P63248 (IPKA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name=PKI-alpha

Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform

Gene names

Name:

Pkia

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.
Tissue specificity	Present at high levels in skeletal muscle and brain but is present at lower levels in heart, testis and liver.
Miscellaneous	The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site By similarity.
Sequence similarities	Belongs to the PKI family.

Ontologies

Keywords
Molecular function	Protein kinase inhibitor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of protein import into nucleus Inferred from direct assay. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI soluble fraction Inferred from direct assay. Source: MGI
Molecular function	cAMP-dependent protein kinase inhibitor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 76

75

cAMP-dependent protein kinase inhibitor alpha

PRO_0000154533

Sites

Site

16

1

Important for inhibition By similarity

Site

19

1

Important for inhibition By similarity

Site

20

1

Important for inhibition By similarity

Amino acid modifications

Modified residue

2

1

Blocked amino end (Thr) By similarity

Secondary structure

1

.

.

.

76

Helix Strand Turn

Details...

Helix

7 – 13

7

Sequences

	Sequence		Length	Mass (Da)
	P63248 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: FCCE07281498788A	FASTA	76	7,960
	10 20 30 40 50 60 MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT EGEDDGQRSS 70 TEQSGEAQGE AAKSES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cDNA clones for an inhibitor protein of cAMP-dependent protein kinase." Olsen S.R., Uhler M.D. J. Biol. Chem. 266:11158-11162(1991) [PubMed: 1710219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
[3]	"Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor." Zheng J., Knighton D.R., ten Eyck L.F., Karlsson R., Xuong N.-H., Taylor S.S., Sowadski J.M. Biochemistry 32:2154-2161(1993) [PubMed: 8443157] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 6-25 IN COMPLEX WITH PRKACA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M63554 mRNA. Translation: AAA39940.1.
BC048244 mRNA. Translation: AAH48244.1.

IPI

IPI00230006.

PIR

A40536.

RefSeq

NP_032888.1. NM_008862.3.

UniGene

Mm.3193.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1APM	X-ray	2.00	I	6-25	[»]
1ATP	X-ray	2.20	I	6-25	[»]
1PVK	model	-	I	6-25	[»]
2CPK	X-ray	2.70	I	6-25	[»]
2GNF	X-ray	2.28	I	6-25	[»]
2GNG	X-ray	1.87	I	6-25	[»]
2QUR	X-ray	2.50	B	6-25	[»]
3FJQ	X-ray	1.60	I	6-25	[»]
3OW3	X-ray	1.90	B	6-25	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6087N.

IntAct

P63248. 1 interaction.

STRING

P63248.

PTM databases

PhosphoSite

P63248.

Proteomic databases

PRIDE

P63248.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000028999; ENSMUSP00000028999; ENSMUSG00000027499.

GeneID

18767.

KEGG

mmu:18767.

Organism-specific databases

CTD

5569.

MGI

MGI:104747. Pkia.

Phylogenomic databases

eggNOG

roNOG17253.

HOGENOM

HBG268011.

HOVERGEN

HBG101093.

InParanoid

P63248.

OMA

NKTEGEG.

OrthoDB

EOG4C87V2.

Gene expression databases

ArrayExpress

P63248.

Bgee

P63248.

CleanEx

MM_PKIA.

Genevestigator

P63248.

GermOnline

ENSMUSG00000027499. Mus musculus.

Family and domain databases

InterPro

IPR004171. cAMP_dep_PKI.
[Graphical view]

Pfam

PF02827. PKI. 1 hit.
[Graphical view]

PIRSF

PIRSF001667. PKI. 1 hit.

ProDom

PD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

NextBio

294971.

SOURCE

Search...

Entry information

Entry name

IPKA_MOUSE

Accession

Primary (citable) accession number: P63248
Secondary accession number(s): P27776

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program