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P63250

- KCNJ3_MOUSE

UniProt

P63250 - KCNJ3_MOUSE

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Protein
G protein-activated inward rectifier potassium channel 1
Gene
Kcnj3, Girk1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei173 – 1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

GO - Molecular functioni

  1. G-protein activated inward rectifier potassium channel activity Source: MGI

GO - Biological processi

  1. potassium ion transmembrane transport Source: GOC
  2. response to electrical stimulus Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_208837. Activation of G protein gated Potassium channels.
REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 1
Short name:
GIRK-1
Alternative name(s):
Inward rectifier K(+) channel Kir3.1
Potassium channel, inwardly rectifying subfamily J member 3
Gene namesi
Name:Kcnj3
Synonyms:Girk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:104742. Kcnj3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8080Cytoplasmic
Add
BLAST
Transmembranei81 – 10525Helical; Name=M1; By similarity
Add
BLAST
Topological domaini106 – 12924Extracellular By similarity
Add
BLAST
Intramembranei130 – 14112Helical; Pore-forming; Name=H5; By similarity
Add
BLAST
Intramembranei142 – 1487Pore-forming; By similarity
Topological domaini149 – 1579Extracellular By similarity
Transmembranei158 – 17922Helical; Name=M2; By similarity
Add
BLAST
Topological domaini180 – 501322Cytoplasmic
Add
BLAST

GO - Cellular componenti

  1. T-tubule Source: Ensembl
  2. external side of plasma membrane Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501G protein-activated inward rectifier potassium channel 1
PRO_0000154939Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi119 – 1191N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP63250.
PRIDEiP63250.

Expressioni

Gene expression databases

ArrayExpressiP63250.
BgeeiP63250.
GenevestigatoriP63250.

Interactioni

Subunit structurei

Associates with GIRK2, GIRK3 or GIRK4 to form a G-protein activated heteromultimer pore-forming unit. The resulting inward current is much larger By similarity.

Protein-protein interaction databases

DIPiDIP-48585N.
STRINGi10090.ENSMUSP00000063329.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi55 – 584
Helixi69 – 779
Helixi178 – 1858
Helixi190 – 1923
Beta strandi194 – 1963
Beta strandi200 – 2056
Beta strandi208 – 2158
Beta strandi227 – 23711
Beta strandi243 – 2519
Turni255 – 2573
Beta strandi268 – 2736
Turni279 – 2824
Helixi285 – 2884
Beta strandi294 – 3018
Turni304 – 3074
Beta strandi310 – 3178
Helixi318 – 3203
Beta strandi321 – 3233
Beta strandi325 – 3273
Beta strandi331 – 3333
Beta strandi335 – 3406
Beta strandi344 – 3463
Beta strandi348 – 3503
Helixi358 – 36710

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N9PX-ray1.80A41-371[»]
1U4EX-ray2.09A41-63[»]
2QKSX-ray2.20A/B41-82[»]
A/B178-371[»]
3K6NX-ray2.00A41-371[»]
ProteinModelPortaliP63250.
SMRiP63250. Positions 41-370.

Miscellaneous databases

EvolutionaryTraceiP63250.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi143 – 1486Selectivity filter By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG280776.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP63250.
KOiK04997.
OMAiSMQSEQF.
OrthoDBiEOG7XPZ5K.
PhylomeDBiP63250.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003274. K_chnl_inward-rec_Kir3.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01327. KIR31CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P63250-1 [UniParc]FASTAAdd to Basket

« Hide

MSALRRKFGD DYQVVTTSSS GSGLQPQGPG QGPQQQLVPK KKRQRFVDKN    50
GRCNVQHGNL GSETSRYLSD LFTTLVDLKW RWNLFIFILT YTVAWLFMAS 100
MWWVIAYTRG DLNKAHVGNY TPCVANVYNF PSAFLFFIET EATIGYGYRY 150
ITDKCPEGII LFLFQSILGS IVDAFLIGCM FIKMSQPKKR AETLMFSEHA 200
VISMRDGKLT LMFRVGNLRN SHMVSAQIRC KLLKSRQTPE GEFLPLDQLE 250
LDVGFSTGAD QLFLVSPLTI CHVIDAKSPF YDLSQRSMQT EQFEVVVILE 300
GIVETTGMTC QARTSYTEDE VLWGHRFFPV ISLEEGFFKV DYSQFHATFE 350
VPTPPYSVKE QEEMLLMSSP LIAPAITNSK ERHNSVECLD GLDDISTKLP 400
SKLQKITGRE DFPKKLLRMS STTSEKAYSL GDLPMKLQRI SSVPGNSEEK 450
LVSKTTKMLS DPMSQSVADL PPKLQKMAGG PTRMEGNLPA KLRKMNSDRF 500
T 501
Length:501
Mass (Da):56,573
Last modified:October 11, 2004 - v1
Checksum:iAB8910E9CC08FFEC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D45022 mRNA. Translation: BAA08079.1.
CCDSiCCDS16042.1.
PIRiJC4139.
RefSeqiNP_032452.1. NM_008426.1.
XP_006497791.1. XM_006497728.1.
XP_006497792.1. XM_006497729.1.
XP_006497793.1. XM_006497730.1.
UniGeneiMm.5127.

Genome annotation databases

EnsembliENSMUST00000067101; ENSMUSP00000063329; ENSMUSG00000026824.
ENSMUST00000112633; ENSMUSP00000108252; ENSMUSG00000026824.
GeneIDi16519.
KEGGimmu:16519.
UCSCiuc008jru.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D45022 mRNA. Translation: BAA08079.1 .
CCDSi CCDS16042.1.
PIRi JC4139.
RefSeqi NP_032452.1. NM_008426.1.
XP_006497791.1. XM_006497728.1.
XP_006497792.1. XM_006497729.1.
XP_006497793.1. XM_006497730.1.
UniGenei Mm.5127.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1N9P X-ray 1.80 A 41-371 [» ]
1U4E X-ray 2.09 A 41-63 [» ]
2QKS X-ray 2.20 A/B 41-82 [» ]
A/B 178-371 [» ]
3K6N X-ray 2.00 A 41-371 [» ]
ProteinModelPortali P63250.
SMRi P63250. Positions 41-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48585N.
STRINGi 10090.ENSMUSP00000063329.

Chemistry

BindingDBi P63250.
GuidetoPHARMACOLOGYi 434.

Proteomic databases

PaxDbi P63250.
PRIDEi P63250.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000067101 ; ENSMUSP00000063329 ; ENSMUSG00000026824 .
ENSMUST00000112633 ; ENSMUSP00000108252 ; ENSMUSG00000026824 .
GeneIDi 16519.
KEGGi mmu:16519.
UCSCi uc008jru.1. mouse.

Organism-specific databases

CTDi 3760.
MGIi MGI:104742. Kcnj3.

Phylogenomic databases

eggNOGi NOG280776.
HOGENOMi HOG000237325.
HOVERGENi HBG006178.
InParanoidi P63250.
KOi K04997.
OMAi SMQSEQF.
OrthoDBi EOG7XPZ5K.
PhylomeDBi P63250.
TreeFami TF313676.

Enzyme and pathway databases

Reactomei REACT_208837. Activation of G protein gated Potassium channels.
REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

EvolutionaryTracei P63250.
NextBioi 289889.
PROi P63250.
SOURCEi Search...

Gene expression databases

ArrayExpressi P63250.
Bgeei P63250.
Genevestigatori P63250.

Family and domain databases

Gene3Di 2.60.40.1400. 1 hit.
InterProi IPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003274. K_chnl_inward-rec_Kir3.1.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view ]
PANTHERi PTHR11767. PTHR11767. 1 hit.
Pfami PF01007. IRK. 1 hit.
[Graphical view ]
PIRSFi PIRSF005465. GIRK_kir. 1 hit.
PRINTSi PR01327. KIR31CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMi SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a mouse G-protein-activated K+ channel (mGIRK1) and distinct distributions of three GIRK (GIRK1, 2 and 3) mRNAs in mouse brain."
    Kobayashi T., Ikeda K., Ichikawa T., Abe S., Togashi S., Kumanishi T.
    Biochem. Biophys. Res. Commun. 208:1166-1173(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Structural basis of inward rectification: cytoplasmic pore of the G protein-gated inward rectifier GIRK1 at 1.8 A resolution."
    Nishida M., MacKinnon R.
    Cell 111:957-965(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 43-63 AND 190-370.

Entry informationi

Entry nameiKCNJ3_MOUSE
AccessioniPrimary (citable) accession number: P63250
Secondary accession number(s): P35562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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