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P63250

- KCNJ3_MOUSE

UniProt

P63250 - KCNJ3_MOUSE

Protein

G protein-activated inward rectifier potassium channel 1

Gene

Kcnj3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei173 – 1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. G-protein activated inward rectifier potassium channel activity Source: MGI

    GO - Biological processi

    1. potassium ion transmembrane transport Source: GOC
    2. response to electrical stimulus Source: Ensembl

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-activated inward rectifier potassium channel 1
    Short name:
    GIRK-1
    Alternative name(s):
    Inward rectifier K(+) channel Kir3.1
    Potassium channel, inwardly rectifying subfamily J member 3
    Gene namesi
    Name:Kcnj3
    Synonyms:Girk1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:104742. Kcnj3.

    Subcellular locationi

    GO - Cellular componenti

    1. external side of plasma membrane Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. T-tubule Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501G protein-activated inward rectifier potassium channel 1PRO_0000154939Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP63250.
    PRIDEiP63250.

    Expressioni

    Gene expression databases

    ArrayExpressiP63250.
    BgeeiP63250.
    GenevestigatoriP63250.

    Interactioni

    Subunit structurei

    Associates with GIRK2, GIRK3 or GIRK4 to form a G-protein activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-48585N.
    STRINGi10090.ENSMUSP00000063329.

    Structurei

    Secondary structure

    1
    501
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi55 – 584
    Helixi69 – 779
    Helixi178 – 1858
    Helixi190 – 1923
    Beta strandi194 – 1963
    Beta strandi200 – 2056
    Beta strandi208 – 2158
    Beta strandi227 – 23711
    Beta strandi243 – 2519
    Turni255 – 2573
    Beta strandi268 – 2736
    Turni279 – 2824
    Helixi285 – 2884
    Beta strandi294 – 3018
    Turni304 – 3074
    Beta strandi310 – 3178
    Helixi318 – 3203
    Beta strandi321 – 3233
    Beta strandi325 – 3273
    Beta strandi331 – 3333
    Beta strandi335 – 3406
    Beta strandi344 – 3463
    Beta strandi348 – 3503
    Helixi358 – 36710

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1N9PX-ray1.80A41-371[»]
    1U4EX-ray2.09A41-63[»]
    2QKSX-ray2.20A/B41-82[»]
    A/B178-371[»]
    3K6NX-ray2.00A41-371[»]
    ProteinModelPortaliP63250.
    SMRiP63250. Positions 41-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63250.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8080CytoplasmicAdd
    BLAST
    Topological domaini106 – 12924ExtracellularBy similarityAdd
    BLAST
    Topological domaini149 – 1579ExtracellularBy similarity
    Topological domaini180 – 501322CytoplasmicAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei130 – 14112Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei142 – 1487Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei81 – 10525Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei158 – 17922Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi143 – 1486Selectivity filterBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG280776.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiP63250.
    KOiK04997.
    OMAiSMQSEQF.
    OrthoDBiEOG7XPZ5K.
    PhylomeDBiP63250.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003274. K_chnl_inward-rec_Kir3.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01327. KIR31CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P63250-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALRRKFGD DYQVVTTSSS GSGLQPQGPG QGPQQQLVPK KKRQRFVDKN    50
    GRCNVQHGNL GSETSRYLSD LFTTLVDLKW RWNLFIFILT YTVAWLFMAS 100
    MWWVIAYTRG DLNKAHVGNY TPCVANVYNF PSAFLFFIET EATIGYGYRY 150
    ITDKCPEGII LFLFQSILGS IVDAFLIGCM FIKMSQPKKR AETLMFSEHA 200
    VISMRDGKLT LMFRVGNLRN SHMVSAQIRC KLLKSRQTPE GEFLPLDQLE 250
    LDVGFSTGAD QLFLVSPLTI CHVIDAKSPF YDLSQRSMQT EQFEVVVILE 300
    GIVETTGMTC QARTSYTEDE VLWGHRFFPV ISLEEGFFKV DYSQFHATFE 350
    VPTPPYSVKE QEEMLLMSSP LIAPAITNSK ERHNSVECLD GLDDISTKLP 400
    SKLQKITGRE DFPKKLLRMS STTSEKAYSL GDLPMKLQRI SSVPGNSEEK 450
    LVSKTTKMLS DPMSQSVADL PPKLQKMAGG PTRMEGNLPA KLRKMNSDRF 500
    T 501
    Length:501
    Mass (Da):56,573
    Last modified:October 11, 2004 - v1
    Checksum:iAB8910E9CC08FFEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45022 mRNA. Translation: BAA08079.1.
    CCDSiCCDS16042.1.
    PIRiJC4139.
    RefSeqiNP_032452.1. NM_008426.1.
    XP_006497791.1. XM_006497728.1.
    XP_006497792.1. XM_006497729.1.
    XP_006497793.1. XM_006497730.1.
    UniGeneiMm.5127.

    Genome annotation databases

    EnsembliENSMUST00000067101; ENSMUSP00000063329; ENSMUSG00000026824.
    ENSMUST00000112633; ENSMUSP00000108252; ENSMUSG00000026824.
    GeneIDi16519.
    KEGGimmu:16519.
    UCSCiuc008jru.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45022 mRNA. Translation: BAA08079.1 .
    CCDSi CCDS16042.1.
    PIRi JC4139.
    RefSeqi NP_032452.1. NM_008426.1.
    XP_006497791.1. XM_006497728.1.
    XP_006497792.1. XM_006497729.1.
    XP_006497793.1. XM_006497730.1.
    UniGenei Mm.5127.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1N9P X-ray 1.80 A 41-371 [» ]
    1U4E X-ray 2.09 A 41-63 [» ]
    2QKS X-ray 2.20 A/B 41-82 [» ]
    A/B 178-371 [» ]
    3K6N X-ray 2.00 A 41-371 [» ]
    ProteinModelPortali P63250.
    SMRi P63250. Positions 41-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48585N.
    STRINGi 10090.ENSMUSP00000063329.

    Chemistry

    BindingDBi P63250.
    GuidetoPHARMACOLOGYi 434.

    Proteomic databases

    PaxDbi P63250.
    PRIDEi P63250.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067101 ; ENSMUSP00000063329 ; ENSMUSG00000026824 .
    ENSMUST00000112633 ; ENSMUSP00000108252 ; ENSMUSG00000026824 .
    GeneIDi 16519.
    KEGGi mmu:16519.
    UCSCi uc008jru.1. mouse.

    Organism-specific databases

    CTDi 3760.
    MGIi MGI:104742. Kcnj3.

    Phylogenomic databases

    eggNOGi NOG280776.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi P63250.
    KOi K04997.
    OMAi SMQSEQF.
    OrthoDBi EOG7XPZ5K.
    PhylomeDBi P63250.
    TreeFami TF313676.

    Enzyme and pathway databases

    Reactomei REACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Miscellaneous databases

    EvolutionaryTracei P63250.
    NextBioi 289889.
    PROi P63250.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P63250.
    Bgeei P63250.
    Genevestigatori P63250.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003274. K_chnl_inward-rec_Kir3.1.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01327. KIR31CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a mouse G-protein-activated K+ channel (mGIRK1) and distinct distributions of three GIRK (GIRK1, 2 and 3) mRNAs in mouse brain."
      Kobayashi T., Ikeda K., Ichikawa T., Abe S., Togashi S., Kumanishi T.
      Biochem. Biophys. Res. Commun. 208:1166-1173(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "Structural basis of inward rectification: cytoplasmic pore of the G protein-gated inward rectifier GIRK1 at 1.8 A resolution."
      Nishida M., MacKinnon R.
      Cell 111:957-965(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 43-63 AND 190-370.

    Entry informationi

    Entry nameiKCNJ3_MOUSE
    AccessioniPrimary (citable) accession number: P63250
    Secondary accession number(s): P35562
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3