P63259 (ACTG_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actin, cytoplasmic 2

Alternative name(s):
Gamma-actin

Cleaved into the following chain:

1. Actin, cytoplasmic 2, N-terminally processed

Gene names

Name:	Actg1
Synonyms:	Actg

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit structure	Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Sequence similarities	Belongs to the actin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
PTM	Acetylation Methylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	response to calcium ion Inferred from expression pattern. Source: RGD
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Actin, cytoplasmic 2		PRO_0000367102
	Initiator methionine	1	1	Removed; alternate By similarity
	Chain	2 – 375	374	Actin, cytoplasmic 2, N-terminally processed		PRO_0000000835
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine; in Actin, cytoplasmic 2; alternate By similarity
	Modified residue	2	1	N-acetylglutamate; in Actin, cytoplasmic 2, N-terminally processed By similarity
	Modified residue	73	1	Tele-methylhistidine By similarity
	Modified residue	169	1	Phosphotyrosine By similarity
	Modified residue	198	1	Phosphotyrosine By similarity
	Modified residue	294	1	Phosphotyrosine By similarity
	Modified residue	318	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63259 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 54D08F986964EFD5	FASTA	375	41,793
	10 20 30 40 50 60 MEEEIAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS 70 80 90 100 110 120 KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT 130 140 150 160 170 180 QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL 190 200 210 220 230 240 AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY 250 260 270 280 290 300 ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS 310 320 330 340 350 360 GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ 370 EYDESGPSIV HRKCF

References

[1]	"A cDNA sequence encoding cytoskeletal gamma-actin from rat." Brown C.W., McHugh K.M., Lessard J.L. Nucleic Acids Res. 18:5312-5312(1990) [PubMed: 2402472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"A microsequencing approach to identify proteins which appear to interact with thyrotropin in rat FRTL-5 thyroid cells." Akamizu T., Saji M., Kohn L.D. Biochem. Biophys. Res. Commun. 170:351-358(1990) [PubMed: 2372296] [Abstract] Cited for: PROTEIN SEQUENCE OF 4-19 AND 228-231.
[3]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 29-37; 51-62 AND 85-95, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52815 mRNA. Translation: CAA36999.1.
IPI	IPI00896224.
PIR	ATRTC. A38571. S11222.
RefSeq	NP_001120921.1. NM_001127449.1. XP_002729242.1. XM_002729196.1.
UniGene	Rn.101464. Rn.106826.
3D structure databases
ProteinModelPortal	P63259.
SMR	P63259. Positions 2-375.
ModBase	Search...
Protein-protein interaction databases
IntAct	P63259. 3 interactions.
STRING	P63259.
PTM databases
PhosphoSite	P63259.
2D gel databases
World-2DPAGE	0004:P63259.
Proteomic databases
PRIDE	P63259.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000054976; ENSRNOP00000051859; ENSRNOG00000036701.
GeneID	100361457. 287876.
KEGG	rno:100361457. rno:287876.
Organism-specific databases
CTD	71.
RGD	1304556. Actg1.
Phylogenomic databases
HOVERGEN	HBG003771.
InParanoid	P63259.
OMA	IAMEEEI.
OrthoDB	EOG41JZC9.
PhylomeDB	P63259.
Enzyme and pathway databases
Reactome	REACT_114732. Cell-Cell communication.
Gene expression databases
Genevestigator	P63259.
GermOnline	ENSRNOG00000036701. Rattus norvegicus.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR020902. Actin/actin-like_CS. IPR004001. Actin_CS. [Graphical view]
KO	K05692.
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS00406. ACTINS_1. 1 hit. PS00432. ACTINS_2. 1 hit. PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	627161.

Entry information

Entry name

ACTG_RAT

Accession

Primary (citable) accession number: P63259
Secondary accession number(s): P02571, P14104, P99022

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program