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Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

Pkia

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 161Important for inhibitionBy similarity
Sitei19 – 191Important for inhibitionBy similarity
Sitei20 – 201Important for inhibitionBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein kinase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene namesi
Name:Pkia
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621021. Pkia.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 7675cAMP-dependent protein kinase inhibitor alphaPRO_0000154536Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP63249.

Expressioni

Tissue specificityi

Highest expression in muscle (both skeletal and cardiac) and brain.

Gene expression databases

GenevisibleiP63249. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016567.

Structurei

Secondary structure

1
76
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 126Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L3RX-ray2.00I6-25[»]
1Q62X-ray2.30I6-25[»]
1Q8UX-ray1.90B6-25[»]
1SVHX-ray2.30B6-25[»]
1YDSX-ray2.20I6-25[»]
1YDTX-ray2.30I6-25[»]
ProteinModelPortaliP63249.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63249.

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiENOG410J11W. Eukaryota.
ENOG410YT4B. LUCA.
GeneTreeiENSGT00530000064276.
HOGENOMiHOG000059641.
HOVERGENiHBG101093.
InParanoidiP63249.
KOiK15985.
OMAiTTNTTDV.
OrthoDBiEOG7JHM8S.
PhylomeDBiP63249.
TreeFamiTF330809.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEDDGQRSS TEQSGEAQGE AAKSES
Length:76
Mass (Da):7,960
Last modified:January 23, 2007 - v2
Checksum:iFCCE07281498788A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02615 mRNA. Translation: AAA40867.1.
RefSeqiNP_446224.1. NM_053772.2.
XP_006232217.1. XM_006232155.2.
XP_006232218.1. XM_006232156.2.
UniGeneiRn.94808.

Genome annotation databases

EnsembliENSRNOT00000016567; ENSRNOP00000016567; ENSRNOG00000012095.
GeneIDi114906.
KEGGirno:114906.
UCSCiRGD:621021. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02615 mRNA. Translation: AAA40867.1.
RefSeqiNP_446224.1. NM_053772.2.
XP_006232217.1. XM_006232155.2.
XP_006232218.1. XM_006232156.2.
UniGeneiRn.94808.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L3RX-ray2.00I6-25[»]
1Q62X-ray2.30I6-25[»]
1Q8UX-ray1.90B6-25[»]
1SVHX-ray2.30B6-25[»]
1YDSX-ray2.20I6-25[»]
1YDTX-ray2.30I6-25[»]
ProteinModelPortaliP63249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016567.

Proteomic databases

PaxDbiP63249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016567; ENSRNOP00000016567; ENSRNOG00000012095.
GeneIDi114906.
KEGGirno:114906.
UCSCiRGD:621021. rat.

Organism-specific databases

CTDi5569.
RGDi621021. Pkia.

Phylogenomic databases

eggNOGiENOG410J11W. Eukaryota.
ENOG410YT4B. LUCA.
GeneTreeiENSGT00530000064276.
HOGENOMiHOG000059641.
HOVERGENiHBG101093.
InParanoidiP63249.
KOiK15985.
OMAiTTNTTDV.
OrthoDBiEOG7JHM8S.
PhylomeDBiP63249.
TreeFamiTF330809.

Miscellaneous databases

EvolutionaryTraceiP63249.
NextBioi294971.
PROiP63249.

Gene expression databases

GenevisibleiP63249. RN.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. "The alpha- and beta-isoforms of the inhibitor protein of the 3',5'-cyclic adenosine monophosphate-dependent protein kinase: characteristics and tissue- and developmental-specific expression."
    van Patten S.M., Howard P., Walsh D.A., Maurer R.A.
    Mol. Endocrinol. 6:2114-2122(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiIPKA_RAT
AccessioniPrimary (citable) accession number: P63249
Secondary accession number(s): P27776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.