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P63240

- NEC1_MUSCO

UniProt

P63240 - NEC1_MUSCO

Protein

Neuroendocrine convertase 1

Gene

Pcsk1

Organism
Mus cookii (Cook's mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

    Catalytic activityi

    Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei167 – 1671Charge relay systemBy similarity
    Active sitei208 – 2081Charge relay systemBy similarity
    Active sitei382 – 3821Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Ligandi

    Calcium

    Protein family/group databases

    MEROPSiS08.072.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuroendocrine convertase 1 (EC:3.4.21.93)
    Short name:
    NEC 1
    Alternative name(s):
    Furin homolog
    PC3
    Prohormone convertase 1
    Propeptide-processing protease
    Proprotein convertase 1
    Short name:
    PC1
    Gene namesi
    Name:Pcsk1
    Synonyms:Att-1, Nec-1, Nec1
    OrganismiMus cookii (Cook's mouse)
    Taxonomic identifieri10098 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

    Organism-specific databases

    MGIiMGI:97511. Pcsk1.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesicle
    Note: Localized in the secretion granules.

    GO - Cellular componenti

    1. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Propeptidei28 – 11083Sequence AnalysisPRO_0000027061Add
    BLAST
    Chaini111 – 753643Neuroendocrine convertase 1PRO_0000027062Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi225 ↔ 374By similarity
    Disulfide bondi317 ↔ 347By similarity
    Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi467 ↔ 494By similarity
    Glycosylationi645 – 6451N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP63240.
    SMRiP63240. Positions 31-103, 122-597.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini162 – 451290Peptidase S8Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S8 family. Furin subfamily.Curated
    Contains 1 peptidase S8 domain.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG008705.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR022005. Proho_convert.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view]
    PANTHERiPTHR10795. PTHR10795. 1 hit.
    PfamiPF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    PF12177. Proho_convert. 1 hit.
    [Graphical view]
    PRINTSiPR00723. SUBTILISIN.
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P63240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQRGWTLQC TAFAFFCVWC ALSSVKAKRQ FVNEWAAEIP GGQEAASAIA    50
    EELGYDLLGQ IGSLENHYLF KHKSHPRRSR RSALHITKRL SDDDRVTWAE 100
    QQYEKERSKR SVQKDSALDL FNDPMWNQQW YLQDTRMTAA LPKLDLHVIP 150
    VWEKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY 200
    DLTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE 250
    ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG 300
    SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST 350
    LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP 400
    NLTWRDMQHL VVWTSEYDPL ASNPGWKKNG AGLMVNSRFG FGLLNAKALV 450
    DLADPRTWRN VPEKKECVVK DNNFEPRALK ANGEVIVEIP TRACEGQENA 500
    IKSLEHVQFE ATIEYSRRGD LHVTLTSAVG TSTVLLAERE RDTSPNGFKN 550
    WDFMSVHTWG ENPVGTWTLK ITDMSGRMQN EGRIVNWKLI LHGTSSQPEH 600
    MKQPRVYTSY NTVQNDRRGV EKMVNVVEKR PTQKSLNGNL LVPKNSSSSN 650
    VEGRRDEQVQ GTPSKAMLRL LQSAFSKNAL SKQSPKKSPS AKLSIPYESF 700
    YEALEKLNKP SKLEGSEDSL YSDYVDVFYN TKPYKHRDDR LLQALMDILN 750
    EEN 753
    Length:753
    Mass (Da):84,147
    Last modified:October 11, 2004 - v1
    Checksum:i95B78441BE8BD9CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58507 mRNA. Translation: AAA39896.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58507 mRNA. Translation: AAA39896.1 .

    3D structure databases

    ProteinModelPortali P63240.
    SMRi P63240. Positions 31-103, 122-597.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S08.072.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:97511. Pcsk1.

    Phylogenomic databases

    HOVERGENi HBG008705.

    Miscellaneous databases

    NextBioi 294338.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR022005. Proho_convert.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view ]
    PANTHERi PTHR10795. PTHR10795. 1 hit.
    Pfami PF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    PF12177. Proho_convert. 1 hit.
    [Graphical view ]
    PRINTSi PR00723. SUBTILISIN.
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans."
      Smeekens S.P., Avruch A.S., Lamendola J., Chan S.J., Steiner D.F.
      Proc. Natl. Acad. Sci. U.S.A. 88:340-344(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiNEC1_MUSCO
    AccessioniPrimary (citable) accession number: P63240
    Secondary accession number(s): P21662, P22546
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3