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Protein

Transcription elongation factor SPT4-A

Gene

Supt4h1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 36C4-typeSequence analysisAdd BLAST21

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-6807505 RNA polymerase II transcribes snRNA genes
R-MMU-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT4-A
Alternative name(s):
DRB sensitivity-inducing factor small subunit 1
Short name:
DSIF small subunit 1
Transcription elongation factor SPT4 1
Gene namesi
Name:Supt4h1a
Synonyms:Spt4h, Supt4a, Supt4h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107416 Supt4a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002103272 – 117Transcription elongation factor SPT4-AAdd BLAST116

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP63271
PaxDbiP63271
PeptideAtlasiP63271
PRIDEiP63271

PTM databases

iPTMnetiP63271
PhosphoSitePlusiP63271

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

BgeeiENSMUSG00000020485
CleanExiMM_SUPT4H1
ExpressionAtlasiP63271 baseline and differential
GenevisibleiP63271 MM

Interactioni

Subunit structurei

Interacts with SUPT5H to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of WHSC2/NELFA, COBRA1/NELFB, TH1L/NELFD and RDBP/NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II. DSIF also interacts with HRMT1L2/PRMT1, HTATSF1/TATSF1, RNGTT/CAP1A, SKB1/PRMT5, SUPT6H, and can interact with PIN1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203573, 2 interactors
STRINGi10090.ENSMUSP00000091487

Structurei

3D structure databases

ProteinModelPortaliP63271
SMRiP63271
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 40Interaction with SUPT5HBy similarityAdd BLAST39

Sequence similaritiesi

Belongs to the SPT4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 36C4-typeSequence analysisAdd BLAST21

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3490 Eukaryota
COG5204 LUCA
GeneTreeiENSGT00390000018559
HOVERGENiHBG055988
InParanoidiP63271
KOiK15171
OMAiVRGMYAV
OrthoDBiEOG091G0NIU
PhylomeDBiP63271
TreeFamiTF300105

Family and domain databases

CDDicd07973 Spt4, 1 hit
Gene3Di3.30.40.210, 1 hit
InterProiView protein in InterPro
IPR029040 RPABC4/Spt4
IPR009287 Spt4
IPR022800 Spt4/RpoE2_Znf
IPR038510 Spt4_sf
PANTHERiPTHR12882 PTHR12882, 1 hit
PfamiView protein in Pfam
PF06093 Spt4, 1 hit
PIRSFiPIRSF025023 Spt4, 1 hit
SMARTiView protein in SMART
SM01389 Spt4, 1 hit
SUPFAMiSSF63393 SSF63393, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63271-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALETVPKDL RHLRACLLCS LVKTIDQFEY DGCDNCDAYL QMKGNREMVY
60 70 80 90 100
DCTSSSFDGI IAMMSPEDSW VSKWQRVSNF KPGVYAVSVT GRLPQGIVRE
110
LKSRGVAYKS RDTAIKT
Length:117
Mass (Da):13,193
Last modified:October 11, 2004 - v1
Checksum:i09EDF007501D0F03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11R → L in BAE39944 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43154 mRNA Translation: AAB18730.1
U96809 Genomic DNA Translation: AAC71659.1
AK167941 mRNA Translation: BAE39944.1
AL604022 Genomic DNA Translation: CAI35953.1
CU393486 Genomic DNA Translation: CAQ52074.1
CH466556 Genomic DNA Translation: EDL15830.1
BC024391 mRNA Translation: AAH24391.1
BC061174 mRNA Translation: AAH61174.1
BC087923 mRNA Translation: AAH87923.1
BC141092 mRNA Translation: AAI41093.1
CCDSiCCDS36270.1
RefSeqiNP_033322.1, NM_009296.1
UniGeneiMm.622

Genome annotation databases

EnsembliENSMUST00000093955; ENSMUSP00000091487; ENSMUSG00000020485
GeneIDi20922
KEGGimmu:20922
UCSCiuc007kuj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSPT4A_MOUSE
AccessioniPrimary (citable) accession number: P63271
Secondary accession number(s): B2KGH6
, Q16550, Q3TIA2, Q5NCP8, Q5NCP9, Q5NCQ0, Q62387
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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