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P63248

- IPKA_MOUSE

UniProt

P63248 - IPKA_MOUSE

Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

Pkia

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei16 – 161Important for inhibitionBy similarity
    Sitei19 – 191Important for inhibitionBy similarity
    Sitei20 – 201Important for inhibitionBy similarity

    GO - Molecular functioni

    1. cAMP-dependent protein kinase inhibitor activity Source: MGI

    GO - Biological processi

    1. negative regulation of cAMP-dependent protein kinase activity Source: MGI
    2. negative regulation of catalytic activity Source: MGI
    3. negative regulation of protein import into nucleus Source: MGI
    4. negative regulation of protein kinase activity Source: MGI
    5. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    6. regulation of G2/M transition of mitotic cell cycle Source: MGI

    Keywords - Molecular functioni

    Protein kinase inhibitor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-dependent protein kinase inhibitor alpha
    Short name:
    PKI-alpha
    Alternative name(s):
    cAMP-dependent protein kinase inhibitor, muscle/brain isoform
    Gene namesi
    Name:Pkia
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:104747. Pkia.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 7675cAMP-dependent protein kinase inhibitor alphaPRO_0000154533Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP63248.
    PaxDbiP63248.
    PRIDEiP63248.

    PTM databases

    PhosphoSiteiP63248.

    Expressioni

    Tissue specificityi

    Present at high levels in skeletal muscle and brain but is present at lower levels in heart, testis and liver.

    Gene expression databases

    BgeeiP63248.
    CleanExiMM_PKIA.
    GenevestigatoriP63248.

    Interactioni

    Protein-protein interaction databases

    BioGridi202207. 1 interaction.
    DIPiDIP-6087N.
    IntActiP63248. 2 interactions.
    MINTiMINT-4098688.
    STRINGi10090.ENSMUSP00000028999.

    Structurei

    Secondary structure

    1
    76
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 126

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1APMX-ray2.00I6-25[»]
    1ATPX-ray2.20I6-25[»]
    1PVKmodel-I6-25[»]
    2CPKX-ray2.70I6-25[»]
    2GNFX-ray2.28I6-25[»]
    2GNGX-ray1.87I6-25[»]
    2QURX-ray2.50B6-25[»]
    3FJQX-ray1.60I6-25[»]
    3OW3X-ray1.90B6-25[»]
    3QALX-ray1.70I6-23[»]
    3QAMX-ray1.92I6-24[»]
    4DFXX-ray1.35I6-25[»]
    4DFZX-ray2.00I6-25[»]
    4DG0X-ray2.00I6-25[»]
    4DG2X-ray2.00I6-25[»]
    4DG3X-ray1.80A6-25[»]
    4DH1X-ray2.00I6-25[»]
    4DH3X-ray2.20I6-25[»]
    4DH5X-ray2.20I6-25[»]
    4DH7X-ray1.80I6-25[»]
    4DH8X-ray2.30I6-25[»]
    4HPTX-ray2.15I6-25[»]
    4HPUX-ray1.55I6-25[»]
    ProteinModelPortaliP63248.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63248.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PKI family.Curated

    Phylogenomic databases

    eggNOGiNOG45574.
    GeneTreeiENSGT00530000064276.
    HOGENOMiHOG000059641.
    HOVERGENiHBG101093.
    InParanoidiP63248.
    KOiK15985.
    OMAiTTNTTDV.
    OrthoDBiEOG7JHM8S.
    PhylomeDBiP63248.
    TreeFamiTF330809.

    Family and domain databases

    InterProiIPR004171. cAMP_dep_PKI.
    [Graphical view]
    PfamiPF02827. PKI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001667. PKI. 1 hit.
    ProDomiPD010366. cAMP_dep_PKI. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P63248-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT   50
    EGEDDGQRSS TEQSGEAQGE AAKSES 76
    Length:76
    Mass (Da):7,960
    Last modified:January 23, 2007 - v2
    Checksum:iFCCE07281498788A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63554 mRNA. Translation: AAA39940.1.
    AK139347 mRNA. Translation: BAE23970.1.
    CH466577 Genomic DNA. Translation: EDL05208.1.
    CH466577 Genomic DNA. Translation: EDL05209.1.
    BC048244 mRNA. Translation: AAH48244.1.
    CCDSiCCDS38383.1.
    PIRiA40536.
    RefSeqiNP_032888.1. NM_008862.3.
    UniGeneiMm.3193.

    Genome annotation databases

    EnsembliENSMUST00000028999; ENSMUSP00000028999; ENSMUSG00000027499.
    GeneIDi18767.
    KEGGimmu:18767.
    UCSCiuc008oof.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63554 mRNA. Translation: AAA39940.1 .
    AK139347 mRNA. Translation: BAE23970.1 .
    CH466577 Genomic DNA. Translation: EDL05208.1 .
    CH466577 Genomic DNA. Translation: EDL05209.1 .
    BC048244 mRNA. Translation: AAH48244.1 .
    CCDSi CCDS38383.1.
    PIRi A40536.
    RefSeqi NP_032888.1. NM_008862.3.
    UniGenei Mm.3193.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1APM X-ray 2.00 I 6-25 [» ]
    1ATP X-ray 2.20 I 6-25 [» ]
    1PVK model - I 6-25 [» ]
    2CPK X-ray 2.70 I 6-25 [» ]
    2GNF X-ray 2.28 I 6-25 [» ]
    2GNG X-ray 1.87 I 6-25 [» ]
    2QUR X-ray 2.50 B 6-25 [» ]
    3FJQ X-ray 1.60 I 6-25 [» ]
    3OW3 X-ray 1.90 B 6-25 [» ]
    3QAL X-ray 1.70 I 6-23 [» ]
    3QAM X-ray 1.92 I 6-24 [» ]
    4DFX X-ray 1.35 I 6-25 [» ]
    4DFZ X-ray 2.00 I 6-25 [» ]
    4DG0 X-ray 2.00 I 6-25 [» ]
    4DG2 X-ray 2.00 I 6-25 [» ]
    4DG3 X-ray 1.80 A 6-25 [» ]
    4DH1 X-ray 2.00 I 6-25 [» ]
    4DH3 X-ray 2.20 I 6-25 [» ]
    4DH5 X-ray 2.20 I 6-25 [» ]
    4DH7 X-ray 1.80 I 6-25 [» ]
    4DH8 X-ray 2.30 I 6-25 [» ]
    4HPT X-ray 2.15 I 6-25 [» ]
    4HPU X-ray 1.55 I 6-25 [» ]
    ProteinModelPortali P63248.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202207. 1 interaction.
    DIPi DIP-6087N.
    IntActi P63248. 2 interactions.
    MINTi MINT-4098688.
    STRINGi 10090.ENSMUSP00000028999.

    PTM databases

    PhosphoSitei P63248.

    Proteomic databases

    MaxQBi P63248.
    PaxDbi P63248.
    PRIDEi P63248.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028999 ; ENSMUSP00000028999 ; ENSMUSG00000027499 .
    GeneIDi 18767.
    KEGGi mmu:18767.
    UCSCi uc008oof.1. mouse.

    Organism-specific databases

    CTDi 5569.
    MGIi MGI:104747. Pkia.

    Phylogenomic databases

    eggNOGi NOG45574.
    GeneTreei ENSGT00530000064276.
    HOGENOMi HOG000059641.
    HOVERGENi HBG101093.
    InParanoidi P63248.
    KOi K15985.
    OMAi TTNTTDV.
    OrthoDBi EOG7JHM8S.
    PhylomeDBi P63248.
    TreeFami TF330809.

    Miscellaneous databases

    EvolutionaryTracei P63248.
    NextBioi 294971.
    PROi P63248.
    SOURCEi Search...

    Gene expression databases

    Bgeei P63248.
    CleanExi MM_PKIA.
    Genevestigatori P63248.

    Family and domain databases

    InterProi IPR004171. cAMP_dep_PKI.
    [Graphical view ]
    Pfami PF02827. PKI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001667. PKI. 1 hit.
    ProDomi PD010366. cAMP_dep_PKI. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of cDNA clones for an inhibitor protein of cAMP-dependent protein kinase."
      Olsen S.R., Uhler M.D.
      J. Biol. Chem. 266:11158-11162(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Brain cortex.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Olfactory epithelium.
    5. "Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor."
      Zheng J., Knighton D.R., ten Eyck L.F., Karlsson R., Xuong N.-H., Taylor S.S., Sowadski J.M.
      Biochemistry 32:2154-2161(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 6-25 IN COMPLEX WITH PRKACA.

    Entry informationi

    Entry nameiIPKA_MOUSE
    AccessioniPrimary (citable) accession number: P63248
    Secondary accession number(s): P27776, Q3UTL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site By similarity.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3