Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P63251

- KCNJ3_RAT

UniProt

P63251 - KCNJ3_RAT

Protein

G protein-activated inward rectifier potassium channel 1

Gene

Kcnj3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei173 – 1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. G-protein activated inward rectifier potassium channel activity Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. response to electrical stimulus Source: RGD

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-activated inward rectifier potassium channel 1
    Short name:
    GIRK-1
    Alternative name(s):
    Inward rectifier K(+) channel Kir3.1
    KGA
    KGB1
    Potassium channel, inwardly rectifying subfamily J member 3
    Gene namesi
    Name:Kcnj3
    Synonyms:Girk1, Kga
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi2958. Kcnj3.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: RGD
    2. external side of plasma membrane Source: RGD
    3. integral component of membrane Source: UniProtKB-KW
    4. T-tubule Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501G protein-activated inward rectifier potassium channel 1PRO_0000154940Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP63251.

    Expressioni

    Gene expression databases

    GenevestigatoriP63251.

    Interactioni

    Subunit structurei

    Associates with GIRK2, GIRK3 or GIRK4 to form a G-protein activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRKACAP005176EBI-7250981,EBI-7251007From a different organism.

    Protein-protein interaction databases

    IntActiP63251. 1 interaction.
    MINTiMINT-7260243.

    Structurei

    3D structure databases

    ProteinModelPortaliP63251.
    SMRiP63251. Positions 41-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8080CytoplasmicAdd
    BLAST
    Topological domaini106 – 12924ExtracellularBy similarityAdd
    BLAST
    Topological domaini149 – 1579ExtracellularBy similarity
    Topological domaini180 – 501322CytoplasmicAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei130 – 14112Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei142 – 1487Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei81 – 10525Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei158 – 17922Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi143 – 1486Selectivity filterBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG280776.
    GeneTreeiENSGT00730000110555.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiP63251.
    KOiK04997.
    OMAiSMQSEQF.
    OrthoDBiEOG7XPZ5K.
    PhylomeDBiP63251.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003274. K_chnl_inward-rec_Kir3.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01327. KIR31CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P63251-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALRRKFGD DYQVVTTSSS GSGLQPQGPG QGPQQQLVPK KKRQRFVDKN    50
    GRCNVQHGNL GSETSRYLSD LFTTLVDLKW RWNLFIFILT YTVAWLFMAS 100
    MWWVIAYTRG DLNKAHVGNY TPCVANVYNF PSAFLFFIET EATIGYGYRY 150
    ITDKCPEGII LFLFQSILGS IVDAFLIGCM FIKMSQPKKR AETLMFSEHA 200
    VISMRDGKLT LMFRVGNLRN SHMVSAQIRC KLLKSRQTPE GEFLPLDQLE 250
    LDVGFSTGAD QLFLVSPLTI CHVIDAKSPF YDLSQRSMQT EQFEVVVILE 300
    GIVETTGMTC QARTSYTEDE VLWGHRFFPV ISLEEGFFKV DYSQFHATFE 350
    VPTPPYSVKE QEEMLLMSSP LIAPAITNSK ERHNSVECLD GLDDISTKLP 400
    SKLQKITGRE DFPKKLLRMS STTSEKAYSL GDLPMKLQRI SSVPGNSEEK 450
    LVSKTTKMLS DPMSQSVADL PPKLQKMAGG PTRMEGNLPA KLRKMNSDRF 500
    T 501
    Length:501
    Mass (Da):56,573
    Last modified:October 11, 2004 - v1
    Checksum:iAB8910E9CC08FFEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25264 mRNA. Translation: AAA41226.1.
    U01071 mRNA. Translation: AAC52127.1.
    U01141 mRNA. Translation: AAC52125.1.
    Y12259 mRNA. Translation: CAA72936.1.
    U09243 mRNA. Translation: AAA18031.1.
    PIRiI61084.
    RefSeqiNP_113798.1. NM_031610.3.
    UniGeneiRn.9809.

    Genome annotation databases

    EnsembliENSRNOT00000007335; ENSRNOP00000007335; ENSRNOG00000005369.
    GeneIDi50599.
    KEGGirno:50599.
    UCSCiRGD:2958. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25264 mRNA. Translation: AAA41226.1 .
    U01071 mRNA. Translation: AAC52127.1 .
    U01141 mRNA. Translation: AAC52125.1 .
    Y12259 mRNA. Translation: CAA72936.1 .
    U09243 mRNA. Translation: AAA18031.1 .
    PIRi I61084.
    RefSeqi NP_113798.1. NM_031610.3.
    UniGenei Rn.9809.

    3D structure databases

    ProteinModelPortali P63251.
    SMRi P63251. Positions 41-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P63251. 1 interaction.
    MINTi MINT-7260243.

    Chemistry

    BindingDBi P63251.
    GuidetoPHARMACOLOGYi 434.

    Proteomic databases

    PRIDEi P63251.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000007335 ; ENSRNOP00000007335 ; ENSRNOG00000005369 .
    GeneIDi 50599.
    KEGGi rno:50599.
    UCSCi RGD:2958. rat.

    Organism-specific databases

    CTDi 3760.
    RGDi 2958. Kcnj3.

    Phylogenomic databases

    eggNOGi NOG280776.
    GeneTreei ENSGT00730000110555.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi P63251.
    KOi K04997.
    OMAi SMQSEQF.
    OrthoDBi EOG7XPZ5K.
    PhylomeDBi P63251.
    TreeFami TF313676.

    Miscellaneous databases

    NextBioi 289889.
    PROi P63251.

    Gene expression databases

    Genevestigatori P63251.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003274. K_chnl_inward-rec_Kir3.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01327. KIR31CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain and Heart atrium.
    2. "Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel."
      Kubo Y., Reuveny E., Slesinger P.A., Jan Y.N., Jan L.Y.
      Nature 364:802-806(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "G protein-activated inwardly rectifying potassium channel (GIRK1/KGA) mRNA in adult rat heart and brain by in situ hybridization histochemistry."
      Depaoli A.M., Bell G.I., Stoffel M.
      Mol. Cell. Neurosci. 5:515-522(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "IRK(1-3) and GIRK(1-4) inwardly rectifying K+ channel mRNAs are differentially expressed in the adult rat brain."
      Karschin C., Dissman E., Stuehmer W., Karschin A.
      J. Neurosci. 16:3559-3570(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiKCNJ3_RAT
    AccessioniPrimary (citable) accession number: P63251
    Secondary accession number(s): P35562
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3