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Protein

Eukaryotic translation initiation factor 5A-1

Gene

EIF5A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.5 Publications

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • protein N-terminus binding Source: UniProtKB
  • ribosome binding Source: InterPro
  • RNA binding Source: UniProtKB
  • translation elongation factor activity Source: UniProtKB
  • U6 snRNA binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • mRNA export from nucleus Source: UniProtKB
  • nucleocytoplasmic transport Source: UniProtKB
  • peptidyl-lysine modification to peptidyl-hypusine Source: Reactome
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of translational elongation Source: UniProtKB
  • positive regulation of translational termination Source: InterPro
  • protein export from nucleus Source: UniProtKB
  • translational frameshifting Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

mRNA transport, Protein biosynthesis, Protein transport, Translocation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132507-MONOMER.
ReactomeiR-HSA-204626. Hypusine synthesis from eIF5A-lysine.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5A-1
Short name:
eIF-5A-1
Short name:
eIF-5A1
Alternative name(s):
Eukaryotic initiation factor 5A isoform 1
Short name:
eIF-5A
Rev-binding factor
eIF-4D
Gene namesi
Name:EIF5A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:3300. EIF5A.

Subcellular locationi

GO - Cellular componenti

  • annulate lamellae Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • nuclear pore Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Biotechnological usei

Mature eIF5A-1 may be used as an in situ diagnostic marker for aberrant proliferation in intraepithelial neoplasia of the vulva.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47K → A or R: Abolishes acetylation. 2 Publications1
Mutagenesisi47K → D: Causes total inactivation of eIF5A in supporting yeast growth. 2 Publications1
Mutagenesisi49G → A: Causes total inactivation of eIF5A in supporting yeast growth. 1 Publication1
Mutagenesisi50K → A: Decreases significantly the acetylation at position K-47 and causes total inactivation of eIF5A in supporting yeast growth. 3 Publications1
Mutagenesisi50K → I, D or R: Causes total inactivation of eIF5A in supporting yeast growth. 3 Publications1
Mutagenesisi52G → A: Causes total inactivation of eIF5A in supporting yeast growth. 1 Publication1
Mutagenesisi55K → A: Causes total inactivation of eIF5A in supporting yeast growth. 1 Publication1
Mutagenesisi81V → G: Leads to temperature sensitivity when expressed in yeast cells. 1 Publication1

Organism-specific databases

DisGeNETi1984.
OpenTargetsiENSG00000132507.
PharmGKBiPA27726.

Polymorphism and mutation databases

BioMutaiEIF5A.
DMDMi54037409.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001424512 – 154Eukaryotic translation initiation factor 5A-1Add BLAST153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei47N6-acetyllysine1 Publication1
Modified residuei50Hypusine1 Publication1
Modified residuei121N6-acetyllysineBy similarity1

Post-translational modificationi

Acetylated. Deacetylated by SIRT2.3 Publications
eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Keywords - PTMi

Acetylation, Hypusine

Proteomic databases

EPDiP63241.
MaxQBiP63241.
PaxDbiP63241.
PeptideAtlasiP63241.
PRIDEiP63241.
TopDownProteomicsiP63241-1. [P63241-1]
P63241-2. [P63241-2]

2D gel databases

DOSAC-COBS-2DPAGEP63241.
OGPiP63241.

PTM databases

iPTMnetiP63241.
PhosphoSitePlusiP63241.
SwissPalmiP63241.

Miscellaneous databases

PMAP-CutDBP63241.

Expressioni

Tissue specificityi

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000132507.
CleanExiHS_EIF5A.
ExpressionAtlasiP63241. baseline and differential.
GenevisibleiP63241. HS.

Organism-specific databases

HPAiCAB005042.
HPA061298.

Interactioni

Subunit structurei

Interacts with DHPS, with SDCBP and DOHH. Interacts with HIV-1 protein Rev. Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CRXO431863EBI-373150,EBI-748171
DHPSP493663EBI-373150,EBI-741925
MEOX2A4D1275EBI-373150,EBI-10172134
RELQ048643EBI-373150,EBI-307352

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108299. 46 interactors.
IntActiP63241. 27 interactors.
MINTiMINT-1829069.
STRINGi9606.ENSP00000336702.

Structurei

Secondary structure

1154
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi17 – 21Combined sources5
Helixi22 – 24Combined sources3
Beta strandi29 – 33Combined sources5
Beta strandi36 – 46Combined sources11
Beta strandi55 – 62Combined sources8
Turni63 – 65Combined sources3
Beta strandi68 – 74Combined sources7
Beta strandi77 – 82Combined sources6
Beta strandi85 – 95Combined sources11
Beta strandi98 – 102Combined sources5
Helixi117 – 129Combined sources13
Beta strandi134 – 140Combined sources7
Beta strandi143 – 150Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FH4model-A1-154[»]
3CPFX-ray2.50A/B15-151[»]
5DLQX-ray3.20E/F15-154[»]
ProteinModelPortaliP63241.
SMRiP63241.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63241.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 90DOHH-bindingAdd BLAST71

Sequence similaritiesi

Belongs to the eIF-5A family.Curated

Phylogenomic databases

eggNOGiKOG3271. Eukaryota.
COG0231. LUCA.
GeneTreeiENSGT00390000003738.
HOVERGENiHBG001104.
InParanoidiP63241.
KOiK03263.
OMAiIVEISTT.
PhylomeDBiP63241.
TreeFamiTF101534.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR11673. PTHR11673. 1 hit.
PfamiPF01287. eIF-5a. 1 hit.
[Graphical view]
PIRSFiPIRSF003025. eIF5A. 1 hit.
SMARTiSM01376. eIF-5a. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P63241-1) [UniParc]FASTAAdd to basket
Also known as: B, C, D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK
60 70 80 90 100
HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS
110 120 130 140 150
LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK

AMAK
Length:154
Mass (Da):16,832
Last modified:January 23, 2007 - v2
Checksum:i07EF043C7DEA3091
GO
Isoform 2 (identifier: P63241-2) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MA → MCGTGGTDSKTRRPPHRASFLKRLESKPLKMA

Show »
Length:184
Mass (Da):20,170
Checksum:i9E2A400E66540B74
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36R → W in AAD14095 (PubMed:7545941).Curated1
Sequence conflicti45T → A in AAD14095 (PubMed:7545941).Curated1
Sequence conflicti85K → R in AAD14095 (PubMed:7545941).Curated1
Sequence conflicti109R → P in AAD14095 (PubMed:7545941).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0220201 – 2MA → MCGTGGTDSKTRRPPHRASF LKRLESKPLKMA in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23419 mRNA. Translation: AAA58453.1.
S72024 Genomic DNA. Translation: AAD14095.1.
U17969 Genomic DNA. Translation: AAA86989.1.
AY129319 mRNA. Translation: AAN17514.1.
AY129320 mRNA. Translation: AAN17515.1.
AY129321 mRNA. Translation: AAN17516.1.
AY129322 mRNA. Translation: AAN17518.1.
AK292615 mRNA. Translation: BAF85304.1.
CH471108 Genomic DNA. Translation: EAW90219.1.
CH471108 Genomic DNA. Translation: EAW90220.1.
CH471108 Genomic DNA. Translation: EAW90221.1.
CH471108 Genomic DNA. Translation: EAW90222.1.
BC000751 mRNA. Translation: AAH00751.1.
BC001832 mRNA. Translation: AAH01832.1.
BC030160 mRNA. Translation: AAH30160.1.
BC080196 mRNA. Translation: AAH80196.1.
BC085015 mRNA. Translation: AAH85015.1.
BC107779 mRNA. Translation: AAI07780.1.
CCDSiCCDS11099.1. [P63241-1]
CCDS45601.1. [P63241-2]
PIRiB31486. FIHUA.
RefSeqiNP_001137232.1. NM_001143760.1. [P63241-2]
NP_001137233.1. NM_001143761.1. [P63241-1]
NP_001137234.1. NM_001143762.1. [P63241-1]
NP_001961.1. NM_001970.4. [P63241-1]
XP_005256566.1. XM_005256509.2. [P63241-1]
UniGeneiHs.104825.
Hs.534314.

Genome annotation databases

EnsembliENST00000336452; ENSP00000336702; ENSG00000132507. [P63241-2]
ENST00000336458; ENSP00000336776; ENSG00000132507. [P63241-1]
ENST00000416016; ENSP00000396073; ENSG00000132507. [P63241-1]
ENST00000419711; ENSP00000390677; ENSG00000132507. [P63241-1]
ENST00000571955; ENSP00000458269; ENSG00000132507. [P63241-1]
ENST00000573542; ENSP00000459611; ENSG00000132507. [P63241-1]
ENST00000576930; ENSP00000459196; ENSG00000132507. [P63241-1]
GeneIDi1984.
KEGGihsa:1984.
UCSCiuc002gfr.3. human. [P63241-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23419 mRNA. Translation: AAA58453.1.
S72024 Genomic DNA. Translation: AAD14095.1.
U17969 Genomic DNA. Translation: AAA86989.1.
AY129319 mRNA. Translation: AAN17514.1.
AY129320 mRNA. Translation: AAN17515.1.
AY129321 mRNA. Translation: AAN17516.1.
AY129322 mRNA. Translation: AAN17518.1.
AK292615 mRNA. Translation: BAF85304.1.
CH471108 Genomic DNA. Translation: EAW90219.1.
CH471108 Genomic DNA. Translation: EAW90220.1.
CH471108 Genomic DNA. Translation: EAW90221.1.
CH471108 Genomic DNA. Translation: EAW90222.1.
BC000751 mRNA. Translation: AAH00751.1.
BC001832 mRNA. Translation: AAH01832.1.
BC030160 mRNA. Translation: AAH30160.1.
BC080196 mRNA. Translation: AAH80196.1.
BC085015 mRNA. Translation: AAH85015.1.
BC107779 mRNA. Translation: AAI07780.1.
CCDSiCCDS11099.1. [P63241-1]
CCDS45601.1. [P63241-2]
PIRiB31486. FIHUA.
RefSeqiNP_001137232.1. NM_001143760.1. [P63241-2]
NP_001137233.1. NM_001143761.1. [P63241-1]
NP_001137234.1. NM_001143762.1. [P63241-1]
NP_001961.1. NM_001970.4. [P63241-1]
XP_005256566.1. XM_005256509.2. [P63241-1]
UniGeneiHs.104825.
Hs.534314.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FH4model-A1-154[»]
3CPFX-ray2.50A/B15-151[»]
5DLQX-ray3.20E/F15-154[»]
ProteinModelPortaliP63241.
SMRiP63241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108299. 46 interactors.
IntActiP63241. 27 interactors.
MINTiMINT-1829069.
STRINGi9606.ENSP00000336702.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP63241.
PhosphoSitePlusiP63241.
SwissPalmiP63241.

Polymorphism and mutation databases

BioMutaiEIF5A.
DMDMi54037409.

2D gel databases

DOSAC-COBS-2DPAGEP63241.
OGPiP63241.

Proteomic databases

EPDiP63241.
MaxQBiP63241.
PaxDbiP63241.
PeptideAtlasiP63241.
PRIDEiP63241.
TopDownProteomicsiP63241-1. [P63241-1]
P63241-2. [P63241-2]

Protocols and materials databases

DNASUi1984.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336452; ENSP00000336702; ENSG00000132507. [P63241-2]
ENST00000336458; ENSP00000336776; ENSG00000132507. [P63241-1]
ENST00000416016; ENSP00000396073; ENSG00000132507. [P63241-1]
ENST00000419711; ENSP00000390677; ENSG00000132507. [P63241-1]
ENST00000571955; ENSP00000458269; ENSG00000132507. [P63241-1]
ENST00000573542; ENSP00000459611; ENSG00000132507. [P63241-1]
ENST00000576930; ENSP00000459196; ENSG00000132507. [P63241-1]
GeneIDi1984.
KEGGihsa:1984.
UCSCiuc002gfr.3. human. [P63241-1]

Organism-specific databases

CTDi1984.
DisGeNETi1984.
GeneCardsiEIF5A.
HGNCiHGNC:3300. EIF5A.
HPAiCAB005042.
HPA061298.
MIMi600187. gene.
neXtProtiNX_P63241.
OpenTargetsiENSG00000132507.
PharmGKBiPA27726.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3271. Eukaryota.
COG0231. LUCA.
GeneTreeiENSGT00390000003738.
HOVERGENiHBG001104.
InParanoidiP63241.
KOiK03263.
OMAiIVEISTT.
PhylomeDBiP63241.
TreeFamiTF101534.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132507-MONOMER.
ReactomeiR-HSA-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

ChiTaRSiEIF5A. human.
EvolutionaryTraceiP63241.
GeneWikiiEIF5A.
GenomeRNAii1984.
PMAP-CutDBP63241.
PROiP63241.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132507.
CleanExiHS_EIF5A.
ExpressionAtlasiP63241. baseline and differential.
GenevisibleiP63241. HS.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR014722. Rib_L2_dom2.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR020189. Transl_elong_IF5A_C.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR11673. PTHR11673. 1 hit.
PfamiPF01287. eIF-5a. 1 hit.
[Graphical view]
PIRSFiPIRSF003025. eIF5A. 1 hit.
SMARTiSM01376. eIF-5a. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00037. eIF_5A. 1 hit.
PROSITEiPS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF5A1_HUMAN
AccessioniPrimary (citable) accession number: P63241
Secondary accession number(s): A8K9A0
, D3DTP2, P10159, Q16182, Q7L7L3, Q7Z4L1, Q9D0G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.