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Protein

Amelogenin, X isoform

Gene

Amelx

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in the biomineralization of teeth. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.

GO - Molecular functioni

  • identical protein binding Source: RGD
  • structural constituent of tooth enamel Source: RGD

GO - Biological processi

  • biomineral tissue development Source: UniProtKB-KW
  • response to calcium ion Source: RGD
  • response to drug Source: RGD
  • response to nutrient Source: RGD
Complete GO annotation...

Keywords - Biological processi

Biomineralization

Names & Taxonomyi

Protein namesi
Recommended name:
Amelogenin, X isoform
Alternative name(s):
Leucine-rich amelogenin peptide
Short name:
LRAP
Gene namesi
Name:Amelx
Synonyms:Amel, Amgx
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2107. Amelx.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • endocytic vesicle Source: RGD
  • extracellular matrix Source: RGD
  • Golgi apparatus Source: RGD
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616By similarityAdd
BLAST
Chaini17 – 210194Amelogenin, X isoformPRO_0000001202Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321PhosphoserineBy similarity

Post-translational modificationi

Several forms are produced by C-terminal processing.
Phosphorylated by FAM20C in vitro.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP63278.

Expressioni

Gene expression databases

ExpressionAtlasiP63278. baseline and differential.
GenevisibleiP63278. RN.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: RGD

Family & Domainsi

Sequence similaritiesi

Belongs to the amelogenin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00390000009151.
HOGENOMiHOG000231643.
HOVERGENiHBG016835.
InParanoidiP63278.
OMAiHSMTPTQ.
OrthoDBiEOG7JQBQ8.
PhylomeDBiP63278.
TreeFamiTF337092.

Family and domain databases

InterProiIPR004116. Amelogenin.
[Graphical view]
PfamiPF02948. Amelogenin. 1 hit.
[Graphical view]
PRINTSiPR01757. AMELOGENIN.
SMARTiSM00818. Amelogenin. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 4 (identifier: P63278-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTWILFACL LGAAFAMPLP PHPGSPGYIN LSYEKSHSQA INTDRTALVL
60 70 80 90 100
TPLKWYQSMI RQPYPSYGYE PMGGWLHHQI IPVLSQQHPP SHTLQPHHHL
110 120 130 140 150
PVVPAQQPVA PQQPMMPVPG HHSMTPTQHH QPNIPPSAQQ PFQQPFQPQA
160 170 180 190 200
IPPQSHQPMQ PQSPLHPMQP LAPQPPLPPL FSMQPLSPIL PELPLEAWPA
210
TDKTKREEVD
Length:210
Mass (Da):23,483
Last modified:October 11, 2004 - v1
Checksum:iEB6ED5D09AA83AFA
GO
Isoform 1 (identifier: P63278-1) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.

Show »
Length:196
Mass (Da):21,959
Checksum:i8E9DE372A13669F4
GO
Isoform 2 (identifier: P63278-2) [UniParc] [UniParc]FASTAAdd to basket

Also known as: LRAP

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
     64-184: Missing.

Show »
Length:75
Mass (Da):8,406
Checksum:i14D8D8D2CA9DD235
GO
Isoform 3 (identifier: P63278-3) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
     64-87: Missing.

Show »
Length:172
Mass (Da):19,176
Checksum:i0C571EDAFACC111C
GO
Isoform 5 (identifier: P63278-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
     210-210: D → AFSPMKWYQGTARHPLNMETTTEK

Show »
Length:219
Mass (Da):24,652
Checksum:iBD42AF2E7EEC1955
GO
Isoform 6 (identifier: P63278-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
     64-87: Missing.
     210-210: D → AFSPMKWYQGTARHPLNMETTTEK

Show »
Length:195
Mass (Da):21,868
Checksum:iFD1EC774937209A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641Y → H in AAB02691 (PubMed:8600184).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei35 – 4814Missing in isoform 1, isoform 2, isoform 3, isoform 5 and isoform 6. 2 PublicationsVSP_011689Add
BLAST
Alternative sequencei64 – 184121Missing in isoform 2. CuratedVSP_011690Add
BLAST
Alternative sequencei64 – 8724Missing in isoform 3 and isoform 6. 1 PublicationVSP_011691Add
BLAST
Alternative sequencei210 – 2101D → AFSPMKWYQGTARHPLNMET TTEK in isoform 5 and isoform 6. 1 PublicationVSP_011692

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01245 mRNA. Translation: AAA20491.1.
U07054 mRNA. Translation: AAA61964.1.
U67130 mRNA. Translation: AAB06753.1.
U51195 mRNA. Translation: AAB02691.1.
U60562 mRNA. Translation: AAB03481.1.
PIRiJC2391.
RefSeqiNP_001258002.1. NM_001271073.1. [P63278-6]
NP_001258003.1. NM_001271074.1. [P63278-5]
NP_001258004.1. NM_001271075.1.
NP_001258005.1. NM_001271076.1.
NP_001258006.1. NM_001271077.1. [P63278-1]
NP_001258007.1. NM_001271078.1. [P63278-2]
NP_062027.1. NM_019154.1. [P63278-4]
UniGeneiRn.11368.

Genome annotation databases

EnsembliENSRNOT00000005328; ENSRNOP00000005328; ENSRNOG00000003965. [P63278-4]
ENSRNOT00000052176; ENSRNOP00000048382; ENSRNOG00000003965. [P63278-5]
ENSRNOT00000078843; ENSRNOP00000069787; ENSRNOG00000003965. [P63278-1]
ENSRNOT00000087293; ENSRNOP00000075671; ENSRNOG00000003965. [P63278-4]
GeneIDi29160.
KEGGirno:29160.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01245 mRNA. Translation: AAA20491.1.
U07054 mRNA. Translation: AAA61964.1.
U67130 mRNA. Translation: AAB06753.1.
U51195 mRNA. Translation: AAB02691.1.
U60562 mRNA. Translation: AAB03481.1.
PIRiJC2391.
RefSeqiNP_001258002.1. NM_001271073.1. [P63278-6]
NP_001258003.1. NM_001271074.1. [P63278-5]
NP_001258004.1. NM_001271075.1.
NP_001258005.1. NM_001271076.1.
NP_001258006.1. NM_001271077.1. [P63278-1]
NP_001258007.1. NM_001271078.1. [P63278-2]
NP_062027.1. NM_019154.1. [P63278-4]
UniGeneiRn.11368.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP63278.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005328; ENSRNOP00000005328; ENSRNOG00000003965. [P63278-4]
ENSRNOT00000052176; ENSRNOP00000048382; ENSRNOG00000003965. [P63278-5]
ENSRNOT00000078843; ENSRNOP00000069787; ENSRNOG00000003965. [P63278-1]
ENSRNOT00000087293; ENSRNOP00000075671; ENSRNOG00000003965. [P63278-4]
GeneIDi29160.
KEGGirno:29160.

Organism-specific databases

CTDi265.
RGDi2107. Amelx.

Phylogenomic databases

GeneTreeiENSGT00390000009151.
HOGENOMiHOG000231643.
HOVERGENiHBG016835.
InParanoidiP63278.
OMAiHSMTPTQ.
OrthoDBiEOG7JQBQ8.
PhylomeDBiP63278.
TreeFamiTF337092.

Miscellaneous databases

PROiP63278.

Gene expression databases

ExpressionAtlasiP63278. baseline and differential.
GenevisibleiP63278. RN.

Family and domain databases

InterProiIPR004116. Amelogenin.
[Graphical view]
PfamiPF02948. Amelogenin. 1 hit.
[Graphical view]
PRINTSiPR01757. AMELOGENIN.
SMARTiSM00818. Amelogenin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and DNA sequence of rat amelogenin and a comparative analysis of mammalian amelogenin protein sequence divergence."
    Bonass W.A., Robinson P.A., Kirkham J., Shore R.C., Robinson C.
    Biochem. Biophys. Res. Commun. 198:755-763(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Enamel organ.
  2. "Isolation and characterisation of an alternatively-spliced rat amelogenin cDNA: LRAP -- a highly conserved, functional alternatively-spliced amelogenin?"
    Bonass W.A., Kirkham J., Brookes S.J., Shore R.C., Robinson C.
    Biochim. Biophys. Acta 1219:690-692(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Wistar.
    Tissue: Enamel organ.
  3. "Rat amelogenin cDNA containing exon 4."
    Bonass W.A.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
  4. "Alternative splicing of amelogenin mRNA from rat incisor ameloblasts."
    Li R., Li W., DenBesten P.K.
    J. Dent. Res. 74:1880-1885(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5 AND 6).
    Tissue: Tooth enamel.

Entry informationi

Entry nameiAMELX_RAT
AccessioniPrimary (citable) accession number: P63278
Secondary accession number(s): P45559
, Q62945, Q63640, Q78E56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.