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Protein

Eukaryotic translation initiation factor 5A-1

Gene

Eif5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionElongation factor, RNA-binding
Biological processmRNA transport, Protein biosynthesis, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-204626 Hypusine synthesis from eIF5A-lysine

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5A-1
Short name:
eIF-5A-1
Short name:
eIF-5A1
Alternative name(s):
Eukaryotic initiation factor 5A isoform 1
Short name:
eIF-5A
eIF-4D
Gene namesi
Name:Eif5a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:106248 Eif5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001424522 – 154Eukaryotic translation initiation factor 5A-1Add BLAST153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei47N6-acetyllysineBy similarity1
Modified residuei50HypusineBy similarity1
Modified residuei121N6-acetyllysineCombined sources1

Post-translational modificationi

Acetylated. Deacetylated by SIRT2 (By similarity).By similarity
eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Keywords - PTMi

Acetylation, Hypusine

Proteomic databases

EPDiP63242
MaxQBiP63242
PaxDbiP63242
PeptideAtlasiP63242
PRIDEiP63242
TopDownProteomicsiP63242

2D gel databases

REPRODUCTION-2DPAGEP63242
UCD-2DPAGEP63242

PTM databases

iPTMnetiP63242
PhosphoSitePlusiP63242
SwissPalmiP63242

Expressioni

Gene expression databases

BgeeiENSMUSG00000078812
CleanExiMM_EIF5A
ExpressionAtlasiP63242 baseline and differential
GenevisibleiP63242 MM

Interactioni

Subunit structurei

Interacts with DHPS, with SDCBP and DOHH. Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234898, 3 interactors
IntActiP63242, 6 interactors
MINTiP63242
STRINGi10090.ENSMUSP00000047008

Structurei

3D structure databases

ProteinModelPortaliP63242
SMRiP63242
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 19Nuclear localization regulationAdd BLAST18

Sequence similaritiesi

Belongs to the eIF-5A family.Curated

Phylogenomic databases

eggNOGiKOG3271 Eukaryota
COG0231 LUCA
GeneTreeiENSGT00390000003738
HOGENOMiHOG000106270
HOVERGENiHBG001104
InParanoidiP63242
KOiK03263
OMAiCVIAIKT
OrthoDBiEOG091G0P2P
PhylomeDBiP63242
TreeFamiTF101534

Family and domain databases

Gene3Di2.30.30.30, 1 hit
InterProiView protein in InterPro
IPR001884 IF5A-like
IPR012340 NA-bd_OB-fold
IPR014722 Rib_L2_dom2
IPR019769 Trans_elong_IF5A_hypusine_site
IPR020189 Transl_elong_IF5A_C
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR11673 PTHR11673, 1 hit
PfamiView protein in Pfam
PF01287 eIF-5a, 1 hit
PIRSFiPIRSF003025 eIF5A, 1 hit
SMARTiView protein in SMART
SM01376 eIF-5a, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00037 eIF_5A, 1 hit
PROSITEiView protein in PROSITE
PS00302 IF5A_HYPUSINE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK
60 70 80 90 100
HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS
110 120 130 140 150
LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK

AMAK
Length:154
Mass (Da):16,832
Last modified:January 23, 2007 - v2
Checksum:i07EF043C7DEA3091
GO

Sequence cautioni

The sequence CAI35154 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8E → Y in BAB27641 (PubMed:16141072).Curated1
Sequence conflicti16A → S in BAB27641 (PubMed:16141072).Curated1
Sequence conflicti139S → F in BAB27641 (PubMed:16141072).Curated1
Sequence conflicti143E → K in BAB27641 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY129323 mRNA Translation: AAN17521.1
AY129324 mRNA Translation: AAN17527.1
AY129325 mRNA Translation: AAN17528.1
AY129326 mRNA Translation: AAN17532.1
AY129327 mRNA Translation: AAN17534.1
AY129328 mRNA Translation: AAN17535.1
AY129329 mRNA Translation: AAN17539.1
AK011306 mRNA Translation: BAB27532.1
AK011470 mRNA Translation: BAB27641.1
AK149705 mRNA Translation: BAE29039.1
AL596185 Genomic DNA Translation: CAI35153.1
AL596185 Genomic DNA Translation: CAI35154.1 Sequence problems.
BC003889 mRNA Translation: AAH03889.1
BC008093 mRNA Translation: AAH08093.1
BC024899 mRNA Translation: AAH24899.1
CCDSiCCDS24923.1
RefSeqiNP_001160061.1, NM_001166589.1
NP_001160062.1, NM_001166590.1
NP_001160063.1, NM_001166591.1
NP_001160064.1, NM_001166592.1
NP_001160065.1, NM_001166593.1
NP_001160066.1, NM_001166594.1
NP_001160067.1, NM_001166595.1
NP_001160068.1, NM_001166596.1
NP_853613.1, NM_181582.4
XP_011247360.1, XM_011249058.2
UniGeneiMm.18026
Mm.486207

Genome annotation databases

EnsembliENSMUST00000043419; ENSMUSP00000047008; ENSMUSG00000078812
ENSMUST00000070996; ENSMUSP00000067077; ENSMUSG00000078812
ENSMUST00000071026; ENSMUSP00000068651; ENSMUSG00000078812
ENSMUST00000108607; ENSMUSP00000104247; ENSMUSG00000078812
ENSMUST00000108608; ENSMUSP00000104248; ENSMUSG00000078812
ENSMUST00000108609; ENSMUSP00000104249; ENSMUSG00000078812
ENSMUST00000108610; ENSMUSP00000104250; ENSMUSG00000078812
ENSMUST00000108611; ENSMUSP00000104251; ENSMUSG00000078812
ENSMUST00000108612; ENSMUSP00000104252; ENSMUSG00000078812
ENSMUST00000108613; ENSMUSP00000104253; ENSMUSG00000078812
ENSMUST00000164359; ENSMUSP00000132717; ENSMUSG00000078812
GeneIDi276770
KEGGimmu:276770
UCSCiuc007jsq.2 mouse

Similar proteinsi

Entry informationi

Entry nameiIF5A1_MOUSE
AccessioniPrimary (citable) accession number: P63242
Secondary accession number(s): P10159
, Q16182, Q5NCX0, Q78NR3, Q9D0G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health