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P63277 (AMELX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amelogenin, X isoform
Alternative name(s):
Leucine-rich amelogenin peptide
Short name=LRAP
Gene names
Name:Amelx
Synonyms:Amel
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in the biomineralization of teeth. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel.

Subcellular location

Secretedextracellular spaceextracellular matrix.

Post-translational modification

Several forms are produced by C-terminal processing.

Sequence similarities

Belongs to the amelogenin family.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 4 (identifier: P63277-4)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: P63277-1)

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
Isoform 2 (identifier: P63277-2)

Also known as: LRAP;

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
     64-184: Missing.
Isoform 3 (identifier: P63277-3)

The sequence of this isoform differs from the canonical sequence as follows:
     35-48: Missing.
     64-87: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 By similarity
Chain17 – 210194Amelogenin, X isoform
PRO_0000001201

Amino acid modifications

Modified residue321Phosphoserine By similarity

Natural variations

Alternative sequence35 – 4814Missing in isoform 1, isoform 2 and isoform 3.
VSP_011688
Alternative sequence64 – 184121Missing in isoform 2.
VSP_000230
Alternative sequence64 – 8724Missing in isoform 3.
VSP_000231

Experimental info

Sequence conflict1111P → A in AAA37218. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 4 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: EB6ED5D09AA83AFA

FASTA21023,483
        10         20         30         40         50         60 
MGTWILFACL LGAAFAMPLP PHPGSPGYIN LSYEKSHSQA INTDRTALVL TPLKWYQSMI 

        70         80         90        100        110        120 
RQPYPSYGYE PMGGWLHHQI IPVLSQQHPP SHTLQPHHHL PVVPAQQPVA PQQPMMPVPG 

       130        140        150        160        170        180 
HHSMTPTQHH QPNIPPSAQQ PFQQPFQPQA IPPQSHQPMQ PQSPLHPMQP LAPQPPLPPL 

       190        200        210 
FSMQPLSPIL PELPLEAWPA TDKTKREEVD 

« Hide

Isoform 1 [UniParc] [UniParc].

Checksum: 8E9DE372A13669F4
Show »

FASTA19621,959
Isoform 2 (LRAP) [UniParc] [UniParc].

Checksum: 14D8D8D2CA9DD235
Show »

FASTA758,406
Isoform 3 [UniParc] [UniParc].

Checksum: 0C571EDAFACC111C
Show »

FASTA17219,176

References

« Hide 'large scale' references
[1]"Alternative splicing of the mouse amelogenin primary RNA transcript contributes to amelogenin heterogeneity."
Lau E.C., Simmer J.P., Bringas P. Jr., Hsu D.D.J., Hu C.C., Zeichner-David M., Thiemann F., Snead M.L., Slavkin H.C., Fincham A.G.
Biochem. Biophys. Res. Commun. 188:1253-1260(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2 AND 3).
Strain: ICR.
[2]Oida S., Iimura T., Arai N., Takeda K., Maruoka Y., Terashima T., Shimokawa H., Sasaki S.
Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 4.
[3]"Molecular structure of the mouse amelogenin genomic DNA."
Oida S., Miyazaki H., Iimura T., Suzuki M., Sasaki M., Shimokawa H.
DNA Seq. 6:307-310(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 4).
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Head.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]"DNA sequence for cloned cDNA for murine amelogenin reveal the amino acid sequence for enamel-specific protein."
Snead M.L., Lau E.C., Zeichner-David M., Fincham A.G., Woo S.L., Slavkin H.C.
Biochem. Biophys. Res. Commun. 129:812-818(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-210.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D31768 mRNA. Translation: BAA06546.1.
D31769 mRNA. Translation: BAA06547.1.
D83067 Genomic DNA. Translation: BAA23665.1.
AK029358 mRNA. Translation: BAC26415.1.
AL805974 Genomic DNA. Translation: CAM21303.1.
AL805974 Genomic DNA. Translation: CAM21304.1.
M10095 mRNA. Translation: AAA37218.1.
CCDSCCDS41214.1. [P63277-1]
PIRI49486.
PC1148.
RefSeqNP_001075447.1. NM_001081978.2.
NP_001277300.1. NM_001290371.1. [P63277-3]
NP_033796.1. NM_009666.4. [P63277-1]
XP_006528755.1. XM_006528692.1. [P63277-4]
UniGeneMm.391342.

3D structure databases

DisProtDP00692.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59703N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000112118; ENSMUSP00000107746; ENSMUSG00000031354. [P63277-1]
ENSMUST00000112119; ENSMUSP00000107747; ENSMUSG00000031354. [P63277-4]
ENSMUST00000112120; ENSMUSP00000107748; ENSMUSG00000031354. [P63277-3]
GeneID11704.
KEGGmmu:11704.
UCSCuc009uxt.2. mouse. [P63277-3]
uc009uxu.1. mouse. [P63277-4]

Organism-specific databases

CTD265.
MGIMGI:88005. Amelx.

Phylogenomic databases

eggNOGNOG69988.
GeneTreeENSGT00390000009151.
HOGENOMHOG000231643.
HOVERGENHBG016835.

Gene expression databases

BgeeP63277.
CleanExMM_AMELX.
GenevestigatorP63277.

Family and domain databases

InterProIPR004116. Amelogenin.
[Graphical view]
PfamPF02948. Amelogenin. 1 hit.
[Graphical view]
PRINTSPR01757. AMELOGENIN.
SMARTSM00818. Amelogenin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279383.
PROP63277.
SOURCESearch...

Entry information

Entry nameAMELX_MOUSE
AccessionPrimary (citable) accession number: P63277
Secondary accession number(s): A2ALX2 expand/collapse secondary AC list , A2ALX3, P45559, P70592, Q61293
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot