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P63239

- NEC1_MOUSE

UniProt

P63239 - NEC1_MOUSE

Protein

Neuroendocrine convertase 1

Gene

Pcsk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

    Catalytic activityi

    Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

    Cofactori

    Calcium.

    pH dependencei

    Optimum pH is 5.5-6.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei167 – 1671Charge relay systemBy similarity
    Active sitei208 – 2081Charge relay systemBy similarity
    Active sitei382 – 3821Charge relay systemBy similarity

    GO - Molecular functioni

    1. endopeptidase activity Source: BHF-UCL
    2. serine-type endopeptidase activity Source: BHF-UCL

    GO - Biological processi

    1. peptide biosynthetic process Source: BHF-UCL
    2. peptide hormone processing Source: BHF-UCL
    3. protein processing Source: BHF-UCL

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_206713. NGF processing.
    REACT_207837. Insulin processing.

    Protein family/group databases

    MEROPSiS08.072.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuroendocrine convertase 1 (EC:3.4.21.93)
    Short name:
    NEC 1
    Alternative name(s):
    Furin homolog
    PC3
    Prohormone convertase 1
    Propeptide-processing protease
    Proprotein convertase 1
    Short name:
    PC1
    Gene namesi
    Name:Pcsk1
    Synonyms:Att-1, Nec-1, Nec1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:97511. Pcsk1.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesicle
    Note: Localized in the secretion granules.

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: Reactome
    2. extracellular space Source: BHF-UCL
    3. Golgi apparatus Source: RefGenome
    4. secretory granule lumen Source: Reactome
    5. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Propeptidei28 – 11083Sequence AnalysisPRO_0000027059Add
    BLAST
    Chaini111 – 753643Neuroendocrine convertase 1PRO_0000027060Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi225 ↔ 374By similarity
    Disulfide bondi317 ↔ 347By similarity
    Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi467 ↔ 494By similarity
    Glycosylationi645 – 6451N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP63239.
    PaxDbiP63239.
    PRIDEiP63239.

    PTM databases

    PhosphoSiteiP63239.

    Miscellaneous databases

    PMAP-CutDBP63239.

    Expressioni

    Gene expression databases

    BgeeiP63239.
    CleanExiMM_PCSK1.
    GenevestigatoriP63239.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-48841N.

    Structurei

    Secondary structure

    1
    753
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi35 – 384
    Helixi43 – 5311
    Beta strandi62 – 654
    Beta strandi67 – 715
    Beta strandi77 – 793
    Helixi89 – 946
    Beta strandi97 – 1004
    Beta strandi713 – 7164
    Beta strandi718 – 7214
    Helixi722 – 7265
    Turni727 – 7304
    Helixi741 – 7488
    Turni749 – 7513

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1KN6NMR-A28-110[»]
    2KDTNMR-A711-753[»]
    2KE3NMR-A711-753[»]
    ProteinModelPortaliP63239.
    SMRiP63239. Positions 31-103, 123-597, 711-753.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP63239.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini162 – 451290Peptidase S8Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S8 family. Furin subfamily.Curated
    Contains 1 peptidase S8 domain.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4935.
    HOGENOMiHOG000192536.
    HOVERGENiHBG008705.
    InParanoidiP63239.
    KOiK01359.
    OMAiNNPGWKK.
    OrthoDBiEOG7BW0JD.
    PhylomeDBiP63239.
    TreeFamiTF314277.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR022005. Proho_convert.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view]
    PANTHERiPTHR10795. PTHR10795. 1 hit.
    PfamiPF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    PF12177. Proho_convert. 1 hit.
    [Graphical view]
    PRINTSiPR00723. SUBTILISIN.
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P63239-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQRGWTLQC TAFAFFCVWC ALNSVKAKRQ FVNEWAAEIP GGQEAASAIA    50
    EELGYDLLGQ IGSLENHYLF KHKSHPRRSR RSALHITKRL SDDDRVTWAE 100
    QQYEKERSKR SVQKDSALDL FNDPMWNQQW YLQDTRMTAA LPKLDLHVIP 150
    VWEKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY 200
    DLTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE 250
    ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG 300
    SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST 350
    LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP 400
    NLTWRDMQHL VVWTSEYDPL ASNPGWKKNG AGLMVNSRFG FGLLNAKALV 450
    DLADPRTWRN VPEKKECVVK DNNFEPRALK ANGEVIVEIP TRACEGQENA 500
    IKSLEHVQFE ATIEYSRRGD LHVTLTSAVG TSTVLLAERE RDTSPNGFKN 550
    WDFMSVHTWG ENPVGTWTLK ITDMSGRMQN EGRIVNWKLI LHGTSSQPEH 600
    MKQPRVYTSY NTVQNDRRGV EKMVNVVEKR PTQKSLNGNL LVPKNSSSSN 650
    VEGRRDEQVQ GTPSKAMLRL LQSAFSKNAL SKQSPKKSPS AKLSIPYESF 700
    YEALEKLNKP SKLEGSEDSL YSDYVDVFYN TKPYKHRDDR LLQALMDILN 750
    EEN 753
    Length:753
    Mass (Da):84,174
    Last modified:October 11, 2004 - v1
    Checksum:i04239C7B6385382E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti112 – 1121V → F in CAA40368. (PubMed:1657897)Curated
    Sequence conflicti117 – 1171A → P in CAA40368. (PubMed:1657897)Curated
    Sequence conflicti122 – 1221N → T in CAA40368. (PubMed:1657897)Curated
    Sequence conflicti128 – 1281Q → H in CAA40368. (PubMed:1657897)Curated
    Sequence conflicti282 – 2821R → K in CAA40368. (PubMed:1657897)Curated
    Sequence conflicti330 – 3301S → L in AAA39375. (PubMed:2017186)Curated
    Sequence conflicti732 – 7321K → E in CAA40368. (PubMed:1657897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M69196 mRNA. Translation: AAA39732.1.
    X57088 mRNA. Translation: CAA40368.1.
    M58589 mRNA. Translation: AAA39894.1.
    M55668 mRNA. Translation: AAA39375.1. Sequence problems.
    CCDSiCCDS26649.1.
    PIRiJX0171. KXMSC1.
    RefSeqiNP_038656.1. NM_013628.2.
    XP_006517216.1. XM_006517153.1.
    XP_006517217.1. XM_006517154.1.
    XP_006517218.1. XM_006517155.1.
    UniGeneiMm.1333.
    Mm.394672.

    Genome annotation databases

    EnsembliENSMUST00000022075; ENSMUSP00000022075; ENSMUSG00000021587.
    GeneIDi18548.
    KEGGimmu:18548.
    UCSCiuc007rfs.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M69196 mRNA. Translation: AAA39732.1 .
    X57088 mRNA. Translation: CAA40368.1 .
    M58589 mRNA. Translation: AAA39894.1 .
    M55668 mRNA. Translation: AAA39375.1 . Sequence problems.
    CCDSi CCDS26649.1.
    PIRi JX0171. KXMSC1.
    RefSeqi NP_038656.1. NM_013628.2.
    XP_006517216.1. XM_006517153.1.
    XP_006517217.1. XM_006517154.1.
    XP_006517218.1. XM_006517155.1.
    UniGenei Mm.1333.
    Mm.394672.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1KN6 NMR - A 28-110 [» ]
    2KDT NMR - A 711-753 [» ]
    2KE3 NMR - A 711-753 [» ]
    ProteinModelPortali P63239.
    SMRi P63239. Positions 31-103, 123-597, 711-753.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48841N.

    Protein family/group databases

    MEROPSi S08.072.

    PTM databases

    PhosphoSitei P63239.

    Proteomic databases

    MaxQBi P63239.
    PaxDbi P63239.
    PRIDEi P63239.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022075 ; ENSMUSP00000022075 ; ENSMUSG00000021587 .
    GeneIDi 18548.
    KEGGi mmu:18548.
    UCSCi uc007rfs.1. mouse.

    Organism-specific databases

    CTDi 5122.
    MGIi MGI:97511. Pcsk1.

    Phylogenomic databases

    eggNOGi COG4935.
    HOGENOMi HOG000192536.
    HOVERGENi HBG008705.
    InParanoidi P63239.
    KOi K01359.
    OMAi NNPGWKK.
    OrthoDBi EOG7BW0JD.
    PhylomeDBi P63239.
    TreeFami TF314277.

    Enzyme and pathway databases

    Reactomei REACT_206713. NGF processing.
    REACT_207837. Insulin processing.

    Miscellaneous databases

    EvolutionaryTracei P63239.
    NextBioi 294338.
    PMAP-CutDB P63239.
    PROi P63239.
    SOURCEi Search...

    Gene expression databases

    Bgeei P63239.
    CleanExi MM_PCSK1.
    Genevestigatori P63239.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR022005. Proho_convert.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view ]
    PANTHERi PTHR10795. PTHR10795. 1 hit.
    Pfami PF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    PF12177. Proho_convert. 1 hit.
    [Graphical view ]
    PRINTSi PR00723. SUBTILISIN.
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and functional expression of a mammalian prohormone processing enzyme, murine prohormone convertase 1."
      Korner J., Chun J., Harter D., Axel R.
      Proc. Natl. Acad. Sci. U.S.A. 88:6834-6838(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pituitary.
    2. "Cloning and functional expression of a novel endoprotease involved in prohormone processing at dibasic sites."
      Nakayama K., Hosaka M., Hatsuzawa K., Murakami K.
      J. Biochem. 109:803-806(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: LAF1.
    3. "Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, Furin, and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2."
      Seidah N.G., Marcinkiewicz M., Benjannet S., Gaspar L., Beaubien G., Mattei M.-G., Lazure C., Mbikay M., Chretien M.
      Mol. Endocrinol. 5:111-122(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Pituitary.
    4. "Purification and characterization of the prohormone convertase PC1(PC3)."
      Zhou Y., Lindberg I.
      J. Biol. Chem. 268:5615-5623(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 111-120.
      Tissue: Ovary.
    5. "cDNA sequence of two distinct pituitary proteins homologous to Kex2 and furin gene products: tissue-specific mRNAs encoding candidates for pro-hormone processing proteinases."
      Seidah N.G., Gaspar L., Mion P., Marcinkiewicz M., Mbikay M., Chretien M.
      DNA Cell Biol. 9:415-424(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 214-478.
      Tissue: Pituitary.
    6. "Solution structure of the pro-hormone convertase 1 pro-domain from Mus musculus."
      Tangrea M.A., Bryan P.N., Sari N., Orban J.
      J. Mol. Biol. 320:801-812(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 28-110.

    Entry informationi

    Entry nameiNEC1_MOUSE
    AccessioniPrimary (citable) accession number: P63239
    Secondary accession number(s): P21662, P22546
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3