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Reviewed, UniProtKB/Swiss-Prot P63244 (GBLP_HUMAN)

Last modified June 16, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Guanine nucleotide-binding protein subunit beta-2-like 1
Alternative name(s):
    Guanine nucleotide-binding protein subunit beta-like protein 12.3
    Receptor of activated protein kinase C 1
      Short name=RACK1
    Receptor for activated C kinase
    Cell proliferation-inducing gene 21 protein
    Human lung cancer oncogene 7 protein
      Short name=HLC-7
Gene names
Name: GNB2L1
ORF Names: HLC7, PIG21
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation. Ref.15

Subunit structure

Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4 and KRT1. Ref.15 Ref.11 Ref.12 Ref.13

Subcellular location

Cell membrane. Note: Located on plasma membrane of neuroblastoma NMB7 cells. Ref.15

Domain

The WD repeats domain 5 mediates interaction with TRIM63.

Sequence similarities

Belongs to the WD repeat G protein beta family.

Contains 7 WD repeats.

Sequence caution

The sequence AAR24619.1 differs from that shown. Reason: Frameshift at position 94.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainRepeat
WD repeat
   PTMAcetylation
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionprotein phosphatase binding

Inferred from physical interaction. Source: UniProtKB

receptor binding

Non-traceable author statement. Source: UniProtKB

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 317316Guanine nucleotide-binding protein subunit beta-2-like 1
PRO_0000127731

Regions

Repeat13 – 4432WD 1
Repeat61 – 9131WD 2
Repeat103 – 13331WD 3
Repeat146 – 17833WD 4
Repeat190 – 22031WD 5
Repeat231 – 26030WD 6
Repeat281 – 31131WD 7

Amino acid modifications

Modified residue21N-acetylthreonine Ref.9
Modified residue2281Phosphotyrosine Ref.14

Experimental info

Sequence conflict70 – 11142Missing in BAG53102. Ref.4
Sequence conflict941T → TRK in AAR24619. Ref.3
Sequence conflict2211L → F in BAD96208. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P63244-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 257F91E369ED2044

FASTA31735,077
        10         20         30         40         50         60 
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR 

        70         80         90        100        110        120 
GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI 

       130        140        150        160        170        180 
VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA 

       190        200        210        220        230        240 
NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC 

       250        260        270        280        290        300 
FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA 

       310 
GYTDNLVRVW QVTIGTR 

« Hide

References

« Hide 'large scale' references
[1]"Physical linkage of a guanine nucleotide-binding protein-related gene to the chicken major histocompatibility complex."
Guillemot F., Billault A., Auffray C.
Proc. Natl. Acad. Sci. U.S.A. 86:4594-4598(1989) [PubMed: 2499885] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification of new tumor-related gene in human lung cancer."
Kim J.W.
Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Identification of a proliferation-inducing gene."
Kim J.W.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Adipose tissue.
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: B-cell, Brain, Cervix, Lung, Lymph, Ovary and Skin.
[9]Bienvenut W.V., Kanor S., Tissot J.-D., Quadroni M.
Submitted (MAY-2006) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-11; 48-57; 107-118; 258-280 AND 309-317, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, MASS SPECTROMETRY.
Tissue: B-cell lymphoma and T-cell.
[10]Lubec G., Afjehi-Sadat L.
Submitted (MAR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 48-57; 107-118; 140-155 AND 226-245, MASS SPECTROMETRY.
Tissue: Brain and Cajal-Retzius cell.
[11]"Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor."
Liedtke C.M., Yun C.H.C., Kyle N., Wang D.
J. Biol. Chem. 277:22925-22933(2002) [PubMed: 11956211] [Abstract]
Cited for: INTERACTION WITH SLC9A3R1.
[12]"Muscle ring finger protein-1 inhibits PKC-epsilon activation and prevents cardiomyocyte hypertrophy."
Arya R., Kedar V., Hwang J.R., McDonough H., Li H.-H., Taylor J., Patterson C.
J. Cell Biol. 167:1147-1159(2004) [PubMed: 15596539] [Abstract]
Cited for: INTERACTION WITH TRIM63 AND PRKCE.
[13]"Ki-1/57 interacts with RACK1 and is a substrate for the phosphorylation by phorbol 12-myristate 13-acetate-activated protein kinase C."
Nery F.C., Passos D.O., Garcia V.S., Kobarg J.
J. Biol. Chem. 279:11444-11455(2004) [PubMed: 14699138] [Abstract]
Cited for: INTERACTION WITH HABP4.
[14]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-228, MASS SPECTROMETRY.
[15]"Interaction of integrin beta1 with cytokeratin 1 in neuroblastoma NMB7 cells."
Chuang N.N., Huang C.C.
Biochem. Soc. Trans. 35:1292-1294(2007) [PubMed: 17956333] [Abstract]
Cited for: FUNCTION, INTERACTION WITH KRT1, SUBCELLULAR LOCATION.
[16]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

M24194 mRNA. Translation: AAA59626.1.
AY159316 mRNA. Translation: AAO21313.1. Different initiation.
AY336089 mRNA. Translation: AAR24619.1. Frameshift.
AK095666 mRNA. Translation: BAG53102.1.
CR456978 mRNA. Translation: CAG33259.1.
CR541909 mRNA. Translation: CAG46707.1.
AK222488 mRNA. Translation: BAD96208.1.
CH471165 Genomic DNA. Translation: EAW53692.1.
BC000214 mRNA. Translation: AAH00214.1.
BC000366 mRNA. Translation: AAH00366.1.
BC010119 mRNA. Translation: AAH10119.1.
BC014256 mRNA. Translation: AAH14256.1.
BC014788 mRNA. Translation: AAH14788.1.
BC017287 mRNA. Translation: AAH17287.1.
BC019093 mRNA. Translation: AAH19093.1.
BC019362 mRNA. Translation: AAH19362.1.
BC021993 mRNA. Translation: AAH21993.1.
BC032006 mRNA. Translation: AAH32006.1.
IPIIPI00848226.
PIRB33928.
RefSeqNP_006089.1.
UniGeneHs.5662

3D structure databases

HSSPHSSP built from PDB template 1ERJ based on UniProtKB P16649.
ModBaseSearch...

Protein-protein interaction databases

IntActP63244. 17 interactions.

PTM databases

PhosphoSiteP63244.

2-D gel databases

REPRODUCTION-2DPAGEIPI00848226.
P63244.

Proteomic databases

PRIDEP63244.

Genome annotation databases

EnsemblENSG00000204628. Homo sapiens. [Contig view]
GeneID10399.
KEGGhsa:10399.

Organism-specific databases

GeneCardsGC05M180596.
H-InvDBHIX0005509.
HIX0080673.
HGNCHGNC:4399. GNB2L1.
HPACAB004288.
MIM176981. gene.
PharmGKBPA28779.
GenAtlasSearch...

Phylogenomic databases

HOVERGENP63244.

Enzyme and pathway databases

Pathway_Interaction_DBhif1apathway. Hypoxic and oxygen homeostasis regulation of HIF-1-alpha.
igf1_pathway. IGF1 pathway.
ar_tf_pathway. Regulation of Androgen receptor activity.
syndecan_2_pathway. Syndecan-2-mediated signaling events.
tnfpathway. TNF receptor signaling pathway.

Gene expression databases

ArrayExpressP63244.
BgeeP63244.
CleanExHS_GNB2L1.
GermOnlineENSG00000204628. Homo sapiens.

Family and domain databases

InterProIPR015943. WD40/YVTN_repeat-like.
IPR001680. WD40_repeat.
IPR019782. WD40_repeat_2.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_region.
IPR019781. WD40_repeat_sg.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF00400. WD40. 7 hits.
[Graphical view]
ProDomPD000018. WD40. 5 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00320. WD40. 7 hits.
[Graphical view]
PROSITEPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio39404.
SOURCESearch...

Entry information

Entry nameGBLP_HUMAN
AccessionPrimary (citable) accession number: P63244
Secondary accession number(s): B3KTJ0 expand/collapse secondary AC list , P25388, P99049, Q53HU2, Q5J8M6, Q5VLR4, Q6FH47
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 53 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents