Reviewed, UniProtKB/Swiss-Prot P63244 (GBLP_HUMAN)

Names and origin

Protein names

Recommended name:
    Guanine nucleotide-binding protein subunit beta-2-like 1
Alternative name(s):
    Guanine nucleotide-binding protein subunit beta-like protein 12.3
    Receptor of activated protein kinase C 1
      Short name=RACK1
    Receptor for activated C kinase
    Cell proliferation-inducing gene 21 protein
    Human lung cancer oncogene 7 protein
      Short name=HLC-7

Gene names

Name:	GNB2L1
ORF Names:	HLC7, PIG21

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation. Ref.15
Subunit structure	Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4 and KRT1. Ref.15 Ref.11 Ref.12 Ref.13
Subcellular location	Cell membrane. Note: Located on plasma membrane of neuroblastoma NMB7 cells. Ref.15
Domain	The WD repeats domain 5 mediates interaction with TRIM63.
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.
Sequence caution	The sequence AAR24619.1 differs from that shown. Reason: Frameshift at position 94.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat WD repeat
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein phosphatase binding Inferred from physical interaction. Source: UniProtKB receptor binding Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL2L11	O43521	2	EBI-296739,EBI-526406
Bcl2l11	O54918-1	2	EBI-296739,EBI-526076	From a different organism.
DYNLL1	P63167	3	EBI-296739,EBI-349105
IFNAR2	P48551	4	EBI-296739,EBI-958408
LRP12	Q9Y561	1	EBI-296739,EBI-296693

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.9
	Chain	2 – 317	316	Guanine nucleotide-binding protein subunit beta-2-like 1		PRO_0000127731
Regions
	Repeat	13 – 44	32	WD 1
	Repeat	61 – 91	31	WD 2
	Repeat	103 – 133	31	WD 3
	Repeat	146 – 178	33	WD 4
	Repeat	190 – 220	31	WD 5
	Repeat	231 – 260	30	WD 6
	Repeat	281 – 311	31	WD 7
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine Ref.9
	Modified residue	228	1	Phosphotyrosine Ref.14
Experimental info
	Sequence conflict	70 – 111	42	Missing in BAG53102. Ref.4
	Sequence conflict	94	1	T → TRK in AAR24619. Ref.3
	Sequence conflict	221	1	L → F in BAD96208. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P63244-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 257F91E369ED2044	FASTA	317	35,077
	10 20 30 40 50 60 MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR 70 80 90 100 110 120 GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI 130 140 150 160 170 180 VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA 190 200 210 220 230 240 NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC 250 260 270 280 290 300 FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA 310 GYTDNLVRVW QVTIGTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Physical linkage of a guanine nucleotide-binding protein-related gene to the chicken major histocompatibility complex." Guillemot F., Billault A., Auffray C. Proc. Natl. Acad. Sci. U.S.A. 86:4594-4598(1989) [PubMed: 2499885] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of new tumor-related gene in human lung cancer." Kim J.W. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Identification of a proliferation-inducing gene." Kim J.W. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adipose tissue.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell, Brain, Cervix, Lung, Lymph, Ovary and Skin.
[9]	Bienvenut W.V., Kanor S., Tissot J.-D., Quadroni M. Submitted (MAY-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-11; 48-57; 107-118; 258-280 AND 309-317, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma and T-cell.
[10]	Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 48-57; 107-118; 140-155 AND 226-245, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[11]	"Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor." Liedtke C.M., Yun C.H.C., Kyle N., Wang D. J. Biol. Chem. 277:22925-22933(2002) [PubMed: 11956211] [Abstract] Cited for: INTERACTION WITH SLC9A3R1.
[12]	"Muscle ring finger protein-1 inhibits PKC-epsilon activation and prevents cardiomyocyte hypertrophy." Arya R., Kedar V., Hwang J.R., McDonough H., Li H.-H., Taylor J., Patterson C. J. Cell Biol. 167:1147-1159(2004) [PubMed: 15596539] [Abstract] Cited for: INTERACTION WITH TRIM63 AND PRKCE.
[13]	"Ki-1/57 interacts with RACK1 and is a substrate for the phosphorylation by phorbol 12-myristate 13-acetate-activated protein kinase C." Nery F.C., Passos D.O., Garcia V.S., Kobarg J. J. Biol. Chem. 279:11444-11455(2004) [PubMed: 14699138] [Abstract] Cited for: INTERACTION WITH HABP4.
[14]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-228, MASS SPECTROMETRY.
[15]	"Interaction of integrin beta1 with cytokeratin 1 in neuroblastoma NMB7 cells." Chuang N.N., Huang C.C. Biochem. Soc. Trans. 35:1292-1294(2007) [PubMed: 17956333] [Abstract] Cited for: FUNCTION, INTERACTION WITH KRT1, SUBCELLULAR LOCATION.
[16]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	M24194 mRNA. Translation: AAA59626.1. AY159316 mRNA. Translation: AAO21313.1. Different initiation. AY336089 mRNA. Translation: AAR24619.1. Frameshift. AK095666 mRNA. Translation: BAG53102.1. CR456978 mRNA. Translation: CAG33259.1. CR541909 mRNA. Translation: CAG46707.1. AK222488 mRNA. Translation: BAD96208.1. CH471165 Genomic DNA. Translation: EAW53692.1. BC000214 mRNA. Translation: AAH00214.1. BC000366 mRNA. Translation: AAH00366.1. BC010119 mRNA. Translation: AAH10119.1. BC014256 mRNA. Translation: AAH14256.1. BC014788 mRNA. Translation: AAH14788.1. BC017287 mRNA. Translation: AAH17287.1. BC019093 mRNA. Translation: AAH19093.1. BC019362 mRNA. Translation: AAH19362.1. BC021993 mRNA. Translation: AAH21993.1. BC032006 mRNA. Translation: AAH32006.1.
IPI	IPI00848226.
PIR	B33928.
RefSeq	NP_006089.1.
UniGene	Hs.5662
3D structure databases
HSSP	HSSP built from PDB template 1ERJ based on UniProtKB P16649.
ModBase	Search...
Protein-protein interaction databases
IntAct	P63244. 17 interactions.
PTM databases
PhosphoSite	P63244.
2-D gel databases
REPRODUCTION-2DPAGE	IPI00848226. P63244.
Proteomic databases
PRIDE	P63244.
Genome annotation databases
Ensembl	ENSG00000204628. Homo sapiens. [Contig view]
GeneID	10399.
KEGG	hsa:10399.
Organism-specific databases
GeneCards	GC05M180596.
H-InvDB	HIX0005509. HIX0080673.
HGNC	HGNC:4399. GNB2L1.
HPA	CAB004288.
MIM	176981. gene.
PharmGKB	PA28779.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P63244.
Enzyme and pathway databases
Pathway_Interaction_DB	hif1apathway. Hypoxic and oxygen homeostasis regulation of HIF-1-alpha. igf1_pathway. IGF1 pathway. ar_tf_pathway. Regulation of Androgen receptor activity. syndecan_2_pathway. Syndecan-2-mediated signaling events. tnfpathway. TNF receptor signaling pathway.
Gene expression databases
ArrayExpress	P63244.
Bgee	P63244.
CleanEx	HS_GNB2L1.
GermOnline	ENSG00000204628. Homo sapiens.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_region. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 7 hits. [Graphical view]
ProDom	PD000018. WD40. 5 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00320. WD40. 7 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 4 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39404.
SOURCE	Search...

Entry information

Entry name

GBLP_HUMAN

Accession

Primary (citable) accession number: P63244
Secondary accession number(s): B3KTJ0 , P25388, P99049, Q53HU2, Q5J8M6, Q5VLR4, Q6FH47

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)