacts with RALBP1 via its effector domain.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.5 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Ras-related protein Ral-A		PRO_0000082694
	Propeptide	204 – 206	3	Removed in mature form By similarity		PRO_0000281345
Regions
	Nucleotide binding	21 – 28	8	GTP By similarity
	Nucleotide binding	68 – 72	5	GTP By similarity
	Nucleotide binding	127 – 130	4	GTP By similarity
	Motif	43 – 51	9	Effector region By similarity
Amino acid modifications
	Modified residue	203	1	Cysteine methyl ester By similarity
	Lipidation	203	1	S-geranylgeranyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63321-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6974341FA08C1874	FASTA	206	23,553
	10 20 30 40 50 60 MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS YRKKVVLDGE 70 80 90 100 110 120 EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES FAATADFREQ ILRVKEDENV 130 140 150 160 170 180 PFLLVGNKSD LEDKRQVSVE EAKNRADQWN VNYVETSAKT RANVDKVFFD LMREIRARKM 190 200 EDSKEKNGKK KRKSLAKRIR ERCCIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Roger T.T. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Spleen.
[2]	"Tissue-specific expression of GTPas RalA and RalB during embryogenesis and regulation by epithelial-mesenchymal interaction." Zhao Z., Rivkees S.A. Mech. Dev. 97:201-204(2000) [PubMed: 11025226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 8-16; 28-47; 114-128 AND 167-173, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[5]	"A putative effector of Ral has homology to Rho/Rac GTPase activating proteins." Park S.-H., Weinberg R.A. Oncogene 11:2349-2355(1995) [PubMed: 8570186] [Abstract] Cited for: INTERACTION WITH RALBP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48587 mRNA. Translation: CAA88488.1. AF244951 mRNA. Translation: AAG23136.1. BC031741 mRNA. Translation: AAH31741.1.
IPI	IPI00124282.
RefSeq	NP_062364.3.
UniGene	Mm.27348
3D structure databases
HSSP	HSSP built from PDB template 1UAD based on UniProtKB P11233.
SMR	P63321. Positions 11-183.
ModBase	Search...
Protein-protein interaction databases
STRING	P63321.
PTM databases
PhosphoSite	P63321.
Proteomic databases
PRIDE	P63321.
Genome annotation databases
Ensembl	ENSMUST00000009003; ENSMUSP00000009003; ENSMUSG00000008859; Mus musculus. [Genome view]
GeneID	56044.
KEGG	mmu:56044.
UCSC	uc007pof.1. mouse.
Organism-specific databases
CTD	56044.
MGI	MGI:1927243. Rala.
Phylogenomic databases
HOGENOM	P63321.
HOVERGEN	P63321.
OMA	AKPKGQN.
Gene expression databases
ArrayExpress	P63321.
Bgee	P63321.
Genevestigator	P63321.
GermOnline	ENSMUSG00000008859. Mus musculus.
Family and domain databases
InterPro	IPR003577. GTPase_Ras. IPR013753. Ras. IPR001806. Ras_GTPase. IPR015591. Ras_Ral_related. IPR005225. Small_GTP_bd. [Graphical view]
PANTHER	PTHR11708:SF124. Ras_Ral_related. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	311812.
SOURCE	Search...

Entry information

Entry name

RALA_MOUSE

Accession

Primary (citable) accession number: P63321
Secondary accession number(s): P05810

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)