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Protein

SUMO-conjugating enzyme UBC9

Gene

UBE2I

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Essential for nuclear architecture and chromosome segregation.1 Publication

Catalytic activityi

ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Glycyl thioester intermediatePROSITE-ProRule annotation1
Sitei100 – 101Substrate bindingBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-3108214. SUMOylation of DNA damage response and repair proteins.
R-GGA-3232118. SUMOylation of transcription factors.
R-GGA-4551638. SUMOylation of chromatin organization proteins.
R-GGA-4570464. SUMOylation of RNA binding proteins.
R-GGA-4615885. SUMOylation of DNA replication proteins.
R-GGA-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-GGA-5693607. Processing of DNA double-strand break ends.
R-GGA-5696395. Formation of Incision Complex in GG-NER.
R-GGA-8866904. Negative regulation of activity of TFAP2 (AP-2) family transcription factors.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-conjugating enzyme UBC9 (EC:6.3.2.-)
Alternative name(s):
SUMO-protein ligase
Ubiquitin carrier protein 9
Ubiquitin carrier protein I
Ubiquitin-conjugating enzyme E2 I
Ubiquitin-protein ligase I
Gene namesi
Name:UBE2I
Synonyms:UBC9, UBCE9
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824591 – 158SUMO-conjugating enzyme UBC9Add BLAST158

Proteomic databases

PaxDbiP63283.
PRIDEiP63283.

Expressioni

Gene expression databases

BgeeiENSGALG00000006428.

Interactioni

Subunit structurei

Forms a tight complex with RANGAP1 and RANBP2 (By similarity). Interacts with SOX9.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei4Interaction with RANBP2By similarity1
Sitei25Interaction with RANBP2By similarity1
Sitei57Interaction with RANBP2By similarity1

GO - Molecular functioni

  • transcription factor binding Source: AgBase

Protein-protein interaction databases

BioGridi675156. 1 interactor.
STRINGi9031.ENSGALP00000039418.

Structurei

3D structure databases

ProteinModelPortaliP63283.
SMRiP63283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 18Interaction with SUMO1By similarity6

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0424. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00550000075088.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP63283.
KOiK10577.
OMAiDLKRWEC.
OrthoDBiEOG091G0NAK.
PhylomeDBiP63283.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR027230. Ubc9.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PANTHERiPTHR24067:SF51. PTHR24067:SF51. 1 hit.
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG
60 70 80 90 100
TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED
110 120 130 140 150
KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA

QAKKFAPS
Length:158
Mass (Da):18,007
Last modified:October 11, 2004 - v1
Checksum:iE2C826E9C8D0683D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB069964 mRNA. Translation: BAB68210.1.
AF461016 mRNA. Translation: AAL85282.1.
RefSeqiNP_989596.1. NM_204265.1.
XP_015149596.1. XM_015294110.1.
XP_015149597.1. XM_015294111.1.
XP_015149598.1. XM_015294112.1.
XP_015149599.1. XM_015294113.1.
XP_015149601.1. XM_015294115.1.
UniGeneiGga.4940.

Genome annotation databases

EnsembliENSGALT00000010377; ENSGALP00000010363; ENSGALG00000006428.
ENSGALT00000062070; ENSGALP00000045009; ENSGALG00000006428.
ENSGALT00000076663; ENSGALP00000048004; ENSGALG00000006428.
GeneIDi374123.
KEGGigga:374123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB069964 mRNA. Translation: BAB68210.1.
AF461016 mRNA. Translation: AAL85282.1.
RefSeqiNP_989596.1. NM_204265.1.
XP_015149596.1. XM_015294110.1.
XP_015149597.1. XM_015294111.1.
XP_015149598.1. XM_015294112.1.
XP_015149599.1. XM_015294113.1.
XP_015149601.1. XM_015294115.1.
UniGeneiGga.4940.

3D structure databases

ProteinModelPortaliP63283.
SMRiP63283.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi675156. 1 interactor.
STRINGi9031.ENSGALP00000039418.

Proteomic databases

PaxDbiP63283.
PRIDEiP63283.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000010377; ENSGALP00000010363; ENSGALG00000006428.
ENSGALT00000062070; ENSGALP00000045009; ENSGALG00000006428.
ENSGALT00000076663; ENSGALP00000048004; ENSGALG00000006428.
GeneIDi374123.
KEGGigga:374123.

Organism-specific databases

CTDi7329.

Phylogenomic databases

eggNOGiKOG0424. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00550000075088.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP63283.
KOiK10577.
OMAiDLKRWEC.
OrthoDBiEOG091G0NAK.
PhylomeDBiP63283.

Enzyme and pathway databases

UniPathwayiUPA00886.
ReactomeiR-GGA-3108214. SUMOylation of DNA damage response and repair proteins.
R-GGA-3232118. SUMOylation of transcription factors.
R-GGA-4551638. SUMOylation of chromatin organization proteins.
R-GGA-4570464. SUMOylation of RNA binding proteins.
R-GGA-4615885. SUMOylation of DNA replication proteins.
R-GGA-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-GGA-5693607. Processing of DNA double-strand break ends.
R-GGA-5696395. Formation of Incision Complex in GG-NER.
R-GGA-8866904. Negative regulation of activity of TFAP2 (AP-2) family transcription factors.

Miscellaneous databases

PROiP63283.

Gene expression databases

BgeeiENSGALG00000006428.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR027230. Ubc9.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PANTHERiPTHR24067:SF51. PTHR24067:SF51. 1 hit.
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC9_CHICK
AccessioniPrimary (citable) accession number: P63283
Secondary accession number(s): P50550
, Q15698, Q547R1, Q86VB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.