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Protein

G protein-activated inward rectifier potassium channel 1

Gene

Kcnj3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei173Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • potassium ion import Source: GO_Central
  • response to electrical stimulus Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 1
Short name:
GIRK-1
Alternative name(s):
Inward rectifier K(+) channel Kir3.1
KGA
KGB1
Potassium channel, inwardly rectifying subfamily J member 3
Gene namesi
Name:Kcnj3
Synonyms:Girk1, Kga
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2958. Kcnj3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 80CytoplasmicAdd BLAST80
Transmembranei81 – 105Helical; Name=M1By similarityAdd BLAST25
Topological domaini106 – 129ExtracellularBy similarityAdd BLAST24
Intramembranei130 – 141Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei142 – 148Pore-formingBy similarity7
Topological domaini149 – 157ExtracellularBy similarity9
Transmembranei158 – 179Helical; Name=M2By similarityAdd BLAST22
Topological domaini180 – 501CytoplasmicAdd BLAST322

GO - Cellular componenti

  • cell surface Source: RGD
  • external side of plasma membrane Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi434.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549401 – 501G protein-activated inward rectifier potassium channel 1Add BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Modified residuei385PhosphoserineBy similarity1
Modified residuei424PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP63251.
PRIDEiP63251.

PTM databases

iPTMnetiP63251.
PhosphoSitePlusiP63251.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005369.
GenevisibleiP63251. RN.

Interactioni

Subunit structurei

Associates with GIRK2, GIRK3 or GIRK4 to form a G-protein activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKACAP005176EBI-7250981,EBI-7251007From a different organism.

Protein-protein interaction databases

IntActiP63251. 1 interactor.
MINTiMINT-7260243.
STRINGi10116.ENSRNOP00000007335.

Chemistry databases

BindingDBiP63251.

Structurei

Secondary structure

1501
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi200 – 203Combined sources4
Beta strandi208 – 211Combined sources4
Beta strandi213 – 215Combined sources3
Beta strandi219 – 221Combined sources3
Beta strandi224 – 237Combined sources14
Beta strandi243 – 250Combined sources8
Turni255 – 257Combined sources3
Beta strandi261 – 263Combined sources3
Beta strandi268 – 273Combined sources6
Turni279 – 282Combined sources4
Beta strandi294 – 303Combined sources10
Turni304 – 306Combined sources3
Beta strandi312 – 317Combined sources6
Helixi318 – 320Combined sources3
Beta strandi321 – 323Combined sources3
Beta strandi325 – 327Combined sources3
Beta strandi331 – 336Combined sources6
Beta strandi338 – 340Combined sources3
Helixi342 – 344Combined sources3
Beta strandi348 – 350Combined sources3
Helixi358 – 363Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N9PX-ray1.80A190-371[»]
ProteinModelPortaliP63251.
SMRiP63251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi143 – 148Selectivity filterBy similarity6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP63251.
KOiK04997.
OMAiNQPPPEK.
OrthoDBiEOG091G08HC.
PhylomeDBiP63251.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003274. K_chnl_inward-rec_Kir3.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PRINTSiPR01327. KIR31CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P63251-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALRRKFGD DYQVVTTSSS GSGLQPQGPG QGPQQQLVPK KKRQRFVDKN
60 70 80 90 100
GRCNVQHGNL GSETSRYLSD LFTTLVDLKW RWNLFIFILT YTVAWLFMAS
110 120 130 140 150
MWWVIAYTRG DLNKAHVGNY TPCVANVYNF PSAFLFFIET EATIGYGYRY
160 170 180 190 200
ITDKCPEGII LFLFQSILGS IVDAFLIGCM FIKMSQPKKR AETLMFSEHA
210 220 230 240 250
VISMRDGKLT LMFRVGNLRN SHMVSAQIRC KLLKSRQTPE GEFLPLDQLE
260 270 280 290 300
LDVGFSTGAD QLFLVSPLTI CHVIDAKSPF YDLSQRSMQT EQFEVVVILE
310 320 330 340 350
GIVETTGMTC QARTSYTEDE VLWGHRFFPV ISLEEGFFKV DYSQFHATFE
360 370 380 390 400
VPTPPYSVKE QEEMLLMSSP LIAPAITNSK ERHNSVECLD GLDDISTKLP
410 420 430 440 450
SKLQKITGRE DFPKKLLRMS STTSEKAYSL GDLPMKLQRI SSVPGNSEEK
460 470 480 490 500
LVSKTTKMLS DPMSQSVADL PPKLQKMAGG PTRMEGNLPA KLRKMNSDRF

T
Length:501
Mass (Da):56,573
Last modified:October 11, 2004 - v1
Checksum:iAB8910E9CC08FFEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25264 mRNA. Translation: AAA41226.1.
U01071 mRNA. Translation: AAC52127.1.
U01141 mRNA. Translation: AAC52125.1.
Y12259 mRNA. Translation: CAA72936.1.
U09243 mRNA. Translation: AAA18031.1.
PIRiI61084.
RefSeqiNP_113798.1. NM_031610.3.
UniGeneiRn.9809.

Genome annotation databases

EnsembliENSRNOT00000007335; ENSRNOP00000007335; ENSRNOG00000005369.
GeneIDi50599.
KEGGirno:50599.
UCSCiRGD:2958. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25264 mRNA. Translation: AAA41226.1.
U01071 mRNA. Translation: AAC52127.1.
U01141 mRNA. Translation: AAC52125.1.
Y12259 mRNA. Translation: CAA72936.1.
U09243 mRNA. Translation: AAA18031.1.
PIRiI61084.
RefSeqiNP_113798.1. NM_031610.3.
UniGeneiRn.9809.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N9PX-ray1.80A190-371[»]
ProteinModelPortaliP63251.
SMRiP63251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP63251. 1 interactor.
MINTiMINT-7260243.
STRINGi10116.ENSRNOP00000007335.

Chemistry databases

BindingDBiP63251.
GuidetoPHARMACOLOGYi434.

PTM databases

iPTMnetiP63251.
PhosphoSitePlusiP63251.

Proteomic databases

PaxDbiP63251.
PRIDEiP63251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007335; ENSRNOP00000007335; ENSRNOG00000005369.
GeneIDi50599.
KEGGirno:50599.
UCSCiRGD:2958. rat.

Organism-specific databases

CTDi3760.
RGDi2958. Kcnj3.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP63251.
KOiK04997.
OMAiNQPPPEK.
OrthoDBiEOG091G08HC.
PhylomeDBiP63251.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

PROiP63251.

Gene expression databases

BgeeiENSRNOG00000005369.
GenevisibleiP63251. RN.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003274. K_chnl_inward-rec_Kir3.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PRINTSiPR01327. KIR31CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNJ3_RAT
AccessioniPrimary (citable) accession number: P63251
Secondary accession number(s): P35562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.