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P63251

- KCNJ3_RAT

UniProt

P63251 - KCNJ3_RAT

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Protein

G protein-activated inward rectifier potassium channel 1

Gene

Kcnj3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei173 – 1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. G-protein activated inward rectifier potassium channel activity Source: InterPro

GO - Biological processi

  1. response to electrical stimulus Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_242287. Activation of G protein gated Potassium channels.
REACT_249237. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 1
Short name:
GIRK-1
Alternative name(s):
Inward rectifier K(+) channel Kir3.1
KGA
KGB1
Potassium channel, inwardly rectifying subfamily J member 3
Gene namesi
Name:Kcnj3
Synonyms:Girk1, Kga
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi2958. Kcnj3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8080CytoplasmicAdd
BLAST
Transmembranei81 – 10525Helical; Name=M1By similarityAdd
BLAST
Topological domaini106 – 12924ExtracellularBy similarityAdd
BLAST
Intramembranei130 – 14112Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei142 – 1487Pore-formingBy similarity
Topological domaini149 – 1579ExtracellularBy similarity
Transmembranei158 – 17922Helical; Name=M2By similarityAdd
BLAST
Topological domaini180 – 501322CytoplasmicAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: RGD
  2. external side of plasma membrane Source: RGD
  3. integral component of membrane Source: UniProtKB-KW
  4. T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501G protein-activated inward rectifier potassium channel 1PRO_0000154940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP63251.

Expressioni

Gene expression databases

GenevestigatoriP63251.

Interactioni

Subunit structurei

Associates with GIRK2, GIRK3 or GIRK4 to form a G-protein activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKACAP005176EBI-7250981,EBI-7251007From a different organism.

Protein-protein interaction databases

IntActiP63251. 1 interaction.
MINTiMINT-7260243.

Structurei

3D structure databases

ProteinModelPortaliP63251.
SMRiP63251. Positions 41-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi143 – 1486Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG280776.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP63251.
KOiK04997.
OMAiSMQSEQF.
OrthoDBiEOG7XPZ5K.
PhylomeDBiP63251.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003274. K_chnl_inward-rec_Kir3.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01327. KIR31CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P63251-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSALRRKFGD DYQVVTTSSS GSGLQPQGPG QGPQQQLVPK KKRQRFVDKN
60 70 80 90 100
GRCNVQHGNL GSETSRYLSD LFTTLVDLKW RWNLFIFILT YTVAWLFMAS
110 120 130 140 150
MWWVIAYTRG DLNKAHVGNY TPCVANVYNF PSAFLFFIET EATIGYGYRY
160 170 180 190 200
ITDKCPEGII LFLFQSILGS IVDAFLIGCM FIKMSQPKKR AETLMFSEHA
210 220 230 240 250
VISMRDGKLT LMFRVGNLRN SHMVSAQIRC KLLKSRQTPE GEFLPLDQLE
260 270 280 290 300
LDVGFSTGAD QLFLVSPLTI CHVIDAKSPF YDLSQRSMQT EQFEVVVILE
310 320 330 340 350
GIVETTGMTC QARTSYTEDE VLWGHRFFPV ISLEEGFFKV DYSQFHATFE
360 370 380 390 400
VPTPPYSVKE QEEMLLMSSP LIAPAITNSK ERHNSVECLD GLDDISTKLP
410 420 430 440 450
SKLQKITGRE DFPKKLLRMS STTSEKAYSL GDLPMKLQRI SSVPGNSEEK
460 470 480 490 500
LVSKTTKMLS DPMSQSVADL PPKLQKMAGG PTRMEGNLPA KLRKMNSDRF

T
Length:501
Mass (Da):56,573
Last modified:October 11, 2004 - v1
Checksum:iAB8910E9CC08FFEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25264 mRNA. Translation: AAA41226.1.
U01071 mRNA. Translation: AAC52127.1.
U01141 mRNA. Translation: AAC52125.1.
Y12259 mRNA. Translation: CAA72936.1.
U09243 mRNA. Translation: AAA18031.1.
PIRiI61084.
RefSeqiNP_113798.1. NM_031610.3.
UniGeneiRn.9809.

Genome annotation databases

EnsembliENSRNOT00000007335; ENSRNOP00000007335; ENSRNOG00000005369.
GeneIDi50599.
KEGGirno:50599.
UCSCiRGD:2958. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25264 mRNA. Translation: AAA41226.1 .
U01071 mRNA. Translation: AAC52127.1 .
U01141 mRNA. Translation: AAC52125.1 .
Y12259 mRNA. Translation: CAA72936.1 .
U09243 mRNA. Translation: AAA18031.1 .
PIRi I61084.
RefSeqi NP_113798.1. NM_031610.3.
UniGenei Rn.9809.

3D structure databases

ProteinModelPortali P63251.
SMRi P63251. Positions 41-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P63251. 1 interaction.
MINTi MINT-7260243.

Chemistry

BindingDBi P63251.
GuidetoPHARMACOLOGYi 434.

Proteomic databases

PRIDEi P63251.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000007335 ; ENSRNOP00000007335 ; ENSRNOG00000005369 .
GeneIDi 50599.
KEGGi rno:50599.
UCSCi RGD:2958. rat.

Organism-specific databases

CTDi 3760.
RGDi 2958. Kcnj3.

Phylogenomic databases

eggNOGi NOG280776.
GeneTreei ENSGT00760000118842.
HOGENOMi HOG000237325.
HOVERGENi HBG006178.
InParanoidi P63251.
KOi K04997.
OMAi SMQSEQF.
OrthoDBi EOG7XPZ5K.
PhylomeDBi P63251.
TreeFami TF313676.

Enzyme and pathway databases

Reactomei REACT_242287. Activation of G protein gated Potassium channels.
REACT_249237. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

NextBioi 289889.
PROi P63251.

Gene expression databases

Genevestigatori P63251.

Family and domain databases

Gene3Di 2.60.40.1400. 1 hit.
InterProi IPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003274. K_chnl_inward-rec_Kir3.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view ]
PANTHERi PTHR11767. PTHR11767. 1 hit.
Pfami PF01007. IRK. 1 hit.
[Graphical view ]
PIRSFi PIRSF005465. GIRK_kir. 1 hit.
PRINTSi PR01327. KIR31CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMi SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain and Heart atrium.
  2. "Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel."
    Kubo Y., Reuveny E., Slesinger P.A., Jan Y.N., Jan L.Y.
    Nature 364:802-806(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "G protein-activated inwardly rectifying potassium channel (GIRK1/KGA) mRNA in adult rat heart and brain by in situ hybridization histochemistry."
    Depaoli A.M., Bell G.I., Stoffel M.
    Mol. Cell. Neurosci. 5:515-522(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "IRK(1-3) and GIRK(1-4) inwardly rectifying K+ channel mRNAs are differentially expressed in the adult rat brain."
    Karschin C., Dissman E., Stuehmer W., Karschin A.
    J. Neurosci. 16:3559-3570(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiKCNJ3_RAT
AccessioniPrimary (citable) accession number: P63251
Secondary accession number(s): P35562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 26, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3