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Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

Pkia

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Important for inhibitionBy similarity1
Sitei19Important for inhibitionBy similarity1
Sitei20Important for inhibitionBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtein kinase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene namesi
Name:Pkia
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi621021 Pkia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001545362 – 76cAMP-dependent protein kinase inhibitor alphaAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP63249
PRIDEiP63249

Expressioni

Tissue specificityi

Highest expression in muscle (both skeletal and cardiac) and brain.

Gene expression databases

BgeeiENSRNOG00000012095
GenevisibleiP63249 RN

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016567

Structurei

Secondary structure

176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L3RX-ray2.00I6-25[»]
1Q62X-ray2.30I6-25[»]
1Q8UX-ray1.90B6-25[»]
1SVHX-ray2.30B6-25[»]
1YDSX-ray2.20I6-25[»]
1YDTX-ray2.30I6-25[»]
ProteinModelPortaliP63249
SMRiP63249
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63249

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiENOG410J11W Eukaryota
ENOG410YT4B LUCA
GeneTreeiENSGT00530000064276
HOGENOMiHOG000059641
HOVERGENiHBG101093
InParanoidiP63249
KOiK15985
OMAiCGYLLAM
OrthoDBiEOG091G0LVC
PhylomeDBiP63249
TreeFamiTF330809

Family and domain databases

InterProiView protein in InterPro
IPR004171 cAMP_dep_PKI
PANTHERiPTHR15416 PTHR15416, 1 hit
PfamiView protein in Pfam
PF02827 PKI, 1 hit
PIRSFiPIRSF001667 PKI, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD010366 cAMP_dep_PKI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEDDGQRSS TEQSGEAQGE AAKSES
Length:76
Mass (Da):7,960
Last modified:January 23, 2007 - v2
Checksum:iFCCE07281498788A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02615 mRNA Translation: AAA40867.1
RefSeqiNP_446224.1, NM_053772.2
XP_006232217.1, XM_006232155.3
XP_006232218.1, XM_006232156.3
UniGeneiRn.94808

Genome annotation databases

EnsembliENSRNOT00000016567; ENSRNOP00000016567; ENSRNOG00000012095
GeneIDi114906
KEGGirno:114906
UCSCiRGD:621021 rat

Similar proteinsi

Entry informationi

Entry nameiIPKA_RAT
AccessioniPrimary (citable) accession number: P63249
Secondary accession number(s): P27776
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 84 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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