P63243 (GBLP_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein subunit beta-2-like 1

Alternative name(s):
Receptor for activated C kinase
Receptor of activated protein kinase C 1
Short name=RACK1

Gene names

Name:

GNB2L1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration By similarity.
Subunit structure	Component of the small (40S) ribosomal subunit. Exists as a monomer and also forms oligomers. Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4, KRT1 and OTUB1. Interacts with SRC (via SH2 domain); the interaction is enhanced by tyrosine phosphorylation of GNB2L1/RACK1. Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and PRKCA. Interacts with AR. Interacts with IGF1R but not with INSR. Interacts with ADAM12. Interacts with CLEC1B (via N-terminal region) and with HIF1A; the interaction promotes their degradation. Interacts with RHOA; this enhances RHOA activation and promotes cell migration. Interacts with CHRM2; the interaction regulates CHRM2 internalization. Interacts with TRPM6 (via kinase domain). Interacts with PTK2/FAK1; required for PTK2/FAK1 phosphorylation and dephosphorylation. Interacts with FLT1. Interacts with HRAS. Interacts with LARP4B By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity. Cytoplasm By similarity. Cytoplasm › perinuclear region By similarity. Cytoplasm › cytoskeleton By similarity. Nucleus By similarity. Perikaryon By similarity. Cell projection › dendrite By similarity. Note: Recruited to the plasma membrane through interaction with KRT1 which binds to membrane-bound ITGB1. PKC activation induces translocation from the perinuclear region to the cell periphery. In the brain, detected mainly in cell bodies and dendrites with little expression in axonal fibers or nuclei By similarity.
Tissue specificity	Expressed in aortic endothelial cells (EC) and the endothelium of tumor neovascularizations. Differential gene expression is displayed in the corpora lutea of the early, mid, and late stages of the ovarian cycle that are associated with progressive, active, and regressive stages of angiogenesis. Ref.1
Post-translational modification	Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC By similarity.
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Apoptosis Biological rhythms Cell cycle Gastrulation Growth regulation Translation regulation
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane Nucleus
Domain	Repeat WD repeat
Molecular function	Developmental protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW gastrulation Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of phagocytosis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of protein kinase B signaling cascade Inferred from electronic annotation. Source: Compara positive regulation of GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell migration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of gastrulation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of intrinsic apoptotic signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell cycle Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell division Inferred from sequence or structural similarity. Source: UniProtKB regulation of establishment of cell polarity Inferred from sequence or structural similarity. Source: UniProtKB regulation of protein localization Inferred from sequence or structural similarity. Source: UniProtKB regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell dendrite Inferred from electronic annotation. Source: UniProtKB-SubCell midbody Inferred from sequence or structural similarity. Source: UniProtKB neuronal cell body Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB perikaryon Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB phagocytic cup Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	SH2 domain binding Inferred from sequence or structural similarity. Source: UniProtKB cysteine-type endopeptidase activator activity involved in apoptotic process Inferred from electronic annotation. Source: Compara ion channel inhibitor activity Inferred from electronic annotation. Source: Compara protein kinase C binding Inferred from sequence or structural similarity. Source: UniProtKB protein tyrosine kinase inhibitor activity Inferred from sequence or structural similarity. Source: UniProtKB receptor tyrosine kinase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 317	316	Guanine nucleotide-binding protein subunit beta-2-like 1		PRO_0000127730
Regions
	Repeat	13 – 44	32	WD 1
	Repeat	61 – 91	31	WD 2
	Repeat	103 – 133	31	WD 3
	Repeat	146 – 178	33	WD 4
	Repeat	190 – 220	31	WD 5
	Repeat	231 – 260	30	WD 6
	Repeat	281 – 311	31	WD 7
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity
	Modified residue	52	1	Phosphotyrosine; by ABL1 By similarity
	Modified residue	130	1	N6-acetyllysine By similarity
	Modified residue	228	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	91	1	D → E in AAI02287. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P63243 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 257F91E369ED2044	FASTA	317	35,077
	10 20 30 40 50 60 MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR 70 80 90 100 110 120 GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI 130 140 150 160 170 180 VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA 190 200 210 220 230 240 NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC 250 260 270 280 290 300 FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA 310 GYTDNLVRVW QVTIGTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RACK1 is up-regulated in angiogenesis and human carcinomas." Berns H., Humar R., Hengerer B., Kiefer F.N., Battegay E.J. FASEB J. 14:2549-2558(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ132860 mRNA. Translation: CAB64792.1. BC102286 mRNA. Translation: AAI02287.2.
IPI	IPI00700792.
RefSeq	NP_786996.1. NM_175802.2.
UniGene	Bt.2981.
3D structure databases
ProteinModelPortal	P63243.
ModBase	Search...
Proteomic databases
PaxDb	P63243.
PRIDE	P63243.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000026183; ENSBTAP00000026183; ENSBTAG00000019648.
GeneID	327682.
KEGG	bta:327682.
Organism-specific databases
CTD	10399.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00700000104461.
HOGENOM	HOG000091643.
HOVERGEN	HBG000277.
InParanoid	P63243.
KO	K14753.
OMA	IRVWQVM.
OrthoDB	EOG4933J5.
Enzyme and pathway databases
BioCyc	CATTLE:327682-MONOMER.
Family and domain databases
Gene3D	2.130.10.10. 2 hits.
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 7 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 4 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20810147.

Entry information

Entry name

GBLP_BOVIN

Accession

Primary (citable) accession number: P63243
Secondary accession number(s): P25388, P99049, Q3T0R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program