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P63251 (IRK3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
G protein-activated inward rectifier potassium channel 1
Short name=GIRK-1
Alternative name(s):
Inward rectifier K(+) channel Kir3.1
KGA
KGB1
Potassium channel, inwardly rectifying subfamily J member 3
Gene names
Name:Kcnj3
Synonyms:Girk1, Kga
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

Subunit structure

Associates with GIRK2, GIRK3 or GIRK4 to form a G-protein activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501G protein-activated inward rectifier potassium channel 1
PRO_0000154940

Regions

Topological domain1 – 8080Cytoplasmic
Transmembrane81 – 10525Helical; Name=M1; By similarity
Topological domain106 – 12924Extracellular By similarity
Intramembrane130 – 14112Helical; Pore-forming; Name=H5; By similarity
Intramembrane142 – 1487Pore-forming; By similarity
Topological domain149 – 1579Extracellular By similarity
Transmembrane158 – 17922Helical; Name=M2; By similarity
Topological domain180 – 501322Cytoplasmic
Motif143 – 1486Selectivity filter By similarity

Sites

Site1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P63251 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: AB8910E9CC08FFEC

FASTA50156,573
        10         20         30         40         50         60 
MSALRRKFGD DYQVVTTSSS GSGLQPQGPG QGPQQQLVPK KKRQRFVDKN GRCNVQHGNL 

        70         80         90        100        110        120 
GSETSRYLSD LFTTLVDLKW RWNLFIFILT YTVAWLFMAS MWWVIAYTRG DLNKAHVGNY 

       130        140        150        160        170        180 
TPCVANVYNF PSAFLFFIET EATIGYGYRY ITDKCPEGII LFLFQSILGS IVDAFLIGCM 

       190        200        210        220        230        240 
FIKMSQPKKR AETLMFSEHA VISMRDGKLT LMFRVGNLRN SHMVSAQIRC KLLKSRQTPE 

       250        260        270        280        290        300 
GEFLPLDQLE LDVGFSTGAD QLFLVSPLTI CHVIDAKSPF YDLSQRSMQT EQFEVVVILE 

       310        320        330        340        350        360 
GIVETTGMTC QARTSYTEDE VLWGHRFFPV ISLEEGFFKV DYSQFHATFE VPTPPYSVKE 

       370        380        390        400        410        420 
QEEMLLMSSP LIAPAITNSK ERHNSVECLD GLDDISTKLP SKLQKITGRE DFPKKLLRMS 

       430        440        450        460        470        480 
STTSEKAYSL GDLPMKLQRI SSVPGNSEEK LVSKTTKMLS DPMSQSVADL PPKLQKMAGG 

       490        500 
PTRMEGNLPA KLRKMNSDRF T

« Hide

References

[1]"Atrial G protein-activated K+ channel: expression cloning and molecular properties."
Dascal N., Schreibmayer W., Lim N.F., Wang W., Chavkin C., Dimagno L., Labarca C., Kieffer B.L., Gaveriaux-Ruff C., Trollinger D., Lester H.A., Davidson N.
Proc. Natl. Acad. Sci. U.S.A. 90:10235-10239(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain and Heart atrium.
[2]"Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel."
Kubo Y., Reuveny E., Slesinger P.A., Jan Y.N., Jan L.Y.
Nature 364:802-806(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"G protein-activated inwardly rectifying potassium channel (GIRK1/KGA) mRNA in adult rat heart and brain by in situ hybridization histochemistry."
Depaoli A.M., Bell G.I., Stoffel M.
Mol. Cell. Neurosci. 5:515-522(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"IRK(1-3) and GIRK(1-4) inwardly rectifying K+ channel mRNAs are differentially expressed in the adult rat brain."
Karschin C., Dissman E., Stuehmer W., Karschin A.
J. Neurosci. 16:3559-3570(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L25264 mRNA. Translation: AAA41226.1.
U01071 mRNA. Translation: AAC52127.1.
U01141 mRNA. Translation: AAC52125.1.
Y12259 mRNA. Translation: CAA72936.1.
U09243 mRNA. Translation: AAA18031.1.
IPIIPI00199633.
PIRI61084.
RefSeqNP_113798.1. NM_031610.3.
UniGeneRn.9809.

3D structure databases

ProteinModelPortalP63251.
SMRP63251. Positions 41-370.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-7260243.

Proteomic databases

PRIDEP63251.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000007335; ENSRNOP00000007335; ENSRNOG00000005369.
GeneID50599.
KEGGrno:50599.
UCSCRGD:2958. rat.

Organism-specific databases

CTD3760.
RGD2958. Kcnj3.

Phylogenomic databases

eggNOGNOG280776.
GeneTreeENSGT00690000101708.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP63251.
KOK04997.
OMASMQSEQF.
OrthoDBEOG4GXFMM.

Gene expression databases

GenevestigatorP63251.
GermOnlineENSRNOG00000005369. Rattus norvegicus.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003274. K_chnl_inward-rec_Kir3.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01327. KIR31CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. Ig_E-set. 1 hit.
ProtoNetSearch...

Other

BindingDBP63251.
NextBio289889.

Entry information

Entry nameIRK3_RAT
AccessionPrimary (citable) accession number: P63251
Secondary accession number(s): P35562
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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