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Protein

Probable glutamate/gamma-aminobutyrate antiporter

Gene

gadC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in glutamate-dependent acid resistance. Imports glutamate inside the cell while simultaneously exporting to the periplasm the GABA produced by GadA and GadB. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria.

GO - Molecular functioni

  • antiporter activity Source: EcoCyc
  • L-amino acid transmembrane transporter activity Source: GO_Central

GO - Biological processi

  • intracellular pH elevation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Antiport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:XASA-MONOMER.
ECOL316407:JW1487-MONOMER.
MetaCyc:XASA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate/gamma-aminobutyrate antiporter
Alternative name(s):
Extreme acid sensitivity protein
Gene namesi
Name:gadC
Synonyms:acsA, xasA
Ordered Locus Names:b1492, JW1487
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12456. gadC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicSequence analysisAdd BLAST14
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Topological domaini36 – 41PeriplasmicSequence analysis6
Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
Topological domaini63 – 93CytoplasmicSequence analysisAdd BLAST31
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Topological domaini115 – 127PeriplasmicSequence analysisAdd BLAST13
Transmembranei128 – 148HelicalSequence analysisAdd BLAST21
Topological domaini149 – 157CytoplasmicSequence analysis9
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 200PeriplasmicSequence analysisAdd BLAST22
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
Topological domaini222 – 239CytoplasmicSequence analysisAdd BLAST18
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Topological domaini261 – 291PeriplasmicSequence analysisAdd BLAST31
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Topological domaini313 – 335CytoplasmicSequence analysisAdd BLAST23
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21
Topological domaini357 – 366PeriplasmicSequence analysis10
Transmembranei367 – 387HelicalSequence analysisAdd BLAST21
Topological domaini388 – 412CytoplasmicSequence analysisAdd BLAST25
Transmembranei413 – 433HelicalSequence analysisAdd BLAST21
Topological domaini434 – 445PeriplasmicSequence analysisAdd BLAST12
Transmembranei446 – 466HelicalSequence analysisAdd BLAST21
Topological domaini467 – 511CytoplasmicSequence analysisAdd BLAST45

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002130401 – 511Probable glutamate/gamma-aminobutyrate antiporterAdd BLAST511

Proteomic databases

PaxDbiP63235.
PRIDEiP63235.

Expressioni

Inductioni

By acidic conditions. Expression is regulated by a complex system involving RpoS, cAMP, CRP, EvgAS, H-NS, GadE, GadW and GadX. The level of involvement for each regulator varies depending upon the growth phase and the medium.6 Publications

Interactioni

Protein-protein interaction databases

BioGridi4260789. 7 interactors.
DIPiDIP-48121N.
STRINGi511145.b1492.

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 21Combined sources7
Helixi23 – 26Combined sources4
Helixi29 – 31Combined sources3
Helixi32 – 36Combined sources5
Helixi37 – 40Combined sources4
Helixi41 – 52Combined sources12
Helixi54 – 65Combined sources12
Turni68 – 70Combined sources3
Helixi75 – 83Combined sources9
Helixi85 – 102Combined sources18
Helixi104 – 115Combined sources12
Turni116 – 119Combined sources4
Helixi121 – 123Combined sources3
Helixi129 – 146Combined sources18
Beta strandi149 – 151Combined sources3
Helixi152 – 155Combined sources4
Helixi157 – 162Combined sources6
Helixi165 – 178Combined sources14
Turni201 – 206Combined sources6
Helixi207 – 214Combined sources8
Helixi217 – 220Combined sources4
Helixi222 – 224Combined sources3
Beta strandi225 – 228Combined sources4
Turni231 – 233Combined sources3
Helixi234 – 257Combined sources24
Helixi262 – 264Combined sources3
Beta strandi267 – 269Combined sources3
Helixi271 – 279Combined sources9
Beta strandi280 – 282Combined sources3
Helixi284 – 288Combined sources5
Helixi289 – 308Combined sources20
Helixi311 – 315Combined sources5
Helixi318 – 321Combined sources4
Helixi322 – 324Combined sources3
Beta strandi326 – 328Combined sources3
Beta strandi334 – 336Combined sources3
Helixi340 – 357Combined sources18
Beta strandi358 – 361Combined sources4
Helixi362 – 395Combined sources34
Beta strandi401 – 403Combined sources3
Helixi410 – 429Combined sources20
Beta strandi437 – 440Combined sources4
Helixi445 – 465Combined sources21
Beta strandi478 – 480Combined sources3
Beta strandi484 – 486Combined sources3
Turni496 – 498Combined sources3
Beta strandi501 – 507Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DJIX-ray3.19A/B1-511[»]
4DJKX-ray3.10A/B1-511[»]
ProteinModelPortaliP63235.
SMRiP63235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105E5D. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000058004.
InParanoidiP63235.
KOiK20265.
OMAiVYKINPE.
PhylomeDBiP63235.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004759. Glu_antiport.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00910. 2A0307_GadC. 1 hit.

Sequencei

Sequence statusi: Complete.

P63235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSVQTGKA KQLTLLGFFA ITASMVMAVY EYPTFATSGF SLVFFLLLGG
60 70 80 90 100
ILWFIPVGLC AAEMATVDGW EEGGVFAWVS NTLGPRWGFA AISFGYLQIA
110 120 130 140 150
IGFIPMLYFV LGALSYILKW PALNEDPITK TIAALIILWA LALTQFGGTK
160 170 180 190 200
YTARIAKVGF FAGILLPAFI LIALAAIYLH SGAPVAIEMD SKTFFPDFSK
210 220 230 240 250
VGTLVVFVAF ILSYMGVEAS ATHVNEMSNP GRDYPLAMLL LMVAAICLSS
260 270 280 290 300
VGGLSIAMVI PGNEINLSAG VMQTFTVLMS HVAPEIEWTV RVISALLLLG
310 320 330 340 350
VLAEIASWIV GPSRGMYVTA QKNLLPAAFA KMNKNGVPVT LVISQLVITS
360 370 380 390 400
IALIILTNTG GGNNMSFLIA LALTVVIYLC AYFMLFIGYI VLVLKHPDLK
410 420 430 440 450
RTFNIPGGKG VKLVVAIVGL LTSIMAFIVS FLPPDNIQGD STDMYVELLV
460 470 480 490 500
VSFLVVLALP FILYAVHDRK GKANTGVTLE PINSQNAPKG HFFLHPRARS
510
PHYIVMNDKK H
Length:511
Mass (Da):55,077
Last modified:October 11, 2004 - v1
Checksum:i85DF72B82816CE33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74565.1.
AP009048 Genomic DNA. Translation: BAA15156.2.
U13204 Genomic DNA. Translation: AAB17694.1.
PIRiG64902.
RefSeqiNP_416009.1. NC_000913.3.
WP_000246019.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74565; AAC74565; b1492.
BAA15156; BAA15156; BAA15156.
GeneIDi946057.
KEGGiecj:JW1487.
eco:b1492.
PATRICi32118278. VBIEscCol129921_1559.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74565.1.
AP009048 Genomic DNA. Translation: BAA15156.2.
U13204 Genomic DNA. Translation: AAB17694.1.
PIRiG64902.
RefSeqiNP_416009.1. NC_000913.3.
WP_000246019.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DJIX-ray3.19A/B1-511[»]
4DJKX-ray3.10A/B1-511[»]
ProteinModelPortaliP63235.
SMRiP63235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260789. 7 interactors.
DIPiDIP-48121N.
STRINGi511145.b1492.

Proteomic databases

PaxDbiP63235.
PRIDEiP63235.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74565; AAC74565; b1492.
BAA15156; BAA15156; BAA15156.
GeneIDi946057.
KEGGiecj:JW1487.
eco:b1492.
PATRICi32118278. VBIEscCol129921_1559.

Organism-specific databases

EchoBASEiEB2350.
EcoGeneiEG12456. gadC.

Phylogenomic databases

eggNOGiENOG4105E5D. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000058004.
InParanoidiP63235.
KOiK20265.
OMAiVYKINPE.
PhylomeDBiP63235.

Enzyme and pathway databases

BioCyciEcoCyc:XASA-MONOMER.
ECOL316407:JW1487-MONOMER.
MetaCyc:XASA-MONOMER.

Miscellaneous databases

PROiP63235.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004759. Glu_antiport.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00910. 2A0307_GadC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGADC_ECOLI
AccessioniPrimary (citable) accession number: P63235
Secondary accession number(s): P39183
, P76131, P77384, Q54152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The glutamate analog L-trans-pyrrolidine-2,4-dicarboxylic acid (L-PDC) blocks the uptake of glutamate by selective inhibition.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.