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Protein

Amelogenin, X isoform

Gene

Amelx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in the biomineralization of teeth. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.

GO - Molecular functioni

  1. growth factor activity Source: BHF-UCL
  2. hydroxyapatite binding Source: BHF-UCL
  3. identical protein binding Source: BHF-UCL
  4. structural constituent of tooth enamel Source: MGI

GO - Biological processi

  1. cell adhesion Source: BHF-UCL
  2. cell proliferation Source: BHF-UCL
  3. odontogenesis of dentin-containing tooth Source: BHF-UCL
  4. signal transduction Source: BHF-UCL
  5. tooth mineralization Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Biomineralization

Names & Taxonomyi

Protein namesi
Recommended name:
Amelogenin, X isoform
Alternative name(s):
Leucine-rich amelogenin peptide
Short name:
LRAP
Gene namesi
Name:Amelx
Synonyms:Amel
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88005. Amelx.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: MGI
  2. cell surface Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616By similarityAdd
BLAST
Chaini17 – 210194Amelogenin, X isoformPRO_0000001201Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321PhosphoserineBy similarity

Post-translational modificationi

Several forms are produced by C-terminal processing.

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

BgeeiP63277.
CleanExiMM_AMELX.
ExpressionAtlasiP63277. baseline and differential.
GenevestigatoriP63277.

Interactioni

Protein-protein interaction databases

DIPiDIP-59703N.

Structurei

3D structure databases

DisProtiDP00692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amelogenin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG69988.
GeneTreeiENSGT00390000009151.
HOGENOMiHOG000231643.
HOVERGENiHBG016835.
InParanoidiP63277.

Family and domain databases

InterProiIPR004116. Amelogenin.
[Graphical view]
PfamiPF02948. Amelogenin. 1 hit.
[Graphical view]
PRINTSiPR01757. AMELOGENIN.
SMARTiSM00818. Amelogenin. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 4 (identifier: P63277-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTWILFACL LGAAFAMPLP PHPGSPGYIN LSYEKSHSQA INTDRTALVL
60 70 80 90 100
TPLKWYQSMI RQPYPSYGYE PMGGWLHHQI IPVLSQQHPP SHTLQPHHHL
110 120 130 140 150
PVVPAQQPVA PQQPMMPVPG HHSMTPTQHH QPNIPPSAQQ PFQQPFQPQA
160 170 180 190 200
IPPQSHQPMQ PQSPLHPMQP LAPQPPLPPL FSMQPLSPIL PELPLEAWPA
210
TDKTKREEVD
Length:210
Mass (Da):23,483
Last modified:October 10, 2004 - v1
Checksum:iEB6ED5D09AA83AFA
GO
Isoform 1 (identifier: P63277-1) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.

Show »
Length:196
Mass (Da):21,959
Checksum:i8E9DE372A13669F4
GO
Isoform 2 (identifier: P63277-2) [UniParc] [UniParc]FASTAAdd to basket

Also known as: LRAP

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
     64-184: Missing.

Show »
Length:75
Mass (Da):8,406
Checksum:i14D8D8D2CA9DD235
GO
Isoform 3 (identifier: P63277-3) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
     64-87: Missing.

Show »
Length:172
Mass (Da):19,176
Checksum:i0C571EDAFACC111C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111P → A in AAA37218 (PubMed:4015654).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei35 – 4814Missing in isoform 1, isoform 2 and isoform 3. 1 PublicationVSP_011688Add
BLAST
Alternative sequencei64 – 184121Missing in isoform 2. CuratedVSP_000230Add
BLAST
Alternative sequencei64 – 8724Missing in isoform 3. CuratedVSP_000231Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31768 mRNA. Translation: BAA06546.1.
D31769 mRNA. Translation: BAA06547.1.
D83067 Genomic DNA. Translation: BAA23665.1.
AK029358 mRNA. Translation: BAC26415.1.
AL805974 Genomic DNA. Translation: CAM21303.1.
AL805974 Genomic DNA. Translation: CAM21304.1.
M10095 mRNA. Translation: AAA37218.1.
CCDSiCCDS41214.1. [P63277-1]
PIRiI49486.
PC1148.
RefSeqiNP_001075447.1. NM_001081978.2.
NP_001277300.1. NM_001290371.1. [P63277-3]
NP_033796.1. NM_009666.4. [P63277-1]
XP_006528755.1. XM_006528692.2. [P63277-4]
UniGeneiMm.391342.

Genome annotation databases

EnsembliENSMUST00000112118; ENSMUSP00000107746; ENSMUSG00000031354. [P63277-1]
ENSMUST00000112119; ENSMUSP00000107747; ENSMUSG00000031354. [P63277-4]
ENSMUST00000112120; ENSMUSP00000107748; ENSMUSG00000031354. [P63277-3]
GeneIDi11704.
KEGGimmu:11704.
UCSCiuc009uxt.2. mouse. [P63277-3]
uc009uxu.1. mouse. [P63277-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31768 mRNA. Translation: BAA06546.1.
D31769 mRNA. Translation: BAA06547.1.
D83067 Genomic DNA. Translation: BAA23665.1.
AK029358 mRNA. Translation: BAC26415.1.
AL805974 Genomic DNA. Translation: CAM21303.1.
AL805974 Genomic DNA. Translation: CAM21304.1.
M10095 mRNA. Translation: AAA37218.1.
CCDSiCCDS41214.1. [P63277-1]
PIRiI49486.
PC1148.
RefSeqiNP_001075447.1. NM_001081978.2.
NP_001277300.1. NM_001290371.1. [P63277-3]
NP_033796.1. NM_009666.4. [P63277-1]
XP_006528755.1. XM_006528692.2. [P63277-4]
UniGeneiMm.391342.

3D structure databases

DisProtiDP00692.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59703N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000112118; ENSMUSP00000107746; ENSMUSG00000031354. [P63277-1]
ENSMUST00000112119; ENSMUSP00000107747; ENSMUSG00000031354. [P63277-4]
ENSMUST00000112120; ENSMUSP00000107748; ENSMUSG00000031354. [P63277-3]
GeneIDi11704.
KEGGimmu:11704.
UCSCiuc009uxt.2. mouse. [P63277-3]
uc009uxu.1. mouse. [P63277-4]

Organism-specific databases

CTDi265.
MGIiMGI:88005. Amelx.

Phylogenomic databases

eggNOGiNOG69988.
GeneTreeiENSGT00390000009151.
HOGENOMiHOG000231643.
HOVERGENiHBG016835.
InParanoidiP63277.

Miscellaneous databases

NextBioi279383.
PROiP63277.
SOURCEiSearch...

Gene expression databases

BgeeiP63277.
CleanExiMM_AMELX.
ExpressionAtlasiP63277. baseline and differential.
GenevestigatoriP63277.

Family and domain databases

InterProiIPR004116. Amelogenin.
[Graphical view]
PfamiPF02948. Amelogenin. 1 hit.
[Graphical view]
PRINTSiPR01757. AMELOGENIN.
SMARTiSM00818. Amelogenin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Alternative splicing of the mouse amelogenin primary RNA transcript contributes to amelogenin heterogeneity."
    Lau E.C., Simmer J.P., Bringas P. Jr., Hsu D.D.J., Hu C.C., Zeichner-David M., Thiemann F., Snead M.L., Slavkin H.C., Fincham A.G.
    Biochem. Biophys. Res. Commun. 188:1253-1260(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2 AND 3).
    Strain: ICR.
  2. Oida S., Iimura T., Arai N., Takeda K., Maruoka Y., Terashima T., Shimokawa H., Sasaki S.
    Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 4.
  3. "Molecular structure of the mouse amelogenin genomic DNA."
    Oida S., Miyazaki H., Iimura T., Suzuki M., Sasaki M., Shimokawa H.
    DNA Seq. 6:307-310(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 4).
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Head.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "DNA sequence for cloned cDNA for murine amelogenin reveal the amino acid sequence for enamel-specific protein."
    Snead M.L., Lau E.C., Zeichner-David M., Fincham A.G., Woo S.L., Slavkin H.C.
    Biochem. Biophys. Res. Commun. 129:812-818(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-210.

Entry informationi

Entry nameiAMELX_MOUSE
AccessioniPrimary (citable) accession number: P63277
Secondary accession number(s): A2ALX2
, A2ALX3, P45559, P70592, Q61293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2004
Last sequence update: October 10, 2004
Last modified: March 31, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.