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P63226 (GMHA_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoheptose isomerase

EC=5.3.1.28
Alternative name(s):
Sedoheptulose 7-phosphate isomerase
Gene names
Name:gmhA
Ordered Locus Names:c0372
OrganismEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]
Taxonomic identifier199310 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length192 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate By similarity. HAMAP-Rule MF_00067

Catalytic activity

D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate. HAMAP-Rule MF_00067

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00067

Pathway

Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1. HAMAP-Rule MF_00067

Bacterial outer membrane biogenesis; LPS core biosynthesis. HAMAP-Rule MF_00067

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00067

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00067.

Miscellaneous

The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate By similarity.

Sequence similarities

Belongs to the SIS family. GmhA subfamily.

Contains 1 SIS domain.

Sequence caution

The sequence AAN78853.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 192192Phosphoheptose isomerase HAMAP-Rule MF_00067
PRO_0000136528

Regions

Domain37 – 192156SIS
Region52 – 543Substrate binding By similarity
Region93 – 942Substrate binding By similarity
Region119 – 1213Substrate binding By similarity

Sites

Metal binding611Zinc By similarity
Metal binding651Zinc By similarity
Metal binding1721Zinc By similarity
Metal binding1801Zinc By similarity
Binding site651Substrate By similarity
Binding site1241Substrate By similarity
Binding site1721Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P63226 [UniParc].

Last modified September 27, 2004. Version 1.
Checksum: 7A2C05E1079108B4

FASTA19220,815
        10         20         30         40         50         60 
MYQDLIRNEL NEAAETLANF LKDDANIHAI QRAAVLLADS FKAGGKVLSC GNGGSHCDAM 

        70         80         90        100        110        120 
HFAEELTGRY RENRPGYPAI AISDVSHISC VGNDFGFNDI FSRYVEAVGR EGDVLLGIST 

       130        140        150        160        170        180 
SGNSANVIKA IAAAREKGMK VITLTGKDGG KMAGTADIEI RVPHFGYADR IQEIHIKVIH 

       190 
ILIQLIEKEM VK 

« Hide

References

[1]"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014075 Genomic DNA. Translation: AAN78853.1. Different initiation.
RefSeqNP_752309.2. NC_004431.1.

3D structure databases

ProteinModelPortalP63226.
SMRP63226. Positions 1-192.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING199310.c0372.

Proteomic databases

PRIDEP63226.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN78853; AAN78853; c0372.
GeneID1036332.
KEGGecc:c0372.
PATRIC18278796. VBIEscCol75197_0345.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000237571.
KOK03271.
OMAFLAHKEA.
OrthoDBEOG6384PC.
ProtClustDBPRK00414.

Enzyme and pathway databases

UniPathwayUPA00041; UER00436.
UPA00958.

Family and domain databases

HAMAPMF_00067. GmhA.
InterProIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsTIGR00441. gmhA. 1 hit.
PROSITEPS51464. SIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGMHA_ECOL6
AccessionPrimary (citable) accession number: P63226
Secondary accession number(s): P51001
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways