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Protein

Neuroendocrine convertase 1

Gene

Pcsk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.1 Publication

Catalytic activityi

Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Cofactori

pH dependencei

Optimum pH is 5.5-6.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei167Charge relay systemBy similarity1
Active sitei208Charge relay systemBy similarity1
Active sitei382Charge relay systemBy similarity1

GO - Molecular functioni

  • endopeptidase activity Source: BHF-UCL
  • identical protein binding Source: IntAct
  • serine-type endopeptidase activity Source: BHF-UCL

GO - Biological processi

  • peptide biosynthetic process Source: BHF-UCL
  • peptide hormone processing Source: BHF-UCL
  • protein processing Source: BHF-UCL

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium

Enzyme and pathway databases

BRENDAi3.4.21.93 3474
ReactomeiR-MMU-209952 Peptide hormone biosynthesis
R-MMU-422085 Synthesis, secretion, and deacylation of Ghrelin

Protein family/group databases

MEROPSiS08.072

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 1 (EC:3.4.21.93)
Short name:
NEC 1
Alternative name(s):
Furin homolog
PC3
Prohormone convertase 1
Propeptide-processing protease
Proprotein convertase 1
Short name:
PC1
Gene namesi
Name:Pcsk1
Synonyms:Att-1, Nec-1, Nec1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:97511 Pcsk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle

Pathology & Biotechi

Disruption phenotypei

Increase in unprocessed AGRP.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000002705928 – 110Sequence analysisAdd BLAST83
ChainiPRO_0000027060111 – 753Neuroendocrine convertase 1Add BLAST643

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi225 ↔ 374By similarity
Disulfide bondi317 ↔ 347By similarity
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi467 ↔ 494By similarity
Glycosylationi645N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP63239
PeptideAtlasiP63239
PRIDEiP63239

PTM databases

iPTMnetiP63239
PhosphoSitePlusiP63239

Miscellaneous databases

PMAP-CutDBiP63239

Expressioni

Gene expression databases

BgeeiENSMUSG00000021587
CleanExiMM_PCSK1
ExpressionAtlasiP63239 baseline and differential
GenevisibleiP63239 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15770815,EBI-15770815

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-48841N
STRINGi10090.ENSMUSP00000022075

Structurei

Secondary structure

1753
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 38Combined sources4
Helixi43 – 53Combined sources11
Beta strandi62 – 65Combined sources4
Beta strandi67 – 71Combined sources5
Beta strandi77 – 79Combined sources3
Helixi89 – 94Combined sources6
Beta strandi97 – 100Combined sources4
Beta strandi713 – 716Combined sources4
Beta strandi718 – 721Combined sources4
Helixi722 – 726Combined sources5
Turni727 – 730Combined sources4
Helixi741 – 748Combined sources8
Turni749 – 751Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KN6NMR-A28-110[»]
2KDTNMR-A711-753[»]
2KE3NMR-A711-753[»]
ProteinModelPortaliP63239
SMRiP63239
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP63239

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 451Peptidase S8Add BLAST290
Domaini460 – 597P/Homo BPROSITE-ProRule annotationAdd BLAST138

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3525 Eukaryota
COG1404 LUCA
COG4935 LUCA
GeneTreeiENSGT00750000117358
HOGENOMiHOG000192536
HOVERGENiHBG008705
InParanoidiP63239
KOiK01359
OMAiFEPRALK
OrthoDBiEOG091G05HI
PhylomeDBiP63239
TreeFamiTF314277

Family and domain databases

CDDicd04059 Peptidases_S8_Protein_converta, 1 hit
Gene3Di2.60.120.260, 1 hit
3.30.70.850, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR034182 Kexin/furin
IPR002884 P_dom
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR022005 Proho_convert
IPR032815 S8_pro-domain
IPR038466 S8_pro-domain_sf
PfamiView protein in Pfam
PF01483 P_proprotein, 1 hit
PF00082 Peptidase_S8, 1 hit
PF12177 Proho_convert, 1 hit
PF16470 S8_pro-domain, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF49785 SSF49785, 1 hit
SSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51829 P_HOMO_B, 1 hit
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P63239-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQRGWTLQC TAFAFFCVWC ALNSVKAKRQ FVNEWAAEIP GGQEAASAIA
60 70 80 90 100
EELGYDLLGQ IGSLENHYLF KHKSHPRRSR RSALHITKRL SDDDRVTWAE
110 120 130 140 150
QQYEKERSKR SVQKDSALDL FNDPMWNQQW YLQDTRMTAA LPKLDLHVIP
160 170 180 190 200
VWEKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY
210 220 230 240 250
DLTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE
260 270 280 290 300
ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG
310 320 330 340 350
SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST
360 370 380 390 400
LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP
410 420 430 440 450
NLTWRDMQHL VVWTSEYDPL ASNPGWKKNG AGLMVNSRFG FGLLNAKALV
460 470 480 490 500
DLADPRTWRN VPEKKECVVK DNNFEPRALK ANGEVIVEIP TRACEGQENA
510 520 530 540 550
IKSLEHVQFE ATIEYSRRGD LHVTLTSAVG TSTVLLAERE RDTSPNGFKN
560 570 580 590 600
WDFMSVHTWG ENPVGTWTLK ITDMSGRMQN EGRIVNWKLI LHGTSSQPEH
610 620 630 640 650
MKQPRVYTSY NTVQNDRRGV EKMVNVVEKR PTQKSLNGNL LVPKNSSSSN
660 670 680 690 700
VEGRRDEQVQ GTPSKAMLRL LQSAFSKNAL SKQSPKKSPS AKLSIPYESF
710 720 730 740 750
YEALEKLNKP SKLEGSEDSL YSDYVDVFYN TKPYKHRDDR LLQALMDILN

EEN
Length:753
Mass (Da):84,174
Last modified:October 11, 2004 - v1
Checksum:i04239C7B6385382E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112V → F in CAA40368 (PubMed:1657897).Curated1
Sequence conflicti117A → P in CAA40368 (PubMed:1657897).Curated1
Sequence conflicti122N → T in CAA40368 (PubMed:1657897).Curated1
Sequence conflicti128Q → H in CAA40368 (PubMed:1657897).Curated1
Sequence conflicti282R → K in CAA40368 (PubMed:1657897).Curated1
Sequence conflicti330S → L in AAA39375 (PubMed:2017186).Curated1
Sequence conflicti732K → E in CAA40368 (PubMed:1657897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69196 mRNA Translation: AAA39732.1
X57088 mRNA Translation: CAA40368.1
M58589 mRNA Translation: AAA39894.1
M55668 mRNA Translation: AAA39375.1 Sequence problems.
CCDSiCCDS26649.1
PIRiJX0171 KXMSC1
RefSeqiNP_038656.1, NM_013628.2
XP_006517216.1, XM_006517153.2
XP_006517217.1, XM_006517154.3
XP_006517218.1, XM_006517155.2
UniGeneiMm.1333
Mm.394672

Genome annotation databases

EnsembliENSMUST00000022075; ENSMUSP00000022075; ENSMUSG00000021587
GeneIDi18548
KEGGimmu:18548
UCSCiuc007rfs.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNEC1_MOUSE
AccessioniPrimary (citable) accession number: P63239
Secondary accession number(s): P21662, P22546
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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