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Protein

Eukaryotic translation initiation factor 5A-1

Gene

Eif5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

mRNA transport, Protein biosynthesis, Protein transport, Translocation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-204626. Hypusine synthesis from eIF5A-lysine.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5A-1
Short name:
eIF-5A-1
Short name:
eIF-5A1
Alternative name(s):
Eukaryotic initiation factor 5A isoform 1
Short name:
eIF-5A
eIF-4D
Gene namesi
Name:Eif5a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:106248. Eif5a.

Subcellular locationi

GO - Cellular componenti

  • annulate lamellae Source: MGI
  • cytoplasm Source: MGI
  • dendrite Source: Ensembl
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • neuronal cell body Source: Ensembl
  • nuclear pore Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001424522 – 154Eukaryotic translation initiation factor 5A-1Add BLAST153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei47N6-acetyllysineBy similarity1
Modified residuei50HypusineBy similarity1
Modified residuei121N6-acetyllysineCombined sources1

Post-translational modificationi

Acetylated. Deacetylated by SIRT2 (By similarity).By similarity
eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Keywords - PTMi

Acetylation, Hypusine

Proteomic databases

EPDiP63242.
MaxQBiP63242.
PaxDbiP63242.
PeptideAtlasiP63242.
PRIDEiP63242.
TopDownProteomicsiP63242.

2D gel databases

REPRODUCTION-2DPAGEP63242.
UCD-2DPAGEP63242.

PTM databases

iPTMnetiP63242.
PhosphoSitePlusiP63242.
SwissPalmiP63242.

Expressioni

Gene expression databases

BgeeiENSMUSG00000078812.
CleanExiMM_EIF5A.
ExpressionAtlasiP63242. baseline and differential.
GenevisibleiP63242. MM.

Interactioni

Subunit structurei

Interacts with DHPS, with SDCBP and DOHH. Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234898. 3 interactors.
IntActiP63242. 5 interactors.
MINTiMINT-1530847.
STRINGi10090.ENSMUSP00000047008.

Structurei

3D structure databases

ProteinModelPortaliP63242.
SMRiP63242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 19Nuclear localization regulationAdd BLAST18

Sequence similaritiesi

Belongs to the eIF-5A family.Curated

Phylogenomic databases

eggNOGiKOG3271. Eukaryota.
COG0231. LUCA.
GeneTreeiENSGT00390000003738.
HOGENOMiHOG000106270.
HOVERGENiHBG001104.
InParanoidiP63242.
KOiK03263.
OMAiIVEISTT.
OrthoDBiEOG091G0P2P.
PhylomeDBiP63242.
TreeFamiTF101534.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR11673. PTHR11673. 1 hit.
PfamiPF01287. eIF-5a. 1 hit.
[Graphical view]
PIRSFiPIRSF003025. eIF5A. 1 hit.
SMARTiSM01376. eIF-5a. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK
60 70 80 90 100
HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS
110 120 130 140 150
LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK

AMAK
Length:154
Mass (Da):16,832
Last modified:January 23, 2007 - v2
Checksum:i07EF043C7DEA3091
GO

Sequence cautioni

The sequence CAI35154 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8E → Y in BAB27641 (PubMed:16141072).Curated1
Sequence conflicti16A → S in BAB27641 (PubMed:16141072).Curated1
Sequence conflicti139S → F in BAB27641 (PubMed:16141072).Curated1
Sequence conflicti143E → K in BAB27641 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY129323 mRNA. Translation: AAN17521.1.
AY129324 mRNA. Translation: AAN17527.1.
AY129325 mRNA. Translation: AAN17528.1.
AY129326 mRNA. Translation: AAN17532.1.
AY129327 mRNA. Translation: AAN17534.1.
AY129328 mRNA. Translation: AAN17535.1.
AY129329 mRNA. Translation: AAN17539.1.
AK011306 mRNA. Translation: BAB27532.1.
AK011470 mRNA. Translation: BAB27641.1.
AK149705 mRNA. Translation: BAE29039.1.
AL596185 Genomic DNA. Translation: CAI35153.1.
AL596185 Genomic DNA. Translation: CAI35154.1. Sequence problems.
BC003889 mRNA. Translation: AAH03889.1.
BC008093 mRNA. Translation: AAH08093.1.
BC024899 mRNA. Translation: AAH24899.1.
CCDSiCCDS24923.1.
RefSeqiNP_001160061.1. NM_001166589.1.
NP_001160062.1. NM_001166590.1.
NP_001160063.1. NM_001166591.1.
NP_001160064.1. NM_001166592.1.
NP_001160065.1. NM_001166593.1.
NP_001160066.1. NM_001166594.1.
NP_001160067.1. NM_001166595.1.
NP_001160068.1. NM_001166596.1.
NP_853613.1. NM_181582.4.
XP_011247360.1. XM_011249058.2.
UniGeneiMm.18026.
Mm.486207.

Genome annotation databases

EnsembliENSMUST00000043419; ENSMUSP00000047008; ENSMUSG00000078812.
ENSMUST00000070996; ENSMUSP00000067077; ENSMUSG00000078812.
ENSMUST00000071026; ENSMUSP00000068651; ENSMUSG00000078812.
ENSMUST00000108607; ENSMUSP00000104247; ENSMUSG00000078812.
ENSMUST00000108608; ENSMUSP00000104248; ENSMUSG00000078812.
ENSMUST00000108609; ENSMUSP00000104249; ENSMUSG00000078812.
ENSMUST00000108610; ENSMUSP00000104250; ENSMUSG00000078812.
ENSMUST00000108611; ENSMUSP00000104251; ENSMUSG00000078812.
ENSMUST00000108612; ENSMUSP00000104252; ENSMUSG00000078812.
ENSMUST00000108613; ENSMUSP00000104253; ENSMUSG00000078812.
ENSMUST00000164359; ENSMUSP00000132717; ENSMUSG00000078812.
GeneIDi276770.
KEGGimmu:276770.
UCSCiuc007jsq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY129323 mRNA. Translation: AAN17521.1.
AY129324 mRNA. Translation: AAN17527.1.
AY129325 mRNA. Translation: AAN17528.1.
AY129326 mRNA. Translation: AAN17532.1.
AY129327 mRNA. Translation: AAN17534.1.
AY129328 mRNA. Translation: AAN17535.1.
AY129329 mRNA. Translation: AAN17539.1.
AK011306 mRNA. Translation: BAB27532.1.
AK011470 mRNA. Translation: BAB27641.1.
AK149705 mRNA. Translation: BAE29039.1.
AL596185 Genomic DNA. Translation: CAI35153.1.
AL596185 Genomic DNA. Translation: CAI35154.1. Sequence problems.
BC003889 mRNA. Translation: AAH03889.1.
BC008093 mRNA. Translation: AAH08093.1.
BC024899 mRNA. Translation: AAH24899.1.
CCDSiCCDS24923.1.
RefSeqiNP_001160061.1. NM_001166589.1.
NP_001160062.1. NM_001166590.1.
NP_001160063.1. NM_001166591.1.
NP_001160064.1. NM_001166592.1.
NP_001160065.1. NM_001166593.1.
NP_001160066.1. NM_001166594.1.
NP_001160067.1. NM_001166595.1.
NP_001160068.1. NM_001166596.1.
NP_853613.1. NM_181582.4.
XP_011247360.1. XM_011249058.2.
UniGeneiMm.18026.
Mm.486207.

3D structure databases

ProteinModelPortaliP63242.
SMRiP63242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234898. 3 interactors.
IntActiP63242. 5 interactors.
MINTiMINT-1530847.
STRINGi10090.ENSMUSP00000047008.

PTM databases

iPTMnetiP63242.
PhosphoSitePlusiP63242.
SwissPalmiP63242.

2D gel databases

REPRODUCTION-2DPAGEP63242.
UCD-2DPAGEP63242.

Proteomic databases

EPDiP63242.
MaxQBiP63242.
PaxDbiP63242.
PeptideAtlasiP63242.
PRIDEiP63242.
TopDownProteomicsiP63242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043419; ENSMUSP00000047008; ENSMUSG00000078812.
ENSMUST00000070996; ENSMUSP00000067077; ENSMUSG00000078812.
ENSMUST00000071026; ENSMUSP00000068651; ENSMUSG00000078812.
ENSMUST00000108607; ENSMUSP00000104247; ENSMUSG00000078812.
ENSMUST00000108608; ENSMUSP00000104248; ENSMUSG00000078812.
ENSMUST00000108609; ENSMUSP00000104249; ENSMUSG00000078812.
ENSMUST00000108610; ENSMUSP00000104250; ENSMUSG00000078812.
ENSMUST00000108611; ENSMUSP00000104251; ENSMUSG00000078812.
ENSMUST00000108612; ENSMUSP00000104252; ENSMUSG00000078812.
ENSMUST00000108613; ENSMUSP00000104253; ENSMUSG00000078812.
ENSMUST00000164359; ENSMUSP00000132717; ENSMUSG00000078812.
GeneIDi276770.
KEGGimmu:276770.
UCSCiuc007jsq.2. mouse.

Organism-specific databases

CTDi1984.
MGIiMGI:106248. Eif5a.

Phylogenomic databases

eggNOGiKOG3271. Eukaryota.
COG0231. LUCA.
GeneTreeiENSGT00390000003738.
HOGENOMiHOG000106270.
HOVERGENiHBG001104.
InParanoidiP63242.
KOiK03263.
OMAiIVEISTT.
OrthoDBiEOG091G0P2P.
PhylomeDBiP63242.
TreeFamiTF101534.

Enzyme and pathway databases

ReactomeiR-MMU-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

ChiTaRSiEif5a. mouse.
PROiP63242.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078812.
CleanExiMM_EIF5A.
ExpressionAtlasiP63242. baseline and differential.
GenevisibleiP63242. MM.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR11673. PTHR11673. 1 hit.
PfamiPF01287. eIF-5a. 1 hit.
[Graphical view]
PIRSFiPIRSF003025. eIF5A. 1 hit.
SMARTiSM01376. eIF-5a. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF5A1_MOUSE
AccessioniPrimary (citable) accession number: P63242
Secondary accession number(s): P10159
, Q16182, Q5NCX0, Q78NR3, Q9D0G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.