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Protein

D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase

Gene

gmhB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Converts the D-glycero-beta-D-manno-heptose 1,7-bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1-phosphate by removing the phosphate group at the C-7 position.By similarity

Catalytic activityi

D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H2O = D-glycero-beta-D-manno-heptose 1-phosphate + phosphate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE)
  2. D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (gmhB)
  3. Bifunctional protein HldE (hldE)
  4. ADP-L-glycero-D-manno-heptose-6-epimerase (hldD)
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11NucleophileBy similarity1
Metal bindingi11MagnesiumBy similarity1
Active sitei13Proton donorBy similarity1
Metal bindingi13Magnesium; via carbonyl oxygenBy similarity1
Sitei53Stabilizes the phosphoryl groupBy similarity1
Metal bindingi92ZincBy similarity1
Metal bindingi94Zinc; via pros nitrogenBy similarity1
Metal bindingi107ZincBy similarity1
Metal bindingi109ZincBy similarity1
Sitei110Contributes to substrate recognitionBy similarity1
Sitei111Stabilizes the phosphoryl groupBy similarity1
Metal bindingi136MagnesiumBy similarity1
Metal bindingi137MagnesiumBy similarity1
Binding sitei137SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCarbohydrate metabolism, Lipopolysaccharide biosynthesis
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00356; UER00438.
UPA00958.

Names & Taxonomyi

Protein namesi
Recommended name:
D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase (EC:3.1.3.82)
Alternative name(s):
D,D-heptose 1,7-bisphosphate phosphatase
Short name:
HBP phosphatase
Gene namesi
Name:gmhB
Ordered Locus Names:Z0212, ECs0202
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002093901 – 191D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphataseAdd BLAST191

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi155864.Z0212.

Structurei

3D structure databases

ProteinModelPortaliP63229.
SMRiP63229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 13Substrate bindingBy similarity3
Regioni19 – 22Substrate bindingBy similarity4
Regioni53 – 56Substrate bindingBy similarity4
Regioni110 – 111Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the GmhB family.Curated

Phylogenomic databases

eggNOGiENOG4108ZI0. Bacteria.
COG0241. LUCA.
HOGENOMiHOG000016501.
KOiK03273.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiView protein in InterPro
IPR023214. HAD-like_dom.
IPR006549. HAD-SF_hydro_IIIA.
IPR004446. Heptose_bisP_phosphatase.
IPR006543. Histidinol-phos.
PIRSFiPIRSF004682. GmhB. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR00213. GmhB_yaeD. 1 hit.
TIGR01662. HAD-SF-IIIA. 1 hit.
TIGR01656. Histidinol-ppas. 1 hit.

Sequencei

Sequence statusi: Complete.

P63229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSVPAIFL DRDGTINVDH GYVHEIDNFE FIDGVIDAMR ELKKMGFALV
60 70 80 90 100
VVTNQSGIAR GKFTEAQFET LTEWMDWSLA DRDVDLDGIY YCPHHPQGSV
110 120 130 140 150
EEFRQVCDCR KPHPGMLLSA RDYLHIDMAA SYMVGDKLED MQAAVAANVG
160 170 180 190
TKVLVRTGKP ITPEAENAAD WVLNSLADLP QAIKKQQKPA Q
Length:191
Mass (Da):21,294
Last modified:September 27, 2004 - v1
Checksum:iE7814B34A23128FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035926 Genomic DNA. Translation: BAA93568.1.
AE005174 Genomic DNA. Translation: AAG54502.1.
BA000007 Genomic DNA. Translation: BAB33625.1.
PIRiB85505.
B90654.
RefSeqiNP_308229.1. NC_002695.1.
WP_001140187.1. NZ_MWVM01000012.1.

Genome annotation databases

EnsemblBacteriaiAAG54502; AAG54502; Z0212.
BAB33625; BAB33625; BAB33625.
GeneIDi913974.
KEGGiece:Z0212.
ecs:ECs0202.
PATRICifig|386585.9.peg.306.

Similar proteinsi

Entry informationi

Entry nameiGMHBB_ECO57
AccessioniPrimary (citable) accession number: P63229
Secondary accession number(s): P31546
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: August 30, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families