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Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

Pkia

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Important for inhibitionBy similarity1
Sitei19Important for inhibitionBy similarity1
Sitei20Important for inhibitionBy similarity1

GO - Molecular functioni

  • cAMP-dependent protein kinase inhibitor activity Source: MGI
  • protein kinase A catalytic subunit binding Source: CAFA

GO - Biological processi

  • negative regulation of cAMP-dependent protein kinase activity Source: MGI
  • negative regulation of catalytic activity Source: MGI
  • negative regulation of protein import into nucleus Source: MGI
  • negative regulation of protein kinase activity Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • regulation of G2/M transition of mitotic cell cycle Source: MGI

Keywordsi

Molecular functionProtein kinase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene namesi
Name:Pkia
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:104747 Pkia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001545332 – 76cAMP-dependent protein kinase inhibitor alphaAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP63248
PeptideAtlasiP63248
PRIDEiP63248

PTM databases

PhosphoSitePlusiP63248

Expressioni

Tissue specificityi

Present at high levels in skeletal muscle and brain but is present at lower levels in heart, testis and liver.

Gene expression databases

BgeeiENSMUSG00000027499
CleanExiMM_PKIA
ExpressionAtlasiP63248 baseline and differential
GenevisibleiP63248 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PrkacaP051322EBI-2931786,EBI-400564

GO - Molecular functioni

  • protein kinase A catalytic subunit binding Source: CAFA

Protein-protein interaction databases

BioGridi202207, 1 interactor
DIPiDIP-6087N
IntActiP63248, 3 interactors
MINTiP63248
STRINGi10090.ENSMUSP00000028999

Structurei

Secondary structure

176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1APMX-ray2.00I6-25[»]
1ATPX-ray2.20I6-25[»]
1PVKmodel-I6-25[»]
2CPKX-ray2.70I6-25[»]
2GNFX-ray2.28I6-25[»]
2GNGX-ray1.87I6-25[»]
2QURX-ray2.50B6-25[»]
3FJQX-ray1.60I6-25[»]
3OW3X-ray1.90B6-25[»]
3QALX-ray1.70I6-23[»]
3QAMX-ray1.92I6-24[»]
4DFXX-ray1.35I6-25[»]
4DFZX-ray2.00I6-25[»]
4DG0X-ray2.00I6-25[»]
4DG2X-ray2.00I6-25[»]
4DG3X-ray1.80A6-25[»]
4DH1X-ray2.00I6-25[»]
4DH3X-ray2.20I6-25[»]
4DH5X-ray2.20I6-25[»]
4DH7X-ray1.80I6-25[»]
4DH8X-ray2.30I6-25[»]
4HPTX-ray2.15I6-25[»]
4HPUX-ray1.55I6-25[»]
ProteinModelPortaliP63248
SMRiP63248
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63248

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiENOG410J11W Eukaryota
ENOG410YT4B LUCA
GeneTreeiENSGT00530000064276
HOGENOMiHOG000059641
HOVERGENiHBG101093
InParanoidiP63248
KOiK15985
OMAiCGYLLAM
OrthoDBiEOG091G0LVC
PhylomeDBiP63248
TreeFamiTF330809

Family and domain databases

InterProiView protein in InterPro
IPR004171 cAMP_dep_PKI
PANTHERiPTHR15416 PTHR15416, 1 hit
PfamiView protein in Pfam
PF02827 PKI, 1 hit
PIRSFiPIRSF001667 PKI, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD010366 cAMP_dep_PKI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEDDGQRSS TEQSGEAQGE AAKSES
Length:76
Mass (Da):7,960
Last modified:January 23, 2007 - v2
Checksum:iFCCE07281498788A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63554 mRNA Translation: AAA39940.1
AK139347 mRNA Translation: BAE23970.1
CH466577 Genomic DNA Translation: EDL05208.1
CH466577 Genomic DNA Translation: EDL05209.1
BC048244 mRNA Translation: AAH48244.1
CCDSiCCDS38383.1
PIRiA40536
RefSeqiNP_032888.1, NM_008862.3
UniGeneiMm.3193

Genome annotation databases

EnsembliENSMUST00000028999; ENSMUSP00000028999; ENSMUSG00000027499
ENSMUST00000193330; ENSMUSP00000141466; ENSMUSG00000027499
GeneIDi18767
KEGGimmu:18767
UCSCiuc008oof.1 mouse

Similar proteinsi

Entry informationi

Entry nameiIPKA_MOUSE
AccessioniPrimary (citable) accession number: P63248
Secondary accession number(s): P27776, Q3UTL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health