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Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

Pkia

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Important for inhibitionBy similarity1
Sitei19Important for inhibitionBy similarity1
Sitei20Important for inhibitionBy similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of cAMP-dependent protein kinase activity Source: MGI
  • negative regulation of catalytic activity Source: MGI
  • negative regulation of protein import into nucleus Source: MGI
  • negative regulation of protein kinase activity Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of G2/M transition of mitotic cell cycle Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protein kinase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene namesi
Name:Pkia
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:104747. Pkia.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001545332 – 76cAMP-dependent protein kinase inhibitor alphaAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP63248.
PeptideAtlasiP63248.
PRIDEiP63248.

PTM databases

PhosphoSitePlusiP63248.

Expressioni

Tissue specificityi

Present at high levels in skeletal muscle and brain but is present at lower levels in heart, testis and liver.

Gene expression databases

BgeeiENSMUSG00000027499.
CleanExiMM_PKIA.
ExpressionAtlasiP63248. baseline and differential.
GenevisibleiP63248. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202207. 1 interactor.
DIPiDIP-6087N.
IntActiP63248. 2 interactors.
MINTiMINT-4098688.
STRINGi10090.ENSMUSP00000028999.

Structurei

Secondary structure

176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1APMX-ray2.00I6-25[»]
1ATPX-ray2.20I6-25[»]
1PVKmodel-I6-25[»]
2CPKX-ray2.70I6-25[»]
2GNFX-ray2.28I6-25[»]
2GNGX-ray1.87I6-25[»]
2QURX-ray2.50B6-25[»]
3FJQX-ray1.60I6-25[»]
3OW3X-ray1.90B6-25[»]
3QALX-ray1.70I6-23[»]
3QAMX-ray1.92I6-24[»]
4DFXX-ray1.35I6-25[»]
4DFZX-ray2.00I6-25[»]
4DG0X-ray2.00I6-25[»]
4DG2X-ray2.00I6-25[»]
4DG3X-ray1.80A6-25[»]
4DH1X-ray2.00I6-25[»]
4DH3X-ray2.20I6-25[»]
4DH5X-ray2.20I6-25[»]
4DH7X-ray1.80I6-25[»]
4DH8X-ray2.30I6-25[»]
4HPTX-ray2.15I6-25[»]
4HPUX-ray1.55I6-25[»]
ProteinModelPortaliP63248.
SMRiP63248.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63248.

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiENOG410J11W. Eukaryota.
ENOG410YT4B. LUCA.
GeneTreeiENSGT00530000064276.
HOGENOMiHOG000059641.
HOVERGENiHBG101093.
InParanoidiP63248.
KOiK15985.
OMAiCGYLLAM.
OrthoDBiEOG091G0LVC.
PhylomeDBiP63248.
TreeFamiTF330809.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEDDGQRSS TEQSGEAQGE AAKSES
Length:76
Mass (Da):7,960
Last modified:January 23, 2007 - v2
Checksum:iFCCE07281498788A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63554 mRNA. Translation: AAA39940.1.
AK139347 mRNA. Translation: BAE23970.1.
CH466577 Genomic DNA. Translation: EDL05208.1.
CH466577 Genomic DNA. Translation: EDL05209.1.
BC048244 mRNA. Translation: AAH48244.1.
CCDSiCCDS38383.1.
PIRiA40536.
RefSeqiNP_032888.1. NM_008862.3.
UniGeneiMm.3193.

Genome annotation databases

EnsembliENSMUST00000028999; ENSMUSP00000028999; ENSMUSG00000027499.
ENSMUST00000193330; ENSMUSP00000141466; ENSMUSG00000027499.
GeneIDi18767.
KEGGimmu:18767.
UCSCiuc008oof.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63554 mRNA. Translation: AAA39940.1.
AK139347 mRNA. Translation: BAE23970.1.
CH466577 Genomic DNA. Translation: EDL05208.1.
CH466577 Genomic DNA. Translation: EDL05209.1.
BC048244 mRNA. Translation: AAH48244.1.
CCDSiCCDS38383.1.
PIRiA40536.
RefSeqiNP_032888.1. NM_008862.3.
UniGeneiMm.3193.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1APMX-ray2.00I6-25[»]
1ATPX-ray2.20I6-25[»]
1PVKmodel-I6-25[»]
2CPKX-ray2.70I6-25[»]
2GNFX-ray2.28I6-25[»]
2GNGX-ray1.87I6-25[»]
2QURX-ray2.50B6-25[»]
3FJQX-ray1.60I6-25[»]
3OW3X-ray1.90B6-25[»]
3QALX-ray1.70I6-23[»]
3QAMX-ray1.92I6-24[»]
4DFXX-ray1.35I6-25[»]
4DFZX-ray2.00I6-25[»]
4DG0X-ray2.00I6-25[»]
4DG2X-ray2.00I6-25[»]
4DG3X-ray1.80A6-25[»]
4DH1X-ray2.00I6-25[»]
4DH3X-ray2.20I6-25[»]
4DH5X-ray2.20I6-25[»]
4DH7X-ray1.80I6-25[»]
4DH8X-ray2.30I6-25[»]
4HPTX-ray2.15I6-25[»]
4HPUX-ray1.55I6-25[»]
ProteinModelPortaliP63248.
SMRiP63248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202207. 1 interactor.
DIPiDIP-6087N.
IntActiP63248. 2 interactors.
MINTiMINT-4098688.
STRINGi10090.ENSMUSP00000028999.

PTM databases

PhosphoSitePlusiP63248.

Proteomic databases

PaxDbiP63248.
PeptideAtlasiP63248.
PRIDEiP63248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028999; ENSMUSP00000028999; ENSMUSG00000027499.
ENSMUST00000193330; ENSMUSP00000141466; ENSMUSG00000027499.
GeneIDi18767.
KEGGimmu:18767.
UCSCiuc008oof.1. mouse.

Organism-specific databases

CTDi5569.
MGIiMGI:104747. Pkia.

Phylogenomic databases

eggNOGiENOG410J11W. Eukaryota.
ENOG410YT4B. LUCA.
GeneTreeiENSGT00530000064276.
HOGENOMiHOG000059641.
HOVERGENiHBG101093.
InParanoidiP63248.
KOiK15985.
OMAiCGYLLAM.
OrthoDBiEOG091G0LVC.
PhylomeDBiP63248.
TreeFamiTF330809.

Miscellaneous databases

EvolutionaryTraceiP63248.
PROiP63248.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027499.
CleanExiMM_PKIA.
ExpressionAtlasiP63248. baseline and differential.
GenevisibleiP63248. MM.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiIPKA_MOUSE
AccessioniPrimary (citable) accession number: P63248
Secondary accession number(s): P27776, Q3UTL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site (By similarity).By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.