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Protein

Receptor of activated protein C kinase 1

Gene

RACK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration (By similarity). Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Apoptosis, Biological rhythms, Cell cycle, Gastrulation, Growth regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-BTA-5357905. Regulation of TNFR1 signaling.
R-BTA-5357956. TNFR1-induced NFkappaB signaling pathway.
R-BTA-5626978. TNFR1-mediated ceramide production.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor of activated protein C kinase 1
Alternative name(s):
Guanine nucleotide-binding protein subunit beta-2-like 1
Receptor for activated C kinase
Receptor of activated protein kinase C 1
Cleaved into the following chain:
Gene namesi
Name:RACK1
Synonyms:GNB2L1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Cytoplasm By similarity
  • Cytoplasmperinuclear region By similarity
  • Nucleus By similarity
  • Perikaryon By similarity
  • Cell projectiondendrite By similarity

  • Note: Recruited to the plasma membrane through interaction with KRT1 which binds to membrane-bound ITGB1. Also associated with the membrane in oncogene-transformed cells. PKC activation induces translocation from the perinuclear region to the cell periphery (By similarity). In the brain, detected mainly in cell bodies and dendrites with little expression in axonal fibers or nuclei (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Receptor of activated protein C kinase 1PRO_0000127730Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 317316Receptor of activated protein C kinase 1, N-terminally processedPRO_0000424479Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylthreonine; in Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processedBy similarity
Modified residuei6 – 61PhosphothreonineBy similarity
Modified residuei10 – 101PhosphothreonineBy similarity
Modified residuei52 – 521Phosphotyrosine; by ABL1By similarity
Modified residuei96 – 961PhosphothreonineBy similarity
Modified residuei130 – 1301N6-acetyllysineBy similarity
Modified residuei183 – 1831N6-acetyllysineBy similarity
Modified residuei228 – 2281PhosphotyrosineBy similarity
Modified residuei276 – 2761PhosphoserineBy similarity
Modified residuei316 – 3161PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required for binding to SRC (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP63243.
PeptideAtlasiP63243.
PRIDEiP63243.

Expressioni

Tissue specificityi

Expressed in aortic endothelial cells (EC) and the endothelium of tumor neovascularizations. Differential gene expression is displayed in the corpora lutea of the early, mid, and late stages of the ovarian cycle that are associated with progressive, active, and regressive stages of angiogenesis.1 Publication

Interactioni

Subunit structurei

Interacts with CPNE3 (By similarity). May interact with ABCB4 (By similarity). Component of the small (40S) ribosomal subunit. Exists as a monomer and also forms oligomers. Binds SLC9A3R1. Forms a ternary complex with TRIM63 and PRKCE. Interacts with HABP4, KRT1 and OTUB1. Interacts with SRC (via SH2 domain); the interaction is enhanced by tyrosine phosphorylation of RACK1. Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and PRKCA. Interacts with AR. Interacts with IGF1R but not with INSR. Interacts with ADAM12. Interacts with CLEC1B (via N-terminal region) and with HIF1A; the interaction promotes their degradation. Interacts with RHOA; this enhances RHOA activation and promotes cell migration. Interacts with CHRM2; the interaction regulates CHRM2 internalization. Interacts with TRPM6 (via kinase domain). Interacts with PTK2/FAK1; required for PTK2/FAK1 phosphorylation and dephosphorylation. Interacts with FLT1. Interacts with HRAS. Interacts with LARP4B. Interacts with PKD2L1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026183.

Structurei

3D structure databases

ProteinModelPortaliP63243.
SMRiP63243. Positions 2-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati13 – 4432WD 1Add
BLAST
Repeati61 – 9131WD 2Add
BLAST
Repeati103 – 13331WD 3Add
BLAST
Repeati146 – 17833WD 4Add
BLAST
Repeati190 – 22031WD 5Add
BLAST
Repeati231 – 26030WD 6Add
BLAST
Repeati281 – 31131WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0279. Eukaryota.
ENOG410XQGZ. LUCA.
GeneTreeiENSGT00840000129847.
HOGENOMiHOG000091643.
HOVERGENiHBG000277.
InParanoidiP63243.
KOiK14753.
OMAiATSMENP.
OrthoDBiEOG7BW0JV.
TreeFamiTF300600.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET
60 70 80 90 100
NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR
110 120 130 140 150
FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW
160 170 180 190 200
VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA NCKLKTNHIG HTGYLNTVTV
210 220 230 240 250
SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA
260 270 280 290 300
ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA
310
GYTDNLVRVW QVTIGTR
Length:317
Mass (Da):35,077
Last modified:January 23, 2007 - v3
Checksum:i257F91E369ED2044
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911D → E in AAI02287 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132860 mRNA. Translation: CAB64792.1.
BC102286 mRNA. Translation: AAI02287.2.
RefSeqiNP_786996.1. NM_175802.3.
UniGeneiBt.2981.

Genome annotation databases

EnsembliENSBTAT00000026183; ENSBTAP00000026183; ENSBTAG00000019648.
GeneIDi327682.
KEGGibta:327682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132860 mRNA. Translation: CAB64792.1.
BC102286 mRNA. Translation: AAI02287.2.
RefSeqiNP_786996.1. NM_175802.3.
UniGeneiBt.2981.

3D structure databases

ProteinModelPortaliP63243.
SMRiP63243. Positions 2-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026183.

Proteomic databases

PaxDbiP63243.
PeptideAtlasiP63243.
PRIDEiP63243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026183; ENSBTAP00000026183; ENSBTAG00000019648.
GeneIDi327682.
KEGGibta:327682.

Organism-specific databases

CTDi10399.

Phylogenomic databases

eggNOGiKOG0279. Eukaryota.
ENOG410XQGZ. LUCA.
GeneTreeiENSGT00840000129847.
HOGENOMiHOG000091643.
HOVERGENiHBG000277.
InParanoidiP63243.
KOiK14753.
OMAiATSMENP.
OrthoDBiEOG7BW0JV.
TreeFamiTF300600.

Enzyme and pathway databases

ReactomeiR-BTA-5357905. Regulation of TNFR1 signaling.
R-BTA-5357956. TNFR1-induced NFkappaB signaling pathway.
R-BTA-5626978. TNFR1-mediated ceramide production.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RACK1 is up-regulated in angiogenesis and human carcinomas."
    Berns H., Humar R., Hengerer B., Kiefer F.N., Battegay E.J.
    FASEB J. 14:2549-2558(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiRACK1_BOVIN
AccessioniPrimary (citable) accession number: P63243
Secondary accession number(s): P25388, P99049, Q3T0R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.